Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
19 巻, 8 号
選択された号の論文の30件中1~30を表示しています
Rapid Communications
  • Kazunori NAKASHIMA, Fukiko KUBOTA, Tatsuo MARUYAMA, Masahiro GOTO
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1097-1098
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    Octyl(phenyl)-N,N-diisobutylcarbamoylmethyl phosphine oxide (CMPO) dissolved in an ionic liquids, 1-butyl-3-methylimidazolium hexafluorophosphate, greatly enhances extractability and selectivity of lanthanide cations compared to that dissolved in conventional organic solvents; further, the recovery of lanthanides extracted into ionic liquids can be accomplished using several stripping solutions containing complexing agents. The possibility of utilizing ionic liquids as novel separation media in an industrial liquid-liquid extraction process was demonstrated.
Original Papers
  • Jun LIU, Qingwen LI, Yadong YU, Xiang FANG
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1099-1102
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    Dopamine may show some biological activities in antitumor and cell apoptosis. Herein, we attempted to employ UV-Vis, CD, and electrochemical methods to investigate the interaction between DNA and dopamine. Both the spectroscopic and electrochemical evidence indicated that dopamine, which was a cation, could interact with polyanion DNA. However, this kind of interaction, mainly by a static electronic force, did not result in any damage of the DNA structure. This situation was quite different when Cu ion was added to the dopamine-DNA system; an obvious change in the color of the solution and in the spectra of UV-Vis and CD showed that some chemical changes occurred in this system, and that the DNA native structure was destroyed. The results of gel electrophores further revealed that DNA might be broken into small segments by dopamine in the presence of Cu ion. The possible reaction mechanisms are discussed.
  • Sachiko SUZUKI, Ken-ichi OHNO, Tomofumi SANTA, Kazuhiro IMAI
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1103-1108
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    In this study, we developed a rapid, simple and homogeneous human recombinant estrogen receptor-β(hrER-β) binding assay method using fluorescence polarization (FP) by applying a fluorescent ligand, fluorescein-labeled estradiol (F-E2). A Scatchard plot and a Hill plot analysis of the saturation binding assay using F-E2 and hrER-β were studied. F-E2 showed a high affinity for hrER-β, the dissociation constant was 5.53 nM, indicating that F-E2 is applicable to the hrER-β binding assay. Competitive binding assays using F-E2, in which the FP values decreased upon the addition of compounds (competitors) were carried out to evaluate the binding characteristics of compounds with and without biological activities to hrER-β. Twenty-one compounds, such as hormones, pharmaceuticals, industrial chemicals and phytoestrogens, were examined. The obtained sigmoidal inhibition curves were transformed into pseudo-Hill plots and the concentrations at 50% inhibition (IC50) and Hill coefficients, the degree of cooperativity in ER-ligand binding, were obtained from the regression equations. Antagonists exhibited larger Hill coefficients than agonists, showing the correlation between the Hill coefficients and the estrogenic/antiestrogenic activities.
  • Kenichi TONOKURA, Tomohisa NAKAMURA, Mitsuo KOSHI
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1109-1113
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    Vacuum ultraviolet single-photon ionization time-of-flight mass spectrometry (VUV-SPI-TOFMS) has been applied for the detection of chlorobenzene, o-dichlorobenzene, and o-chlorophenol as surrogates for polychlorinated dibenzo-p-dioxine/furans (PCDD/F). The photoionization mass spectra of these compounds appear to be fragmentation free in the ionization processes by the VUV-SPI at 10.2 eV (121.6 nm). Quantum chemical calculations support no fragmentation in the photoionization of chlorobenzene derivatives at around 10 eV. The absolute photoionization cross-sections of chlorobenzene, o-dichlorobenzene, and o-chlorophenol were estimated at 10.2 eV. The photoionization cross-section is an important parameter in the detection of chlorobenzene derivatives by the single-photon ionization technique. The detection limit for chlorobenzene is on the order of tenth parts-per-billion volume (ppbv) in the present experimental setup.
