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Volume 27 (1974)
- Issue 6 Pages 707-
- Issue 5 Pages 601-
- Issue 4 Pages 429-
- Issue 3 Pages 271-
- Issue 2 Pages 69-
- Issue 1 Pages 1-
Predecessor
Volume 27, Issue 6
Displaying 1-9 of 9 articles from this issue
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I. STABILITY OF SOLUTION OF NEOCARZINOSTATINMICHIHARU KOHNO, ISOKO HANEDA, YASUO KOYAMA, MIKIO KIKUCHI1974 Volume 27 Issue 6 Pages 707-714
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESSThe antitumor protein, neocarzinostatin (NCS) is an acidic single-chain molecule, crosslinked by two disulfide bridges, and consists of 109 amino acid residues. In the present study, effects on the aqueous solutioti of NCS by heat, ultraviolet and sunlight, and in the various pH solution were investigated. The present resulti have disclosed the following points:
(1) The kinetic studies of the inactivation in the aqueous solution of NCS indicated that thermal inactivation of NCS was based on the apparent first-order reaction. It was confirmed that ARRHENIUSe xpression was applicable between the rate constant and the temperature. The validity of activity in aqueous NCS solution at pH 5.0 that was reduced from initial concentration (100%) to 90% residual concentration was 13.6 months and 32 months on 10°C and 4°C, respectively.
(2) It was clarified by log κ-pH profile that aqueous NCS solution was most stable at pH 5.0.
(3) The stabilities of NCS were measured in various buffer solution, buffer concentration, isotonic saline and isotonic glucose solution. No significant change in residual activity was observed.
(4) Inactivation rate of NCS solution under sunlight was more faster than thermal one. The mode of inactivation of aqueous NCS solution did not present neither apparent first-order reaction such as heat nor zero-order reaction such as ultraviolet.(5) On the other hand, the mode of inactivation under ultraviolet showed a type of zeroorder reaction, and the inactivation rate was fast as well as the inactivation rate by sunlight.View full abstractDownload PDF (1189K) -
II. STABILITY OF INJECTION OF NEOCARZINOSTATINMICHIHARU KOHNO, FUMIO ISHII, ISOKO HANEDA, YASUO KOYAMA, MIKIO KIKUCH ...1974 Volume 27 Issue 6 Pages 715-724
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESSIn the present study, we have studied the stability of NCS injection for more than two years, the relationship between the antitumor activity against ascites tumor in rat and the antibacterial activity against Sarcina lutea, the drawing of log.κ-pH profile at 37-38°C, and the stability of NCS with the other injections and drugs. From the results obtained, the storage conditions and the estimation of the term of validity of NCS injection were discussed.
(1) No loss antibacterial activity of NCS injection was observed for two years at below 8°C.
(2) The antitumor activity of NCS injection against ascites, tumor in rat almost corresponded to the antibacterial activity against Sarcina lutea.
(3) Rate constant, on pH 5.0 calculated by log κ pH profile at 37-38°C showed a 20-fold stability compared to the rate constant at 55°C.
(4) NCS injection was inactivated by reducing reagents such as sodium bisulfide and ascorbic acid, and denaturing reagents such as urea and thiourea, but NCS injection was stable to the other various injections, drugs and reagents.View full abstractDownload PDF (1277K) -
TATSUNORI SHIMIZU1974 Volume 27 Issue 6 Pages 725-729
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESSBasic and clinical studies on the combination of antibiotics and anti-inflammatory agents have been reported by many research laboratories1). The author investigated the effect of tiaramide, a new basic anti-inflammatory agent, on serum and exudate levels of cefazolin in animals receiving a combination of tiaramide and cefazolin. The results are reported here.View full abstractDownload PDF (517K) -
Clinical experience with minocycline granulesMITSUO HIRAUMI, JUN TANI, YOSHIRO OKAMOTO, KAZURO TADA1974 Volume 27 Issue 6 Pages 730-734
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
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OTOTOXIC EFFECT OF 3', 4'-DIDEOXYKANAMYCIN B ON THE INNER EAR IN THE INTRAUTERINE GUINEA PIG EMBRYOSMASATOYO AKIYOSHI, KIICHI SATO, TADASHI SHOJI, KEI SUGAHIRO, TAYOKO TA ...1974 Volume 27 Issue 6 Pages 735-745
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESSThe present studies were performed to evaluate the transplacental ototoxic effect of 3', 4-dideoxykanamycin B (DKB), kanamycin (KM) and gentamicin (GM) on the inner ears in the intrauterine guinea pig embryos.
