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Hiroshi HASHITANI
1989 年 5 巻 5 号 p.
495
発行日: 1989年
公開日: 2007/07/27
ジャーナル
フリー
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Akio TSUJI, Masako MAEDA, Hidetoshi ARAKAWA
1989 年 5 巻 5 号 p.
497-506
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
Highly sensitive chemiluminescent enzyme immunoassays developed in our laboratory are reviewed. Oxidases, peroxidase and glucose oxidase, generating H
2O
2 can be assayed by isoluminol/microperoxidase or peroxyoxalate/fluorescent dye. β-D-Galactosidase is also assayed by chemiluminescence reaction based on the coupled enzyme reaction using lactose/glucose oxidase. β-D-Galactosidase can be measured by using o-nitrophenyl β-D-galactoside as substrate and chemiluminescence reaction of NADH after coupled enzyme reaction of galactose dehydrogenase. Sucrose and lucigenin are used as substrate and chemiluminescent reagent for the assay of invertase. Glucose-6-phosphate is determined by using glucose 6-phosphate as substrate and chemiluminescent assay of NADH. Alkaline phosphatase is assayed by using NADP
+, alcohol dehydrogenase and hemiluminescence reaction of NADH. These methods can be applied successfully to the chemiluminescent enzyme immunoassays for various hormones and drugs in biological fluids.
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Yusuke OKAWA, Hirohiko TSUZUKI, Shoichiro YOSHIDA, Tadashi WATANABE
1989 年 5 巻 5 号 p.
507-512
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
Glucose oxidase was covalently immobilized, as a monomolecular layer, onto a tin(IV) oxide (SnO
2) electrode via (1) 2, 4, 6-trichoro-1, 3, 5-triazine or (2) 3-aminopropyltriethoxysilane and glutaraldehyde, to give an amperometric glucose sensor. The sensor thus obtained exhibited an order-of-magnitude higher specific sensitivity (i.e. sensitivity per immobilized enzyme molecule) and, even without continuous oxygen supply, a higher upper detection limit than a conventional type sensor carrying a crosslinked glucose oxidase membrane. The sensors showed a long-term stability (>1 month). When this sensor was applied to a flow injection glucose analyzer system, it showed a wide dynamic range (10
-5-10
-1M) and high analysis frequency (15 samples h
-1 at a flow rate of 0.5ml min
-1). The sensor was also used as a detector in a high performance liquid chromatograph system. The amount of glucose in a control serum was determined, in good agreement with the value determined by conventional methods.
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Shinzo TANABE, Tadayasu TOGAWA, Koji KAWANABE
1989 年 5 巻 5 号 p.
513-516
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A simple and rapid flow injection method has been developed for the colorimetric determination of resorcinol-type β
2-adrenergic drugs: fenoterol hydrobromide, orciprenaline sulfate and terbutaline sulfate, by using phenanthro-[9, 10-
d]imidazole-2-
N-chloroimide (Pl-NCl) as a color reagent. The color reaction between the drugs and Pl-NCl occurs at alkaline pH. Under the optimized conditions, the calibration curves for the three drugs were linear over the range of 0.5-200μM. The relative standard deviations (
n=10) for fenoterol hydrobromide, orciprenaline sulfate and terbutaline sulfate were 1.7, 1.4 and 1.0%. The present method was applied to the determination of those drugs in commercial formulations.
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Seiki YAMAGUCHI, Osamu TAKEMURA, Tokuji IKEDA, Mitsugi SENDA
1989 年 5 巻 5 号 p.
517-519
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
This assay for superoxide dismutase (SOD) involves two electrode reactions: 1) the generation of a substrate (O
2-) by a one-electron reduction of dissolved oxygen at a constant potential of -1.0V in a pH 11.0 borate-carbonate buffer solution containing 2×10
-4mol/l EDTA and 2.4×10
-4mol/l triphenylphosphine oxide, and 2) the detection of a residual substrate, which results from an SOD-catalyzed reaction at -0.1V by one-electron oxidation with anodic scan pulse voltammetry. By the use of a hanging mercury drop electrode (a static mercury drop electrode) and with a sequence-control technique, these reactions are so strictly controlled that an SOD as low as 10
-12mol/l can be determined within a short time (<30s) under automatic operation.
