The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Volume 24 , Issue 2
Showing 1-12 articles out of 12 articles from the selected issue
  • TATIANA F. KUIMOVA, KAZUTAKA FUKUSHIMA, SHYUKO KURODA, TADASHI ARAI
    1971 Volume 24 Issue 2 Pages 69-76
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    A further characterization of Actinomyces tumemacerans KRASSILNIKOV and KOVESHNIKOV, 1962 strain INMI P-42 was carried out. It is classified in Section Monoverticillus-Spira, Series white to gray. When the organism was shakecultured in a wheat flour medium, an antibacterial antibiotic with limited antitumor activity was isolated from the culture filtrate and a tetraene antibiotic from the mycelial cake. The antibacterial antibiotic proved to be closely related to or identical with BA-180265 A (kanchanomycin) reported by Liu et al. in 1963.
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  • AKIHIRO MATSUMAE, E. G. CANTINO
    1971 Volume 24 Issue 2 Pages 77-84
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The MIC of 48 antibiotics and other antifungal agents was established for the wild type and six mutants of Blastocladiella emersonii, as well as B. britannica, B. simplex, B. cystogena, and the related Allomyces macrogynus. Meiospores and mitospores of A. macrogynus differed from RS and OG spores of B. entersonii primarily in their responses to tetracycline, trichomycin, mitomycin C, antimycin A, and actinomycin, as well as bis(dimethylthiocarbamoyl) disulfate (T-l) and acrinavin (T-13). The four wild type species of Blastocladiella differed from one another primarily in their responses to mitomycin C, actinomycin S, chloramphenicol, endomycin, chlortetracycline, and trichomycin, as well as pentachloronitrobenzene (T-7), 2, 4-dichloro-6-(2-chloranylyno)-S-triazine (T-8), 2, 2/-methylene bis(3, 4, 6 trichlorophenol) (T-9), 3, 4, 4'-trichlorocarbanilide (T-4), malachite green (T-12), and T-13. The wildtype B. emersonii differed from its albino mutants primarily in its response to antimycin A, T-4, and (T-8). The Blastocladiaceae were also compared with two yeasts and Staphylococcus aureus in their response to various toxicants.
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  • ISOLATION AND THE STRUCTURE OF A HYDROLYSIS PRODUCT
    SUMIO UMEZAWA, TAKAYUKI USUI, HAMAO UMEZAWA, TSUTOMU TSUCHIYA, TOMIO T ...
    1971 Volume 24 Issue 2 Pages 85-92
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    A new streptomycete metabolite sphydrofuran was isolated from the culture filtrate of the strain MC41-M1 and MC340-A1 by a chemical screening method using Ehrlich reagent. Sphydrofuran was an acid-unstable substance and the structure of an acid-hydrolysis product was determined as 2-methyl- .4-(l-glyceryl) furan.
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  • THE STRUCTURE OF SPHYDROFURAN
    TAKAYUKI USUI, SUMIO UMEZAWA, TSUTOMU TSUCHIYA, HIROSHI NAGANAWA, TOMI ...
    1971 Volume 24 Issue 2 Pages 93-106
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The structure of a new streptomycete metabolite sphydrofuran was determined as an anomeric mixture of 3, 4, 5'-trmydroxy-S'-methyl-2, 3-spirobi(tetrahydrofuran) (A1 and A2) which is brought into an equilibrium mixturewith trans-3, cis-4-dihydroxy-trans-2-hydroxymethyl-2-acetonyl (tetrahydrofuran)(A3) in some solvents.
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  • PRODUCTION AND BIOLOGICAL PROPERTIES OF VALIDAMYCINS A AND B
    TAKASHI IWASA, EIJI HIGASHIDE, HIROICHI YAMAMOTO, MOTOO SHIBATA
    1971 Volume 24 Issue 2 Pages 107-113
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The fermentation conditions for the production of validamycin by Streptomyces hygroscopicus var. limoneus No. T-7545 were studied. Validamycins A and B showed no antimicrobial activity on agar media against bacteria, yeasts and fungi, but caused an abnormal branching at the tips of the hyphae of Pellicularia sasakii. Although they were inactive in vitro, validamycins A and B controlled the sheath blight of rice plants and the damping off of cucumber seedlings in green house tests. Validamycin A was effective against the sheath blight at a concentration of 30ppm. Both antibiotics showed low toxicity to plants, fishes and mice.
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  • BIOASSAY METHODS FOR THE DETERMINATION OF VALIDAMYCIN
    TAKASHI IWASA, EIJI HIGASHIDE, MOTOO SHIBATA
    1971 Volume 24 Issue 2 Pages 114-118
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Validamycin was found to cause an abnormal branching at the tips of the hyphae of Pellicularia sasakii under specific conditions. By application of this phenomenon, an agar dilution method, "dendroid-test method", was established. In another method called the "reversed layer method", agar plates inoculated with P. sasakii are incubated for 40 to 45 hours, then covered with more agar medium and paper disks dipped in the sample solution are applied on the surface. After overnight incubation the diameters of the inhibition zones are measured.
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  • ISOLATION AND CHARACTERIZATIN OF VALIDAMYCINS A AND B
    TAKASHI IWASA, YUKIHIKO KAMEDA, MITSUKO ASAI, SATOSHI HORII, KOMEI MIZ ...
    1971 Volume 24 Issue 2 Pages 119-123
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    Newantibiotics named validamycins A and B, active against the sheath blight of rice plants, were isolated from the culture nitrate of Streptomyces hygroscopicus var. limoneus. Validamycins A and B are water-soluble, glucosecontaining basic antibiotics, and with molecular formulae of C20H3337NO1314 and C20H3337NO1415, respectively.
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  • STRUCTURE AND SYNTHESIS OF THIOFORMIN AND ITS CUPRIC COMPLEX (YC 73)
    YOSHIYUKI EGAWA, KIMIO UMINO, YUKIO ITO, TOMOHARU OKUDA
    1971 Volume 24 Issue 2 Pages 124-130
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    The structure of thioformin, a new acidic antibiotic substance obtained by removing cupric ion from antibiotic YC 73, was shown to be N-methyl-N-thioformylhydroxylamine by degradative and synthetic studies. Numerous metal complexes of thioformin, including the cupric (antibiotic YC 73 itself) and ferric complexes, were also synthesized and characterized.
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  • SYNTHESIS OF THIOFORMIN ANALOGUES
    YUKIO ITO, KIMIO UMINO, TOSHIO SEKIGUCHI, TOSHIKAZU MIYAGISHIMA, YOSHI ...
    1971 Volume 24 Issue 2 Pages 131-134
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
    N-Substituted thioformylhydroxylamine derivatives represented by the general formula R HO-N-C-S H were synthesized and examined for antimicrobial activity. These compounds were analogues of an antibiotic thioformin which was confirmed to be N-methyl-N-thioformylhydroxylamine.
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  • K.L. PERLMAN, J. WALKER, D. PERLMAN
    1971 Volume 24 Issue 2 Pages 135-136
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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  • J. L. WITCHITZ, Y. A. CHABBERT
    1971 Volume 24 Issue 2 Pages 137-139
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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  • KUNIKATSU SHIRAHATA, TADATOSH HAYASHI, ISAO MATSUBARA
    1971 Volume 24 Issue 2 Pages 140-141
    Published: February 25, 1971
    Released: April 12, 2006
    JOURNALS FREE ACCESS
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