In industrial fermentations of
Streptomyces aureofaciens, accumulation of hypoxanthine, cytosine and guanosine in the fermentation medium depending on the level of chlortetracycline production was observed. A similar dependance was found also in fermentation media of low- and high-producing strains of
Streptomyces aureofaciens under laboratory conditions. Chlortetracycline supplemented to the medium for the cultivation of a low- and high-productive strains of
S. aureofaciens in an amount of 200 and 500 μg/ml resulted in an increase of the hypoxanthine and cytosine levels. At the same time AMP and CMP in some cases were also found in the medium. Cadaverine added to the fermentation medium in an amount of 0.1 % decreased the level of cytosine by 60% and increased the guanosine content by 50% as compared with the control. The hypoxanthine level remained unchanged. In the acid-soluble fraction of the mycelium of
S. aureofaciens nucleotides, nucleosides, and bases with predominance of AMP and its degradation products were found. In the fermentation medium and in the acid-soluble fraction of the mycelium, some unknown ultraviolet-absorbing substances were observed.
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