The Journal of Antibiotics

ANTIBIOTIC YC 73 OF PSEUDOMONAS ORIGIN. I

PRODUCTION, ISOLATION AND PROPERTIES

YOSHIYUKI EGAWA, KIMIO UMINO, SHIGEMI AWATAGUCHI, YOSHISHIGE KAWANO, T ...

1970 Volume 23 Issue 6 Pages 267-270
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.267

JOURNAL FREE ACCESS

Show abstractHide abstract

A dark green antibiotic designated as YC 73 was isolated from the culture broth of a pseudomonad. The antibiotic (C₄H₈N₂O₂S₂Cu) proved to be hitherto unknown and is characterized by the presence of sulfur and copper atoms in its molecule. It is active against bacteria and fungi.

View full abstract

Download PDF (241K)
STUDIES ON THE MODE OF ACTION OF ALTHIOMYCIN

HIDEO FUJIMOTO, TADATOSHI KINOSHITA, HIDEO SUZUKI, HAMAO UMEZAWA

1970 Volume 23 Issue 6 Pages 271-275
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.271

JOURNAL FREE ACCESS

Show abstractHide abstract

Among syntheses of DNA, RNA and protein in intact cells of E. coli B, althiomycin exhibited the strongest inhibition against protein synthesis. In the cell-free system althiomycin was confirmed to inhibit protein synthesis. On the other hand, it did not inhibit protein synthesis of reticulocyte either in the intact cells or in the cell-free system. By further studies in E. coli B systems the antibiotic was shown to inhibit the puromycin reaction, without inhibiting aminoacyl-tRNA synthesis and binding of aminoacyl-tRNA to ribosomes.

View full abstract

Download PDF (288K)
COMBINATIONS OF AGENTS WHICH INDUCE ATP ENERGIZED MITOCHONDRIAL VOLUME CHANGES

HERBERT I. HADLER, BOB E. CLAYBOURN, TAI PO TSCHANG

1970 Volume 23 Issue 6 Pages 276-287
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.276

JOURNAL FREE ACCESS

Show abstractHide abstract

The ATP energized mitochondrial volume change phenomena employing the antibiotic and antitumor agent showdomycin has visualized the interaction with mitochondria of a respiratory inhibitor antimycin and an uncoupling agent 2, 4-dinitrophenol. These studies have been extended to other well known agents which interact with mitochondria. Dicoumarol, carbonyl cyanide p-trinuoromethoxyphenylhydrazone and heat treatment mimicked 2, 4-dinitrophenol. N-Ethylmaleimide resembled showdomycin. Valinomycin behaved like gramicidin. Our previous concept of a strategically located pivotal mitochondrial thiol group rationalized the behavior of all the above agents and hence this concept is of general significance.

View full abstract

Download PDF (522K)
BINDING OF BLASTICIDIN S TO RIBOSOMES

TADATOSHI KINOSHITA, NOBUO TANAKA, HAMAO UMEZAWA

1970 Volume 23 Issue 6 Pages 288-290
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.288

JOURNAL FREE ACCESS

Show abstractHide abstract

The binding of blasticidin S to ribosomes is demonstrated using equilibrium dialysis. There is a single binding site per each ribosome and the association constant is 5×10⁵ M^-1. The binding site is localized in the 50S ribosomal subunit. The binding of blasticidin S to ribosomes is inhibited by gougertotin, but not affected by chloramphenicol, lincomycin, erythromycin or puromycin.

View full abstract

Download PDF (201K)
ISOLATION OF A NEW WATER-SOLUBLE BASIC ANTIBIOTIC A-396-I

JUN'ICHI SHOJI, SHUICHI KOZUKI, MIKAO MAYAMA, YOSHIMI KAWAMURA, KOICHI ...

1970 Volume 23 Issue 6 Pages 291-294
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.291

JOURNAL FREE ACCESS

Show abstractHide abstract

Two water-soluble basic antibiotics A-396-I and II were isolated from a culture broth of Streptoverticillium eurocidicus A-396. Both antibiotics gave similar analytical data indicative of a molecular formula C_19±1H_35±20₁₃N₃, but different optical activities : A-396-I, [α]_D +11.6° (in water), and A-396-II, [α]_D +21.8° (in water). A comparison with known antibiotics using thin-layer chromatography suggested that A-396-I is a new antibiotic and A-396-II is identical with hygromycin B.

