-
HIROSHI MAEDA
1974 Volume 27 Issue 5 Pages
303-311
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
The proteinous antibiotic neocarzinostatin was succinylated using succinic anhydride, and two
bis-succinyl compounds, neocarzinostatin derivatives (SUC-I and SUC-II), were obtained. The biological and chemical properties of SUC-I and SUC-II were investigated after separation by column chromatography. The only free amino groups in neocarzi-nostatin were succinylated completely in both derivatives. Amino terminal analysis, electrophoretic mobility and amino acid analysis were the same for both derivatives. However, only SUC-II was biologically active when assayed on three human cell lines and
Sarcina lutea. Analysis by UV spectroscopy and circular dichroism indicated a difference in the molecular conformation, perhaps a slightly losened structure in SUC-I as compared with SUC-II. These isologous derivatives with different biological and optical activity have also been observed in neocarzinostatin. SUC-I seems to be a counterpart of "pre-NCS", an inactive form of NCS. SUC-II differs from NCS in being completely devoid of cytolytic and cytocidal activity. The retention of biological activity in
bis-N-succinyl neocarzinostatin, in which all amino groups are bolocked, indicates that in NCS free amino groups are not essential for activity.
View full abstract
-
MIKLOS BODANSZKY, AGNES BODANSZKY
1974 Volume 27 Issue 5 Pages
312-315
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
Base-catalyzed addition of thioglycolic acid to the dehydrobutyrine residue in stendomycinfollowed by hydrolysis and isolation of the newly formed amino acid (S-carboxy-methyl-β-methylcysteine) provided a pair of optically active diastereoisomers. Thus, the addition of the thiol to the double bond is stereoselective and is governed by thearchitecture of the molecule of the antibiotic.
View full abstract
-
IBRAHIM R. SHIMI
1974 Volume 27 Issue 5 Pages
316-320
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
Kuwaitimycin influences the translocation of alkali metal cations in liver mitochondria.In presence of valinomycin, which stimulates uptake of potassium ions by the mitochondria, the addition of kuwaitimycin could augment the loss of these ions when the source of energy was mainly an oxidizable substrate. However, kuwaitimycin fail ed to cause loss of K
+ from mitochondria in presence of appropriate amounts of ATP. A relation between the ionic size of the alkali metal cations and the inhibiting effect of the antibiotic on glutamate oxidation could be demonstrated. The ability of kuwaitimycin to restrain ATP supported oscillatory swelling associated with alkali metal cations transport induced by monazomycin was also demonstrated. The more voluminous the cation the more pronounced the effect on cation transport. The effect of kuwaitimycin on ATP hydrolysis in presence of different alkali metal cations
was found to be weak and independent or the cationic size.In light of the results obtained it is suggested that the antibiotic is exerting its effect on ion transport by inducing pores in the mitochondrial membrane and that the addition of appropriate amounts of ATP might alter the conformation of the mitochondrial membrane in such a way as to nullify the effect of the antibiotic.
View full abstract
-
A. D. ARGOUDELIS, A. DIETZ, L. E. JOHNSON
1974 Volume 27 Issue 5 Pages
321-328
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
Zervamicins I and II are new polypeptide antibiotics produced by
Emericellopsis salmosynnemata. Zervamicin I is an acidic compound while zervamicin II is a neutral polypeptide like emerimicins, antiamoebin and stilbellin to which both zervamicins are related.
View full abstract
-
I. PRODUCING ORGANISM, FERMENTATION AND ISOLATION
MAMORU ARAI, TATSUO HANEISHI, RYUZO ENOKITA, HISASHI KAYAMORI
1974 Volume 27 Issue 5 Pages
329-333
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
Aspiculamycin, a new cytosine nucleoside with versatile antibiotic activity, wasproduced by a strain of
Streptomyces designated as
S. toyocaensis var.
aspiculamyceticus.The antibiotic was produced in submerged fermentation together with two otherantifungal antibiotics, toyocamycin and an unidentified tetraene antibiotic. Isolation ofaspiculamycin was proceeded by ion-exchange column chromatography of the culturefiltrate on IRC-50 followed by rechromatography on CG-50.
View full abstract
-
II. PHYSICO-CHEMICAL PROPERTIES AND STRUCTURAL ELUCIDATION
TATSUO HANEISHI, AKIRA TERAHARA, MAMORU ARAI
1974 Volume 27 Issue 5 Pages
334-338
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
Aspiculamycin (
I), C
19H
30N
8O
10, is a water-soluble basic antibiotic with versatile biological activities. From its physico-chemical properties, it has been characterized as one of the cytosine-nucleoside antibiotics for which the structure, 1-(4-sarcosyl-D-seryl-D-serylamino-4-deoxy-β-D-glucopyranosyluronamide) cytosine, is proposed.
View full abstract
-
TATSUO HANEISHI, MAMORU ARAI, NORITOSHI KITANO, SHINJIRO YAMAMOTO
1974 Volume 27 Issue 5 Pages
339-342
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
KOICHI OGATA, KOZO UEDA, TORU NAGASAWA, YOSHIKI TANI
1974 Volume 27 Issue 5 Pages
343-345
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
FUJIO SUZUKI, TOSHIO SUGANUMA, NAKAO ISHIDA
1974 Volume 27 Issue 5 Pages
346-348
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
KOJI O'HARA, MEGUMI KONO, SUSUMU MITSUHASH
1974 Volume 27 Issue 5 Pages
349-351
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
THE X-RAY STRUCTURE DETERMINATION OF 4-AMINO-3-HYDROXY-2-METHYL-n-VALERIC ACID, AN AMINE COMPONENT OF BLEOMYCIN
HIKARU NAKAMUA, TOMOHISA TAKITA, HAMAO UMEZAWA, YASUHIKO MURAOKA, YOIC ...
1974 Volume 27 Issue 5 Pages
352-355
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
BIOGENETIC-LIKE SYNTHESIS OF (2S, 3S, 4R)-4-AMINO-3-HYDROXY-2-METHYL-n-VALERIC ACID, AN AMINE COMPONENT OF BLEOMYCIN
TAKEO YOSHIOKA, TAKESHI HARA, TOMOHISA TAKITA, HAMAO UMEZAWA
1974 Volume 27 Issue 5 Pages
356-357
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
HAMAO UMEZAWA, YUJI MATSUHASHI, MORIMASA YAGISAWA, HARUO YAMAMOTO, SHI ...
1974 Volume 27 Issue 5 Pages
358-360
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
TOSHIHIKO KANZAKI, TADAZUMI FUKITA, HIDEO SHIRAFUJI, YUKIO FUJISAWA, K ...
1974 Volume 27 Issue 5 Pages
361-362
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
SATOSHI OMURA, HARUO TANAKA, YASUAKI KOYAMA, RUIKO OIWA, MICHIKO KATAG ...
1974 Volume 27 Issue 5 Pages
363-365
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
TAKASHI OSONO, KIRUKO MORIYAMA, MASUO MURAKAMI
1974 Volume 27 Issue 5 Pages
366-369
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS
-
SATOSHI OMURA, AKIRA NAKAGAWA, KAZUHIRO SUZUKI, TOJU HATA
1974 Volume 27 Issue 5 Pages
370-372
Published: 1974
Released on J-STAGE: April 12, 2006
JOURNAL
FREE ACCESS