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Hiroshi NAKAMURA, Tadao KONISHI, Masafumi KAMADA
1989 年 5 巻 5 号 p.
521-524
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
Three commercial gradient systems were compared to clarify the problems associated with gradient elution. The precision of the gradient elution was evaluated as the reproducibilities (RSD) of retention times of aromatic compounds and proteins separated on a reversed phase column. Actual gradient elution curves differ considerably from each other among the three systems tested, which affected the resultant chromatograms. Furthermore, RSD values for proteins were larger than those of aromatic compounds for all three systems; the retention of proteins was sensitive to a slight change of the resultant gradient elution pattern. Based on these findings, a method is presented for the evaluation of gradient systems by using proteins as sensitive markers.
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Hiroaki NAGAOKA, Hitoshi NOHTA, Yosuke UHKURA, Yumi KAETSU, Mikihiko S ...
1989 年 5 巻 5 号 p.
525-530
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
2-(5-Chlorocarbonyl-2-oxazolyl)-5, 6-methylenedioxybenzofuran was found to be a highly sensitive fluorescence derivatization reagent for primary, secondary and tertiary alcohols in high performance liquid chromatography. Its reactivity was investigated for 1-hexanol, benzyl alcohol, cyclohexanol and 2-methyl-2-butanol. The reagent reacts with the alcohols in benzene in the presence of pyridine to produce the corresponding fluorescent esters, which could be separated on a reversed phase column, Cosmosil 5C
18, with aqueous 70% (v/v) acetonitrile; the detection limits (
S/N=3) were 3-4fmol for 1-hexanol and benzyl alcohol, 12fmol for cyclohexanol and 900fmol for 2-methyl-2-butanol for an injection volume of 20μl. Hydroxycarboxylic acids did not give fluorescent derivatives under these conditions.
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Yutaka HAYASHIBE, Fumiko WATAI, Yukari ENOKI, Koichi OGUMA, Rokuro KUR ...
1989 年 5 巻 5 号 p.
531-533
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A combined ion-exchange-graphite furnace atomic absorption spectrometric method has been developed for the determination of ultra traces of beryllium in biological materials. Beryllium is enriched in a cation-exchange column from an oxalic acid-hydrochloric acid medium and recovered by elution with sulfosalicylate (pH7). Eluted beryllium is purified in the same salicylate medium simply by anion-exchange, followed by quantification with graphite furnace atomic absorption spectrometry without a matrix modifier. The method is microchemical, allowing beryllium in the ng/g levels to be determined with a relative standard deviation of 5% on a 200mg sample basis. Results are quoted for several biological reference materials of NIST (USA) and NIES (Japan).
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Tadashi MOCHIZUKI, Akiko SAKASHITA, Takanori AKIYOSHI, Hideo IWATA
1989 年 5 巻 5 号 p.
535-538
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A laser ablation system using a cw Q-switched Nd: YAG laser has been developed for the direct analysis of steels by inductively coupled plasma emission spectrometry (lCP-AES). The YAG laser used for this work produced fine particles (particle size, <0.2μm). The amount of particles obtained by ablating pure iron for 20s was about 400μg. The ablated samples were directly introduced into the plasma. The horizontal movement of sample during laser firing prevented the laser beam from becoming out of focus on the sample surfaces, and that was effective to improve sensitivity and precision. Variation in the amount of the particles ablated at each analysis impaired analytical precision; consequently, the matrix element of iron was used as the internal standard. The precision(RSDs, %) values for seven elements in steel were 0.3-1.6%. Detection limits ranged from 2ppm for Ni, Cr and Cu to 87ppm for Si.
