ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
9 巻, 4 号
選択された号の論文の7件中1~7を表示しています
  • A. K. RAI, A. K. MITTAL
    1976 年 9 巻 4 号 p. 269-278
    発行日: 1976年
    公開日: 2009/10/28
    ジャーナル フリー
    The localization and characterization of proteins and nucleic acids in the various cellular constituents of the skin of Heteropneustes fossilis have been described using several histochemical techniques.
    The basal cells give weak reactions for protein bound NH2 groups, basic nucleoproteins and tyrosine. The contents of the giant cells are proteineous in nature and give moderate reaction for protein bound NH2 groups, basic proteins, tyrosine and mucoprotein. The perinuclear areas of the giant cells, however, remain almost unstained. The mucous cells remain almost unstained with the various tests employed for proteins. The polygonal cells in the middle and outermost layer give positive reactions for protein bound NH2 groups, basic proteins, basic nucleoproteins, arginine, tyrosine and nucleoprotein. These cells even in the most superficial layer fail to give positive reactions for cysteine bound sulphydrly groups (-SH), disulphide bonds of cystine and Papanicolaou's stain for keratin indicating the non keratinized nature of the epidermis.
    In addition to the basal layer, the lymphocytes have also been observed in the middle layer and the outermost layer. This has been correlated to increase the efficiency in the local defence mechanism of the fish.
    A thin layer of compactly arranged elastic fibres at the inner margin of the dermis has been correlated to provide elasticity to the skin, thus facilitating the easy movements of the fish at acute angle.
  • HAJIME AOE, KAZUHISA TAKETA, MASATOSHI UEDA, AKIRA TANAKA, KIYOWO KOSA ...
    1976 年 9 巻 4 号 p. 279-285
    発行日: 1976年
    公開日: 2009/10/28
    ジャーナル フリー
    The activities of fructose 1, 6-diphosphatase in hepatocellular carcinoma tissues were studied histochemically. The formazan deposition, which represents the activity of fructose 1, 6-diphosphatase, was found to be localized in the cytoplasm of hepatoma cells. The degree of the formazan deposition was proportional to the activity obtained by a kinetic method with tissue extracts.
    The three hepatoma cases studied were all well differentiated hepatocellular carcinoma and had varying FDPase activities, ranging from near normal to undetectable levels, even though their degrees of histochemical differentiation were quite similar.
  • KANJI KISHI, KATSUMI NISHIJIMA, HERMAN MEDAK, PAUL BURLAKOW
    1976 年 9 巻 4 号 p. 286-291
    発行日: 1976年
    公開日: 2009/10/28
    ジャーナル フリー
    Cytochemical studies of acid phosphatase activity of oral mucosa cells have been carried on the frozen-dried smears employing the method of standard naphthol AS phosphate devised by Burstone. The purpose is to observe the possible interrelation between the intensity of acid phosphatase activity and some pathologic changes. The specimens were obtained from various areas of oral cavity of 71 persons. In the oral mucosa from healthy individuals, acid phosphatase activity was found to be positive in all the cells from various areas. Intense acid phosphatase reaction was seen in the cells of palate and the gingiva. Activity of the enzyme decreased in inflammatory conditions. The mechanism of enhancement of acid phosphatase activity in inflammatory condition was discussed in relation to keratinization.
  • TRICHOPHYTON MENTAGROPHYTES CELL WALL
    EIKO SHINJI, YUJI SHINJI, VINCI MIZUHIRA
    1976 年 9 巻 4 号 p. 292-305
    発行日: 1976年
    公開日: 2009/10/28
    ジャーナル フリー
    Whichever sections of Trichophyton mentagrophytes were treated by chromic acid-silver methenamine, periodic acid-silver methenamine, periodic acid-thiocarbohydrazide-silver proteinate, or alkaline bismuth histochemical staining techniques, the cell walls were densely impregnated and clearly defined.
    In the case of sections stained with alkaline bismuth, the cell walls revealed histochemical ultrastructures, there being fine granular structures all over, microfibrous structures in the inner half of the cell wall, and coarse granular structures arranged close together on the outer surface of the plasma membrane.
    It was discovered from the results of controlled experiments involving acetylation, saponification, periodic acid oxidation and chitinase digestion that the chemical substances of the afore-mentioned structures are in the main composed of polysaccharides containing vic-glycol groups, and supported by chitin. Furthermore, satisfactory electron micrographs of the general structures of fungal cells were obtained from sections which had been treated with alkaline bismuth, combined with uranyl acetate, followed by the lead citrate poststaining technique.
  • TAKURO SUZUKI, MASARU KIMURA, KAZUTO NOKUBI, TAKESHI MURAKI, MORIO KAT ...
    1976 年 9 巻 4 号 p. 306-314
    発行日: 1976年
    公開日: 2009/10/28
    ジャーナル フリー
    An addition of methanol into the aqueous solution of Chrome azurol S (CAS) accelerates the reaction of CAS with Cr. Mechanism of the accelerative effect of methanol was discussed spectrometrically. CAS has four chemical species, and the third chemical species (in which three protons released) is most liable to chelate Cr. In a high pH range, the third chemical species increases in the solution, however, at the same time, an increase in hydroxy ion interrupts the reaction between CAS and Cr. Results of spectrometrical measurements indicate that the added methanol accelerates the dissociation of the second chemical species of CAS in a low pH range and the reduction of the fourth chemical species in a high pH range. Consequently, the third chemical species is highly concentrated in the solution, that leads to accelerating the Cr-CAS reaction without any interruption caused on an increase of hydroxy ion.
  • KIMINAO MIZUKAWA, KEISUKE SHIMIZU, TADAO MATSUURA, YASUHIKO IBATA, YUT ...
    1976 年 9 巻 4 号 p. 315-322
    発行日: 1976年
    公開日: 2009/10/28
    ジャーナル フリー
    The degenerative changes in the floor portion of the third ventricle of the rats were investigated by fluorescence histochemistry and electron microscopy after administration of an anthelmintic, kainic acid (10-40mg/kg).
    Catecholamine fluorescence in the arcuate nucleus and the external layer of the median eminence was markedly depleted and almost disappeared 2hr after administration. Light microscopically, the nerve cells of the arcuate nucleus showed strong vacuolization and pyknotic changes simultaneously. Electron microscopically, the cytoplasmic degenerative changes and massively dilated dendritic profiles were observed in the arcuate nucleus. The dense cored vesicles were decreased in number and electron dense bodies and myelin figures appeared in the nerve endings of the external layer of the median eminence.
  • FUSAYOSHI MURATA, SHINICHI OHNO, YURIKO MOMOSE, TETSUJI NAGATA
    1976 年 9 巻 4 号 p. 323-331
    発行日: 1976年
    公開日: 2009/10/28
    ジャーナル フリー
    A simplified method for mass production of electron microscopic specimens for histochemistry without using any special instrument is introduced. This is also useful for a very small amounts of tissues. The method prevents the loss of thick sections during the treatment and it does not produce any nonspecific staining. The wide future application is expected.
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