Peroxidase-like activities of glass-beads modified with metal-porphines (M-P
G) were examined through a dye-formation reaction, which was catalyzed by peroxidase, of phenol and 4-aminoantipyrine in the presence of hydrogen peroxide. When M-P
G was used as a catalyst in place of peroxidase, glass-beads modified with Mn
3+- and Fe
3+-tetrakis(4-carboxyphenyl)porphine derivatives (Mn- and Fe-TCPP
G) showed relatively strong peroxidase-like activity among the M-P
G examined. The percentage of the resulting quinoid dye by Mn- and Fe-TCPP
G reached to about 93% and 88%, respectively, of that formed by peroxidase. Peroxidase-like activity of Mn-TCPP
G was applied to the determination of hydrogen peroxide in place of peroxidase. Furthermore, the determination of serum compounds (glucose and uric acid), which form hydrogen peroxide by using their oxidases, was examined.
View full abstract