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Kuniyuki KITAGAWA, Akihiko MIZUTANI, Masaaki YANAGISAWA
1989 年 5 巻 5 号 p.
539-544
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A separative column atomizer (SCA) is attached to a helium microwave-induced plasma (MlP) at atmospheric pressure. The joint use is intended for of trace amounts of elements in complex matrices by atomic emission spectrometry. Basic characteristics of the constructed SCA-MIP are studied for the test lements, Hg and Br in various matrices.
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Kenji KATO, Yoshinori SUGITANI
1989 年 5 巻 5 号 p.
545-548
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A simultaneous measurement of photoacoustic and fluorescence signals has been attempted using an X-ray fluorescence spectrometer equipped with a photoacoustic cell designed for the present purpose. The combined measurement was conducted for metal foils and model samples with layered structures. The fluorescence measurement gave information concerning the elements of the samples and the photoacoustic signal gave information concerning the total amount of X-ray absorption and the thermal properties of the samples. The same samples were also examined through ordinary photoacoustic measurements by visible light. The use of different excitation wavelengths in the combined measurement was found to give more detailed information about the thermal and structural properties than the individual use of these techniques.
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Fermin CAPITAN, Eloisa MANZANO, Jose Luis VILCHEZ, Luis Fermin CAPITAN ...
1989 年 5 巻 5 号 p.
549-555
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
Morin (2′, 3, 4′, 5, 7-pentahydroxyflavone) has been used as a ligand in the fluorometric microdetermination of aluminum at the sub-μgl
-1 level. A method has been developed which is based on ion exchanger fluorometry (IEF). The 1:1 aluminum-morin complex is fixed on a dextran-type cationic exchanger, and the fluorescence of the gel, packed on a 1-mm quartz cell, is measured directly using a solid-surface attachment. The concentration for the method ranges between 0.4 and 1.6μgl
-1, although this can be diminished by increasing the sample volume. The method has been successfully applied to the determination of aluminum in natural water and offers several advantages in comparison with solution methods.
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Kurt SEILER, Werner E. MORF, Bruno RUSTERHOLZ, Wilhelm SIMON
1989 年 5 巻 5 号 p.
557-561
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A novel principle of an optical sensor (optode) has been realized in practice by combining NH
4+-selective neutral ionophores of the macrotetrolide type, H
+-selective neutral chromoionophores that drastically change their absorption spectrum upon protonation, and lipophilic anionic sites in the same plasticized PVC membrane. Such chemical sensors generally offer the advantages of a highly selective and reversible recognition of given ionic substrates and of a straightforward optical transduction of the recognition process. The present membrane exhibits the theoretically expected absorbance response to ammonium ions in pH-buffered samples. Dynamic range, selectivity coefficients, reproducibilities, and response times of the new optode system are promising in view of analytically relevant applications.
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Zhiqiang GAO, Xuezhi SI, Zaofan ZHAO
1989 年 5 巻 5 号 p.
563-566
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
In a 0.3M sodium hydroxide medium there is a well-defined single-sweep polarographic wave for a Cd(II)-3-hydroxy-1-
p-sulfonatophenyl-3-phenyl triazene complex which can be used for the determination of trace amounts of cadmium. The complex, corresponding to Cd(HSPT)
2 with a conditional formation constant log β′=9.20, can be strongly adsorbed on the surface of a mercury electrode; the polarographic wave is attributed to the reduction of Cd(II) in the adsorbed complex to Cd(Hg). The linear range for the polarographic wave is from 1×10
-8 to 1×10
-6 M and the detection limit is 6×10
-9M. The relative standard deviation is 1.5% and the regression correlation coefficient is 0.998. Most common metal ions show no, or little, effect on the determination. This method has been applied for the analysis of waste water and biological samples.
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Daisuke YOSHIZAWA, Kunio SHIMIZU, Gen P. SATO, Konosuke KUMAKURA, Koic ...
1989 年 5 巻 5 号 p.