View full abstract

Download PDF (222K)
A NEW ANTIBIOTIC, BULGERIN, ACTIVE AGAINST PHYTOPATHOGENIC FUNGI

JUN'ICHI SHOJI, RYUZI SAKAZAKI, MIKAO MAYAMA, YOSHIMI KAWAMURA, YUKIO ...

1970 Volume 23 Issue 6 Pages 295-299
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.295

JOURNAL FREE ACCESS

Show abstractHide abstract

A new antibiotic, bulgerin, was isolated from a culture broth of a streptomyces strain S-288. The strain was classified as Streptomyces aburaviensis var. tuftformis. The antibiotic is a water-soluble amphoteric substance, C₁₇H₂₄O₁₀N₄, λ_max 230 mμ (shoulder) and 289 mμ in 0.05 N HCl, [α]_D +24.0° in water, and is active against some phytopathogenic fungi.

View full abstract

Download PDF (337K)
LETHAL EFFECT OF BLEOMYCIN ON CULTURED MAMMALIAN CELLS

TOYOZO TERASIMA, HAMAO UMEZAWA

1970 Volume 23 Issue 6 Pages 300-304
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.300

JOURNAL FREE ACCESS

Show abstractHide abstract

By using cultured mammalian cells lethal effect of bleomycin was measured in terms of mean lethal dose (D₃₇) ; the cytotoxicity on proliferative system was moderate as compared with those of other anti-tumor agents ; the dose-response revealed marked difference between two mammalian cells used. The pattern of drug response during the cell cycle was demonstrated by virtue of the synchrony method. The similarity to the pattern of X-ray response strongly suggested the involvement of the same mechanism in lethal action of bleomycin.

View full abstract

Download PDF (277K)
THE HUMAN TUMOR-EGG HOST SYSTEM

IV. DISCOVERY OF A NEW ANTI-TUMOR AGENT, COMPOUND 593 A

C. O. GLTTERMAN, E. L. RLCKES, D. E. WOLF, J. MADAS, S. B. ZIMMERMAN, ...

1970 Volume 23 Issue 6 Pages 305-310
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.305

JOURNAL FREE ACCESS

Show abstractHide abstract

A new crystalline anti-tumor agent, compound 593A, has been discovered in fermentation broths of Streptomyces griseoluteus. Its presence in the fermented broth was detected by the human tumor-egg host system which was used also to help guide fermentation and isolation studies. On a weight basis, against the human adenocarcinoma (H. Ad.) # 1 in the embryonated egg, antitumor compound 593A is about 100 times as potent as hadacidin, about 4 times as potent as sodium L-tenuazonate, and about one-half as potent as mitomycin C . It also inhibits, in the egg, growth of the humansarcoma(H. S.)#1, and metastasis but not primary growth of the human epidermoid carcinoma (H. Ep.) #3. Anti-tumor compound 593A has the empirical formula C₇H₁₁ON₂Cl.

View full abstract

Download PDF (334K)
ON THE SITE OF ACTION OF BOTTROMYGIN A₂

TADATOSHI KINOSHITA, NOBUO TANAKA

1970 Volume 23 Issue 6 Pages 311-312
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.311

JOURNAL FREE ACCESS

Download PDF (106K)
EQJJIMOLECULAR REACTION OF SHOWDOMYCN WITH THIOLS

SACHIHIKO WATANABE

1970 Volume 23 Issue 6 Pages 313-314
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.313

JOURNAL FREE ACCESS

Download PDF (85K)
A NEW ANTIBIOTIC, KINAMYCIN

SHINYA ITO, TAMIKO MATSUYA, SATOSHI OMURA, MASARU OTANI, AKIRA NAKAGAW ...

1970 Volume 23 Issue 6 Pages 315-317
Published: June 25, 1970
Released on J-STAGE: April 12, 2006

DOIhttps://doi.org/10.7164/antibiotics.23.315

JOURNAL FREE ACCESS

Download PDF (159K)

Register with J-STAGE for free!