  • Y. M. TEMERK, M. M. KAMAL, G. A-W. AHMED, H. S. M. IBRAHIM
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1115-1119
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    The surface activity of xanthine (Xan) and xanthosine (Xano) at a hanging mercury drop electrode (HMDE) was studied using out-of-phase ac and cyclic dc voltammetry. The results show that Xan and Xano were strongly adsorbed and chemically interacted with the charged mercury surface, which is the prerequisite step for applying the cathodic adsorptive stripping voltammetric determination of such biologically important compounds. Differential pulse cathodic adsorptive stripping voltammetry (DPCASV) and square-wave cathodic adsorptive stripping voltammetry (SWCASV) were applied for the ultratrace determination of Xan and Xano compounds. Moreover, a rapid and sensitive controlled adsorptive accumulation of Cu(II) complexes of both compounds provided the basis of a direct stripping voltammetric determination of such compounds to submicromolar and nanomolar levels. Operational and solution conditions for the quantitative ultratrace determination of Xan and Xano were optimized in absence and presence of Cu(II). The calibration curve data were subjected to least-squares refinements. The effects of several types of inorganic and organic interfering species on the determination of Xan or Xano were considered.
  • Alireza ASGHARI, Mohammad K. AMINI, Hassan R. MANSOUR, Masood SALAVATI ...
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1121-1125
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    A nitrate-selective electrode based on a recently synthesized bis(2-hydroxyanil)acetylacetone lead(II) complex [(haacac)Pb] has been developed. Among different compositions studied, a membrane containing 30.7% poly(vinyl chloride) (PVC), 61.3% dibutyl phthalate (DBP) as a plasticizer, 3% methyltrioctylammonium chloride (MTOAC) as a cationic additive and 5% ionophore (all w/w) exhibited the best potentiometric response toward nitrate ion in aqueous solutions. The potentiometric response of the electrode was linear with a Nernstian slope of -58.8 mV decade-1 within the NO3- concentration range of 2 × 10-5 - 1 × 10-1 mol dm-3. The response time of the electrode was ≤ 10 s over the entire linear concentration range of the calibration plot. The electrode is suitable for use within the pH range of 5.3 - 11. The selectivity coefficients for the proposed electrode were improved for some interferences, when compared with those of commercially available nitrate-selective electrodes.
  • Mohammad REZA GANJALI, Morteza REZAPOUR, Mohammad REZA POURJAVID, Maso ...
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1127-1131
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    A cobalt(II) derivative was used as a suitable ionophore for the preparation of a polymeric membrane nitrite-selective electrode. The electrode reveals a Nernstian behavior over a very wide NO2- ion concentration range (1.0 × 10-6 - 1.0 × 10-1 M) and a very low detection limit (5.0 × 10-7 M). The potentiometric response is independent of the pH of solution in the pH range 4.0 - 9.5. The electrode shows advantages such as low resistance, fast response and, most importantly, good selectivity relative to a wide variety of inorganic and organic anions. In fact, the selectivity behavior of the proposed NO2- ion-selective electrode shows great improvements compared to the previously reported electrodes for nitrite ion. The proposed electrodes could be used for at least 2 months without any significant changes in potentials. The electrode was successfully applied to the determination of nitrate ion concentrations in sausage and milk samples.
  • Koichi YAMAMOTO, Kana ADACHI
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1133-1138
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    A spectrophotometric method for the determination of ionic surfactants with Bromophenol Blue (BPB) based on incorporation into a precipitated chitosan was studied. Cationic surfactants (CS+), such as a quaternary ammonium ion containing a long-chain alkyl group, associate with BPB2- buffered at about pH 9 to form the ion associate (CS+)2·BPB2-. CS+ associates with anionic surfactants (AS-). In the presence of a definite amount of CS+, an increase in the amount of AS- leads to a decrease in the amount of excess CS+, and therefore to a decrease in the amount of the ion associate of CS+ with BPB2-. The addition of a chitosan dissolved in acetic acid to a solution containing these ion associates leads immediately to precipitation of the chitosan and the incorporation of the ion associates (CS+)2·BPB2- or CS+·AS- into the precipitated chitosan. After centrifuging, ionic surfactants can be determined by the following two methods: (1) the absorbance of the supernatant solution is measured at 590 nm. (2) After the supernatant solution is separated, the precipitate is dissolved in an acetic acid solution and the absorbance is measured at 625 nm. Because the color of the precipitate is judged by the naked eye, this can be applied to the visual method. This is a simple and rapid method for the determination of a 10-6 M order of ionic surfactants.