567-570
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
The response of a flow injection analysis with an electrochemical detector was made independent of the flow rate by using a singe bead string reactor tube (SBSR) and by operating the detector in the rapid-scan square-wave voltammetric mode. The SBSR reduced the flow-rate dependence of the maximum concentrations of the sample zone to 10% and the square-wave voltammetric peak height was virtually independent of the flow rate. The voltage scan was triggered at the point of the maximum concentration of the sample zone. The point was detected by monitoring the ECD current at a constant potential. The operation was controlled by a personal computer. The detection limits for [Fe(CN)
6]
4- and dopamine were
ca. 0.6nmol (=2×10
-5mol dm
-3×29mm
3). The use of square-wave voltammetry allowed for the simultaneous determination of more than one component.
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Kazuho INABA, Hidehiko AIKAWA, Tatsuya SEKINE
1989 年 5 巻 5 号 p.
571-575
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
The rate of solvent extraction of chromium(III) in aqueous perch orate solutions with four kinds of β-diketones into 4-methyl-2-pentanone was determined. It was found that the extraction was much quicker than that into non-polar solvents and the whole extraction was controlled by the reaction of the hydrated chromium(III) ion with a ligand in the aqueous phase. From the solvent extraction data, the rate constant for this complex formation in the aqueous phase was calculated. The values of the rate constants, thus obtained, for these β-diketones were about three orders higher than those for the formation of chromium(III) complexes with several other ligands in aqueous solutions mentioned in literature. The possible extraction mechanism in the system is discussed.
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Tohru SAITOH, Yoshiya KIMURA, Tamio KAMJDATE, Hiroto WATANABE, Kensaku ...
1989 年 5 巻 5 号 p.
577-581
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A dilute aqueous micellar solution of poly(oxyethylene)=4-nonylphenyl=ether with oxyethylene units of 7.5 (average, PONPE-7.5) was separated into two phases (aqueous and surfactant) at room temperature. The partition constants of thiazolylazo dyes and their metal chelates between the two phases were determined at 293K and an ionic strength of 0.1(NaClO
4). Though a linear relationship was obtained between the partition constants of the chelating reagents in the extraction with PONPE-7.5 and those in the ordinary extraction with octanol, such a relationship was not obtained for the chelates. This suggested that the properties of the PONPE-7.5 phase as an extracting solvent were different from those of ordinary organic solvents.
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Keiitsu SAITO, Henry FREISER
1989 年 5 巻 5 号 p.
583-586
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
The extraction of platinum(II) and/or palladium(II) with bis(2, 4, 4-trimethylpenty)phosphinodithioic acid from chloride solution has been studied. Extraction from heptane is more rapid than that from chloroform. The effect of tetraheptylammonium chloride, tetraheptylammonium thiocyanate and thiourea on the extraction of platinum(II) is examined. The rate of extraction of platinum(II) is considerably enhanced by the addition of thiourea. A rapid method for the separation of palladium(II) and platinum(II) based on the kinetic effect is proposed. Palladium(II) is first selectively extracted quantitatively into chloroform, and then platinum(II) is quantitatively (97±3%) extracted into heptane from 0.1M chloride solution containing thiourea.
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Tarek M. SAMI, Hisanori IMURA, Nobuo SUZUKI
1989 年 5 巻 5 号 p.
587-590
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
The extraction of five lanthanoid(III) ions with 2, 2′-bipyridine (bpy) from aqueous trichloroacetate (TCA) solutions to various organic solvents has been studied. The effect of pH, bpy, TCA and lanthanoid concentration on the distribution ratio of lanthanoid(III) was thoroughly investigated. The predominant species extracted into 1, 2-dichloroethane at the low-concentration region of lanthanoid(III) was found to be Ln(bpy)
2(TCA)
3. The logarithmic extraction constants for La(III), Tb(III) and Lu(III) were found to be 0.67, 1.96 and 2.41, respectively.
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María Jesús ZAPATERO, José María CASTRESAN ...
