巻号一覧
5 巻 (1972)
- 4 号 p. 195-
- 3 号 p. 117-
- 2 号 p. 57-
- 1 号 p. 1-
5 巻, 2 号
選択された号の論文の6件中1～6を表示しています
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TERUO IWAMASA, TADAO TAKEUCHI1972 年 5 巻 2 号 p. 57-70
発行日: 1972年
公開日: 2009/10/28
ジャーナル フリーThe influences of primer glycogen on the iodine staining procedure for histochemical demonstration of phosphorylase were studied by biochemical, physico-chemical and electrophoretical techniques. The low concentration of primer present in the in vitro reaction gave the blue color with iodine staining for polysaccharide synthesized by phosphorylase and the high concentration of primer glycogen exhibited the reddish color. The muscle type glycogen had the stronger affinity to the iodine solution than the liver type. Polygucose which was synthesized by the simultaneous action of phosphorylase and glycosyl transferase was stained reddish with iodine. Each polyglucose which was synthesized by the activity of isoenzymes of the liver, muscle and hepatoma AH13 stained similarly with iodine following electrophoresis, in spite of a different coloration in the histochemical procedure. In the latter, it is likely that polyglucose synthesized in the intracellular conditions of the incubated tissue might be influenced by the activity of enzymes as well as the concentration of primer glycogen and it's physico-chemical structure抄録全体を表示PDF形式でダウンロード (6254K) -
IKUKO NAGATSU, YUMIKO SOTOKAWA, MASAO SANO1972 年 5 巻 2 号 p. 71-79
発行日: 1972年
公開日: 2009/10/28
ジャーナル フリーOudenone (a tyrosine hydroxylase inhibitor) and fusaric acid (a dopamine-β-hydroxylase inhibitor) had some effects on fine structures of the adrenal medulla. The Golgi apparatus was not seen to be well developed, and the formation of epinephrine and norepinephrine granules appeared to be decreased. The limiting membrane of norepinephrine granules showed a tendency of fusing each other, and the density of epinephrine granules was seen to be decreased. Both epinephrine and norepinephrine granules showed a reduction in size of their dense core.
It is concluded that oudenone and fusaric acid may have some inhibjtory effects on epinephrine and norepinephrine biosynthesis in the mouse adrenal medulla. These results agree well with the biochemical data presented previously.抄録全体を表示PDF形式でダウンロード (12599K) -
SHUNTA HIROSE, MASAYUKI YASUTOMI, NOBUHIRO MURAI, ZENJI IWASA, MASAAKI ...1972 年 5 巻 2 号 p. 80-89
発行日: 1972年
公開日: 2009/10/28
ジャーナル フリーVarious methods have been introduced for chemical detection of sialic acid. Among those methods we applied resorcinol reaction, orcinol reaction (Bial) and thiobarbituric acid reaction to histochemical study as they appeared to be useful. As the result, it was found that only orcinol reaction (Bial) shows reaction on specimen. When orcinol reaction was conducted by using pure reagents, fructose, sorbose, tryptophan and sialic acid (N-acetylneuraminic acid) indicated positive reaction. Fructose and sorbose were discriminated from sialic acid because of their color and its quick fading. Discrimination of tryptophan from sialic acid was possible by tryptophan staining by Serra's method and tetrazonium method as its reaction part is different. It was also possible to discriminate these substances by spectrophotomter and thin-layer chromatography. It was therefore concluded that the substance which develops color in Bial's reaction on specimen is most probably sialic acid.抄録全体を表示PDF形式でダウンロード (2819K) -
SHUNTA HIROSE, MASAYUKI YASUTOMI, NOBUHIRO MURAI, ZENJI IWASA, MASAAKI ...1972 年 5 巻 2 号 p. 90-96
発行日: 1972年
公開日: 2009/10/28
ジャーナル フリーIt has been regarded almost impossible to do counter staining of tissue sections in combination with Bial's reaction, which is a direct method of demonstration of sialic acid. The reasons are; 1 reaction product which is developed in Bial's reaction is highly soluble in water and (2) since concentrated hydrochloric acid is used in Bial's reaction, the basic dyes currently used do not fit for the nuclear staining. Diezel or Ravetto, who introduced Bial's reaction into histochemistry, did not try any counter staining. In this paper, we have introduced a new method of nuclear staining which bases on the absorption of ferric ion to nuclei and the visualization of it by the formation of Prussian blue. This counter staining may be applied in combination with other histochemical procedures which should be done under a strongly acidic condition.抄録全体を表示PDF形式でダウンロード (4499K) -
YASUHIKO IBATA, YOSHIAKI NOJYO, TADAO MATSUURA, JUNZO OCHI1972 年 5 巻 2 号 p. 97-105
発行日: 1972年
公開日: 2009/10/28
ジャーナル フリーThe synaptic terminals of the ventral horn of rat spinal cords were demonstrated by electron microscopy using a modification of the zinc iodideosmium tetroxide staining technique in which zinc iodide was substituted for a mixture of zinc powder and iodine sublimate.
The best staining reaction consistent with tissue preservation was obtained by incubating specimens for 16 hours at 4°C in solutions containing 1% or 2% zinc iodide. Synaptic vesicles staining black were observed in both F and S types of synaptic terminals. With the exception of some parts of the myelin sheath no other neuronal or glial elements gave a positive reaction.
The modified technique was compared with that of other investigators and the possible nature of the reaction between the stain and the synaptic vesicles was briefly discussed.抄録全体を表示PDF形式でダウンロード (13235K) -
TERUO IWAMASA1972 年 5 巻 2 号 p. 106-114
発行日: 1972年
公開日: 2009/10/28
ジャーナル フリーAs reported previously (6, 7, 8), the glycogen particles from the rat liver and muscle appeared as the specific forms of spheroidal branching bodies. The branching structures were more clearly demonstrated in the glycogen particles from the fasted animal and these appearances were different from the aggregated structures. The chemical and physico-chemical properties of glycogens from the rat liver and muscle are demonstrated and discussed in this paper.
1. End group assay of the glycogens and β-amylase digestion degree suggested that the average chain length of the liver glycogen was longer than the muscle glycogen.
2. The viscosity of glycogen, measured by varing the temperature from 10°C to 70°C, showed the non-aggregated structure of the glycogen. particles.
3. The optical rotartory dispersion and circular dichroism spectra of the glycogen were measured using an addition of iodine solution, and only the muscle glycogen showed the Cotton effect.
4. The sedimentation coefficient of the liver glycogen showed the larger s value than the muscle one, and the relationship between s value and concentration of the glycogen was discussed.抄録全体を表示PDF形式でダウンロード (5120K)
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