  • José Arnaldo Dibbern FÁVERO, Matthieu TUBINO
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1139-1143
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    A selective, sensitive, rapid and simple-handling analytical method for the determination of cyanide at low detection limits in surface and underground water, soil and industrial waste samples was developed. The method is based on a reaction, proposed by Guilbault and Kramer, where free cyanide reacts with p-nitrobenzaldehyde to form an intermediate cyanohydrin, which reacts with o-dinitrobenzene to give a highly colored purple compound. The original procedure was modified for application in a small device containing a gas-permeable membrane. The cyanide is converted in the volatile hydrogen cyanide, which permeates through a PTFE membrane, reaching colorimetric reagents. In order to obtain semi-quantitative results, printed color scales were built. The method allows rapid, accurate, selective, low-cost and simple-handling determinations of free cyanide, even in complex samples. About 150 real samples were analyzed. Less than 10 ng of free cyanide per ml (10 μg l-1) can be easily detected. For more concentrated solutions, the results had been compared to those obtained using differential pulse polarography. The standard addition method was used for more diluted solutions.
  • Nawal A. A. ALARFAJ
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1145-1149
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    A chemiluminescence (CL) method using flow injection (FI) has been investigated for the rapid and sensitive determination of enalapril maleate. The method is based on the CL reaction of the drug with tris(2,2′-bipyridyl)ruthenium(II), Ru(bipy)32+ and acidic potassium permanganate. After selecting the best operating parameters, calibration graphs were obtained over concentration ranges of 0.005 - 0.2 μg/ml and 0.7 - 100 μg/ml with a detection limit (S/N = 2) of 1.0 ng/ml. The average % found was 99.9 ± 0.7 and 100.2 ± 0.3 for the two concentration ranges respectively. %RSD (n = 10) for 5.0 μg/ml was 0.44. The method was successfully applied to the determination of enalapril maleate in dosage forms and biological fluids without interferences.
  • Gamal A. E. MOSTAFA, Mohamed M. HASSANIEN, Khaled S. ABOU-EL-SHERBINI, ...
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1151-1156
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    Controlled-pore silica glass modified with N-propylsalicylaldimine (SCPSG) has been investigated as a surface-active matrix for the separation of some metal ions. The porous silica glass base was confirmed to have better stability towards hydrolysis in aqueous solution buffered at pH = 9 in comparison to silica gel, which showed twice the surface area of controlled-pore silica glass. The different analytical parameters affecting the batch mode separation and preconcentration of trace Al(III), Ag(I) and Hg(II) in environmental samples using SCPSG, prior to their determination using inductively coupled plasma mass spectrometry (ICP-MS), were studied. The optimum conditions are pH 9.0 &plesmn; 0.1, time of stirring 30 min and the eluent concentration 0.5 mol dm-3 HNO3. The ion-exchange capacity of SCPSG with respect to Al(III), Ag(I) and Hg(II) was 0.27, 0.18 and 0.23 mmol g-1, respectively. The recovery values for the metal ions were 96.8 ± 0.86, 98.1 ± 0.60 and 96.2 ± 1.06%, and the analytical detection limits were 26.1, 1.49 and 0.44 pg cm-3, respectively, for a preconcentration factor of 100. The method has been applied to the determination of the investigated metal ions in natural water samples as well as certified and reported samples and the results were found to be accurate.
  • Guo-Sheng YANG, Piedad Parrilla VAZQUEZ, Antonia Garrido FRENICH, Jose ...
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1157-1161
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    The enantiomers of fourteen O,O-dialkyl-2-benzyloxycarbonyl-aminoarylmethyl-phosphonates are directly separated on the tris(3,5-dimethylphenylcarbamate) cellulose column (Chiralcel OD-R) under reversed phase mode. The results of the chiral separation are different from the results obtained in the normal phase mode. The mobile phase plays an essential role in chiral discrimination when using Chiralcel OD-R. The influences of the mobile phase composition on the retention and the enantioselectivity are investigated. The influences on chiral separation of the length and steric hindrance of alkoxy groups of the phosphonate ester and of the nature of the substituent on the benzene ring that is attached to the chiral carbon atom are also discussed.