1989 年 5 巻 5 号 p.
591-596
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
The pure active constituent of LIX 54, manufactured by Henkel Co. as an extractant for copper from ammoniacal aqueous solutions, has been isolated. Structural investigations using elemental analysis and spectroscopic techniques (
1H-NMR, IR, MS) support the belief that this reagent is 1-phenyl-1, 3-decanedione. The keto-enol tautomerism of the reagent in different solvents has been discussed and quantified by means of UV-V and
1H-NMR spectroscopy.
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Mitsuhiko TAGA, Shunitz TANAKA, Masami FUKUSHIMA
1989 年 5 巻 5 号 p.
597-600
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A method for the measurement of copper complexing ability of humic acids by using diethylaminoethyl Sephadex A-25 is reported. Since the A-25 resin can adsorb copper-humic acid complexes, but not free copper ions, the free copper ions were fractionated and then determined by graphite-furnace atomic absorption spectrometry. Conditional stability constants and complexing capacities of the humic acids were estimated by Scatchard plot adapting to two-site model. The method could be applied to the humic acids from peat soil, and the copper complexing ability of the humic acids could be estimated sensitively and simply.
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Dong HUIRU, Yang SHOUZHEN, Liu JIALAI
1989 年 5 巻 5 号 p.
601-604
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
A solvent sublation-spectrophotometrc method for the determination of nickel(II) is proposed. Solvent sublation is used here to concentrate trace nickel(II) using 1-(2-pyridylazo)-2-naphthol (PAN) as a collector. At pH 3 to 11 a red complex of nickel(II) formed with PAN can be floated with the aid of small nitrogen bubbles, then dissolved in benzene. The main factors affecting the solvent sublation of nickel(II) were determined by an orthogonal experimental method. This method permits the determination of nickel(II) in the range 0-1μg/ml and can be applied to samples of drinking water with good results.
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Masatomi SAKAMOTO, Kozue OHNISHI, Yoshimi KAWAMOTO, Tomitaro ISHIMORI
1989 年 5 巻 5 号 p.
605-608
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
Two methods are proposed for the quantitative determination of zirconium and hafnium. One is based on weighing their precipitates as the tetramandelates, followed by analyses of the plateau regions observed in their thermogravimetric curves (method A); another is followed by a gravimetric determination of the ignition residue of tetramandelate in a platinum crucible (method B).
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Masatoshi MORITA, Hiroyasu ITO, Takashi UEHIRO, Kiichiro OTSUKA
1989 年 5 巻 5 号 p.
609-610
発行日: 1989年
公開日: 2007/07/27
ジャーナル
フリー
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Yuta YASAKA, Minoru TANAKA, Takashi MATSUMOTO, Koichi FUNAZO, Toshiyuk ...
1989 年 5 巻 5 号 p.
611-612
発行日: 1989年
公開日: 2007/07/27
ジャーナル
フリー
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Tsutomu NAGAOKA, Yoshihisa UCHIDA, Kotaro OGURA
1989 年 5 巻 5 号 p.
613-614
発行日: 1989年
公開日: 2007/07/27
ジャーナル
フリー
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Iwao IDA, Hiroyasu YOSHIKAWA, Youichi ISHIBASHI, Naoki GUNJI
1989 年 5 巻 5 号 p.
615-618
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
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Hiroaki NAKAYAMA, Satoshi TACHIYASHIKI, Minoru ISHII
1989 年 5 巻 5 号 p.
619-621
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
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Isao SAKANO, Sumiko KOKUBO
1989 年 5 巻 5 号 p.
623-624
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
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Yasuko T. OSANO, Takao MATSUZAKI, Ukon NAGAI
1989 年 5 巻 5 号 p.
625-626
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
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Motoo SHIRO, Koichi MORITA, Yoshio HAYASE
1989 年 5 巻 5 号 p.
627-628
発行日: 1989/10/10
公開日: 2006/06/30
ジャーナル
フリー
