The
TRK-fused gene (
TFG in human,
Tfg in rat) was originally identified in human papillary thyroid cancer as a chimeric form of the
NTRK1 gene. It has been reported that the gene product (TFG) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. However, no information regarding the localization of
Tfg in rat tissues is available. In this study, we investigated the expression of
Tfg mRNA in normal rat tissues using reverse transcription-polymerase chain reaction (RT-PCR). We also produced an antibody against
Tfg gene products and examined the localization of TFG in the rat brain and retina. The RT-PCR experiments demonstrated that two types of
Tfg mRNA were expressed in rat tissues: the conventional form of
Tfg (
cTfg) and a novel variant form, retinal
Tfg (
rTfg). RT-PCR analyses demonstrated that
cTfg was ubiquitously expressed in rat tissues, while
rTfg was predominantly expressed in the brain and retina. Western blot analysis demonstrated two bands with molecular weights of about 30 kDa and 50 kDa in the rat brain. Immunohistochemistry indicated that TFG proteins were predominantly expressed by neurons in the brain. In the rat retina, intense TFG-immunoreactivity was detected in the layer of rods and cones and the outer plexiform layer.
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