All issues
Volume 9 (1976)
- Issue 4 Pages 269-
- Issue 3 Pages 197-
- Issue 2 Pages 107-
- Issue 1 Pages 1-
Volume 9, Issue 1
Displaying 1-13 of 13 articles from this issue
- |<
- <
- 1
- >
- >|
-
QUANTITATIVE EVALUATION OF HEPATIC GLYCOGEN BY MEANS OF HISTOPHOTOMETRY ON SEMITHIN SECTIONS OF EPON-EMBEDDED TISSUEJEAN-MARIE VERNIER, MARIE-FRANCE SIRE1976 Volume 9 Issue 1 Pages 1-11
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSOur aim is to make a quantitative evaluation of glycogen possible on sernithin sections of hepatic tissue, epon embedded and prepared for an ultrastructural study that is not limited to glycogen aspects only.
The work has been done on Salmo gairdneri. On each liver both a biochemical estimate and histophotometric measurements are performed. For the histophotometric study, the tissue is fixed with 4% paraformaldehyde in 0, 15 M sodium cacodylate buffer (pH 7.3) ans postfixed with osmium tetroxyde. The 1μ sections are stained with P.A.S. in well defined conditions. A counterstaining with Erlich's hematoxylin can be performed for a later morphological study. As for the ultrastructural study, thin sections are either contrasted with uranyl acetate and lead citrate or undergo a silver proteinate treatment.
For the biochemical study, KOH digestion of the liver is done and the alcohol precipitated glycogen is estimated with anthrone reagent. There is a good agreement between the results of the histophotometric method and those of the biochemical estimates.
The validity of these methods is then discussed.View full abstractDownload PDF (5272K) -
MASATOYO AKIYOSHI, SABURO YANO, HOZUMI NAKADA1976 Volume 9 Issue 1 Pages 12-29
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSIn the living hair cells of the inner ears in anesthetized guinea pigs, Nitro-BT and phosphate buffer mixture can penetrate into the hair cells and undergoes reduction in absence of the specific substrate, resulting in the production of formazan. This Nitro-BT reducibility of the hair cells is demonstrated in the inner ears taken from guinea pigs immediately after decapitation, too. In the present study, effect of freezing to temperatures from 0° to -30°C for 5 and 20 minutes followed by thawing on the Nitro-BT reducibility and SDH activity of the hair cells were investigated histochemically by perfusion with incubation medium. Freezing at -10°C and below for 20 minutes affected severely the Nitro-BT reducibility of the hair cells in the vestibular organs, resulting in conspicuous decrease in the formazan production. There was slight to moderate decrease in SDH activity of the hair cells in the vestibular organs after freezing to 0°C and below for 20 minutes. The present study suggests that the activities of the electron transport system and related dehydrogenase system in the hair cells of the vestibular organs are more sensitive to freezing injury than in the hair cells of the organ of Corti.View full abstractDownload PDF (12944K) -
PART 2; ULTRAMICROCHEMICAL DETERMINATION OF NADP-DEPENDENT ISOCITRATE AND GLUCOSE-6-PHOSPHATE DEHYDROGENASE IN THE LOBULES OF NORMAL AND INJURED HUMAN LIVERKUNIJIRO SUZUKI1976 Volume 9 Issue 1 Pages 30-36
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSQuantitative levels of glucose-6-phosphate dehydrogenase (G6PD) and isocitrate dehydrogenase (ICD) were determined in normal and injured human livers by the ultramicrochemical method of Lowry. G6PD in the normal hepatic lobule showed a central-portal gradient response with high activity in the central area. In the acute hepatitis and chronic hepatitis, liver G6PD activity increased and ICD activity decreased. In livers at the active stage of chronic hepatitis, G6PD activity increased and ICD activity decreased remarkably in the periportal area where piecemeal necrosis was observed. In liver cirrhosis some areas of hyperplastic nodule showed almost normal G6PD and ICD activity, while in internodular areas increased G6PD and decreased ICD activity were detected. In liver stroma in cirrhosis high G6PD activity and low ICD activity were detected.View full abstractDownload PDF (2270K) -
MASAO OHYUMI1976 Volume 9 Issue 1 Pages 37-38
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (135K) -
S. SENO, N. OTSUKA, NAGAYASU OTSUKA, SHIGETO KANDA, JUNZO SASAKI, T. O ...1976 Volume 9 Issue 1 Pages 39
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (133K) -
S. SENO, N. OTSUKA, NAGAYASU OTSUKA, SHIGETO KANDA, JUNZO SASAKI1976 Volume 9 Issue 1 Pages 39a-45
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (6515K) -
T. OHKURA1976 Volume 9 Issue 1 Pages 46-51
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (3524K) -
AKIRA KOBATA, TAKASHI MURAMATSU, SHUN-ICHIRO OGATA, SEIICHI TAKASAKI1976 Volume 9 Issue 1 Pages 51-52
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (287K) -
NOBUTAKA ITO1976 Volume 9 Issue 1 Pages 53-54
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (307K) -
TAKAMITSU SEKINE, KIMIYO A. KATO1976 Volume 9 Issue 1 Pages 54-58
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSThe information has been accumulated on the participation of cell and organella membranes to biological processes such as hormonal integration, active transport, nerve impulse propagation and energy transduction. Application of fluorescence probes to obtain a better understanding of the structure and dynamics of membrane has already provided useful information, but is still in its infancy, partly due to our insufficient knowledge on the whole processes in a fluorescent molecule taking place during its excited state.View full abstractDownload PDF (714K) -
KOZO UTSUMI, MASANOBU MIYAHARA, KATSUMI SUGIYAMA, JUNZO SASAKI1976 Volume 9 Issue 1 Pages 59-68
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSSince the fluid mosaic model for the biological membrane has been proposed by Singer and Nicolson (21), many investigators have reported that the membrane fluidity is closely related to the various metabolic functions of the cell. The present investigation was performed to examine that a biscoclaurin alkaloid, cepharanthin which showed protective effects from the snake venom action might modify the fluidity to various cell membranes modulated by various agents. The obtained results were as follows.
1. Cepharanthin inhibits K+ release from red blood cells, Ehrlich ascites tumor cells, AH-130 ascites hepatoma cells and liposome when these were treated with snake venom, phospholipase A, lysolecithin and lead acetate. But cepharanthin does not inhibit K+ release induced by ionophores, Triton X-100 and HVJ.
2. Cepharanthin inhibits the release of histamine from rat peritoneal mast cells induced by various releasers.
3. The alkaloid inhibits cap formation of rat monocytes and Ehrlich ascites tumor cells.
4. Fluorescence polarization analyses and spin-label studies indicated that cepharanthin decreased fluidity of various biological membranes.
5. From these results it is concluded that cepharanthin decreases membrane fluidity and acts as a potent membrane stabilizing agent.View full abstractDownload PDF (1717K) -
VINCI MIZUHIRA1976 Volume 9 Issue 1 Pages 69-87
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (10322K) -
Fumie SASAKI, Shiro NAKAGAWA, Chiharu SUEMATSU, Tajimi HIROHATA, Eiich ...1976 Volume 9 Issue 1 Pages 88-104
Published: 1976
Released on J-STAGE: February 26, 2010
JOURNAL FREE ACCESSDownload PDF (3066K)
- |<
- <
- 1
- >
- >|