Intramuscular administration of the individual antibiotics to the pregnant guinea pigs of the Hartley strain started on the first day of the pregnancy. DKB was given at the doses of 100 mg/kg for 58-60 days or 75 mg/kg for 58-59 days, KM at the doses of 300 mg/kg for 58-60 days or 200 mg/kg for 53-61 days and GM at the doses of 100 mg/kg for 28 days or 40 mg/kg for 57-59 days, respectively.
The concentration of DKB and KM in the placenta, amniotic fluid and the liver and kidney of the intrauterine guinea pig embryos was measured by bioassay and it was revealed that these antibiotics can pass from the maternal guinea pigs to the intrauterine embryos.
Histopathologic examination of the new-born guinea pigs at the one month after birth disclosed that the most frequent damage of the inner ear in the new born guinea pigs was the loss of the outer hair cells confined to the organ of CORTI at the basal end of the first turn of the cochlea (40%). Relatively localized hypoplasia of the spiral ganglion in the upper part of the 4. turn associated with the mild displasia of the regional organ of CORTI occurred in 11% in the new born guinea pigs delivered from the maternal ones received DKB 75 mg/kg. The disappearance of the organ of CORTI relatively localized at the 3. turn of the cochlea was found in one guinea pig delivered from the maternal one received DKB 100 mg/kg. There was no remarkable pathologic change in the vestibular organs in the new-born guinea pigs.View full abstractDownload PDF (8219K) -
I. Acute and subacute toxicityMASAAKI KURAMOTO, YASUKO ISHIMURA, JUNKO MORIMOTO, TAKAAKI OKUBO, SHIU ...1974 Volume 27 Issue 6 Pages 746-765
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESSDownload PDF (6820K) -
IN VITRO ANTIBACTERIAL ACTIVITIES OF LVDM AGAINST CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSISTATSUNORI SHIMIZU1974 Volume 27 Issue 6 Pages 766-774
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESSLividomycin (hereinafter abbreviated as LVDM) is a new aminoglycoside antibiotic which was produced by Streptomyces lividus isolated from soil in Nagoya by Tokyo Research Laboratories of Kowa Co., Ltd.1.2) The chemical structure has been determined by ODA et al.3, 4) It is reported that LVDM has a wide range of antibacterial spectra against Gram-positive and negative bacteria, and also shows antituberculous activity8).View full abstractDownload PDF (1108K) -
HYAKUNEN TODA1974 Volume 27 Issue 6 Pages 775-778
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESSMinimum inhibitory concentration of lividomycin (LVDM) against gonococci and clinical effect of LVDM on gonorrhea were studied. The results are summarized as follows:
1) The MIC values of twelve strains of gonococci isolated from the patients treated with LVDM were about 8μg/ml, in other words, gonococci showed almost same or slightly better sensitivity to LVDM as compared with KM.
2) LVDM was intramuscularly administered once a day for 1-14 days to 15 patients with gonorrhea. Clinical effect was judged by the disappearance of gonococci which was observed under microscope and by cultivation.
3) Judging from the results obtained here, it is reasonable to state that LVDM is effective and useful in the treatment of gonorrhea when it is administered at a dosage of 2 g a day.
4) No side effect was observed.View full abstractDownload PDF (635K) -
RYUJI MATSUURA, KUNIYUKI YAMAGUCHI, MASATAKA MASUDA, KENICHI USHIJIMA, ...1974 Volume 27 Issue 6 Pages 779-786
Published: December 25, 1974
Released on J-STAGE: May 17, 2013
JOURNAL FREE ACCESS
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