  • Mingyu DING, Shuaiwu MA, Delin LIU
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1163-1165
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    A simple and reliable high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of five hydroxyanthraquinones (aloe-emodin, rhein, emodin, chrysophanol, and physcion) in Rhubarb and experimental animal bodies. A Zorbax SB-C18 column (250 mm × 4.6 mm i.d., 5 μm) and a methanol -0.5% acetic acid (85:15, v/v) mobile phase were used for the separation. The detection wavelength of a diode array detector (DAD) was set at 254 nm. Regression equations revealed a linear relationship (R2 > 0.9996) between the mass of hydroxyanthraquinones injected and the peak areas detected by DAD. The detection limits (S/N = 3) ranged from 0.35 ng to 3.13 ng, and the recoveries ranged from 83% to 103% for different hydroxyanthraquinones. This method is simple, sensitive and suitable for the analysis of hydroxyanthraquinones in medicinal materials and pharmacological experiment samples.
  • Pei LIANG, Lanhao YANG, Bin HU, Zucheng JIANG
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1167-1171
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    Titanium dioxide nanoparticle dynamically loaded with 8-hydroxyquinoline (nanometer TiO2-Oxine) was used as a solidphase extractant for the preconcentration of trace amounts of aluminum(III) and chromium(III) prior to their determination by inductively coupled plasma atomic emission spectrometry (ICP-AES). The optimal conditions for preparing nanometer TiO2-Oxine were obtained. Also, the separation/preconcentration conditions of analytes, including the effects of the pH, the sample flow rate and the volume, the elution solution and the interfering ions on the recovery of the analytes were investigated. At pH 6.0, the adsorption capacity of nanometer TiO2-Oxine was found to be 5.23 mg g-1 and 9.58 mg g-1 for Al(III) and Cr(III), respectively. An enrichment factor of 50 was achieved by this method, and the detection limits (3σ) for Al(III) and Cr(III) were 1.96 and 0.32 μg L-1 respectively. The proposed method was applied for the determination of trace Al(III) and Cr(III) in biological samples and lake water with satisfactory results.
  • Shulin ZHAO, Dianmei LIU, Shuting LI
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1173-1176
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    A novel spectrophotometric method has been developed for the determination of proteins using the p-acetychlorophosphonazo (CPA-pA)-barium(II) complex as a spectroprobe. In the pH range of 2.0 - 2.8, the absorbance of the CPA-pA-barium(II) complex at 649 nm is greatly decreased by protein. The absorbance decrease is in proportion to the concentration of protein in the range of 0 - 20 mg/L. The apparent molar absorptivities are 1.56 × 106, 1.70 × 106 and 6.04 × 105 L mol-1 cm-1 for bovine serum albumin (BSA), human serum albumin (HAS) and ovalbumin (OVA), respectively. The method is reproducible and simple, and has been used to determine total protein in human sera. The results are in agreement with those obtained by the pyrocatechol violet (PV)-Mo(VI) method, with relative standard deviations of 2.5 - 3.5% (n = 6).
  • Akio YUCHI, Hiroaki HIRAMATSU, Miyuki OHARA, Nayumi OHATA
    原稿種別: scientific monograph
    専門分野: Infomation Science
    2003 年 19 巻 8 号 p. 1177-1181
    発行日: 2003年
    公開日: 2004/02/27
    ジャーナル フリー
    An X-ray crystallographic study demonstrated that the tris complex of Al3+ with 2-methyl-8-quinolinol (Hmq), [Al(mq)3], had a discrete octahedral geometry in a meridional configuration with respect to mq- the Al-N bonds were appreciably elongated. In organic solvents, the complex reacted with water to give a lower species of a μ-oxo dimer, [Al2O(mq)4], and a protonated ligand, depending on the concentrations of water and of excess ligand. None of Cl- Br- I- ClO4-, and CH3COO- showed any reactions with [Al(mq)3]. The anions having high affinities to Al3+, such as H2PO4- and F- replaced all the ligand. The anion having a lower affinity, HSO4-, exclusively formed a mixed-ligand complex of [Al(mq)2HSO4], which had a more intense fluorescence than the others. In contrast, the corresponding 8-quinolinol (Hq) complex, [Alq3], showed no reactions with any anions.
Notes
Instrumental Achievements
feedback
Top