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I. PRODUCTION, ISOLATION, AND CHARACTERIZATION
Y. K. TONY LAM, DAVID L. WILLIAMS, JANET M. SIGMUND, MANUEL SANCHEZ, O ...
1992 Volume 45 Issue 11 Pages
1709-1716
Published: November 25, 1992
Released on J-STAGE: April 19, 2006
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Cochinmicins I, II, III are novel peptolides produced in submerged-fermentation cultures of
Microbispora sp. ATCC 55140. These closely related compounds are separated by HPLC and are novel competitive endothelin antagonists. Cochinmicins II and III are stereoisomeric to each other. Cochinmicin I is the deschloro analog of cochinmicin III.
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II. STRUCTURE DETERMINATION
DEBORAH ZINK, OTTO D. HENSENS, Y. K. TONY LAM, ROBERT REAMER, JERROLD ...
1992 Volume 45 Issue 11 Pages
1717-1722
Published: November 25, 1992
Released on J-STAGE: April 19, 2006
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Cochinmicins I, II, and III are competitive endothelin antagonists produced by
Microbispora sp. ATCC 55140. The cochinmicins are cyclic depsipeptides containing six α-amino acids and a pyrrolecarboxylic acid. Based upon MS, ID and 2D NMR, and LC data, the structures and absolute stereochemistries of the cochinmicins have been assigned. All three components have the same basic sequence and contain one equivalent each of D-
allo-threonine, D-alanine, L-phenylalanine, D-phenylalanine, 5-chloropyrrole-2-carboxylic acid (or pyrrole-2-carboxylic acid in cochinmicin I), plus two equivalents of 3, 5-dihydroxyphenylglycine (DHPG). The phenylalanine residues were differentiated
via a methanolysis product which contained only one of the phenylalanine residues. Both DHPG residues have the D configuration in the more active cochinmicins I and III. Cochinmicin II contains both D- and L-DHPG and these residues have been differentiated in the sequence based upon
1H NMR data.
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I. TAXONOMY, FERMENTATION, ISOLATION, AND PHYSICO-CHEMICAL PROPERTIES OF MATLYSTATIN-GROUP COMPOUNDS
TAKESHI OGITA, AKIRA SATO, RYUZO ENOKITA, KEIKO SUZUKI, MIYUKI ISHII, ...
1992 Volume 45 Issue 11 Pages
1723-1732
Published: November 25, 1992
Released on J-STAGE: April 19, 2006
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During the course of a screening for inhibitors of typelV collagenases, new metabolites, designated matlystatins, have been isolated from an actinomycete strain, which was identified as a strain of
Actinomadura atramentaria. Matlystatins were composed of five congeners, which were separated and purified by n-butanol extraction and chromatography.
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II. BIOLOGICAL ACTIVITIES
KAZUHIKO TANZAWA, MIYUKI ISHII, TAKESHI OGITA, KOHEI SHIMADA
1992 Volume 45 Issue 11 Pages
1733-1737
Published: November 25, 1992
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Matlystatin A, the main component of matlystatins, inhibits 92kDa and 72kDa typeIV collagenases with IC
50 values of 0.3μM and 0.56μM, respectively, while 7- to 11-fold greater concentrations are required to inhibit thermolysin and aminopeptidase M. The inhibition is reversible and competitive with respect to gelatin. It inhibits the invasion of basement membrane Matrigel by human fibrosarcoma HT1080 dose-dependently with an IC
50 value of 21.6μM.
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MURALEEDHARAN G. NAIR, SAROJ K. MISHRA, ALAN R. PUTNAM, RAMESH C. PAHD ...
1992 Volume 45 Issue 11 Pages
1738-1745
Published: November 25, 1992
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Spartanamicins A and B, two antifungal antibiotics, were produced by a culture of
Micromonospora spp. strain No. MSU-43097 (ATCC 53803), isolated from a potted soil containing asparagus (
Asparagus officinalis L.) plants. The antibiotics were isolated from the mycelial cake using organic solvents. The structures of Spartanamicins A and B were determined by spectral and chemical means. Spartanamicin B is more active as an antifungal compound than it's analogue, A. The minimum inhibitory concentration for Spartanamicin B on
Candida albicans and
Aspergillus,
Cladosporium,
Cryptococcus,
Rhodotorula and
Staphylococcus spp. ranged from 0.2 to 1 μg/ml. It was not active against
Staphylococcus aureus,
Escherichia coli and
Citrobacter spp. but some strains of
S. aureus were sensitive.
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MINORU HANADA, KOKO SUGAWARA, KEIKO KANETA, SOICHIRO TODA, YUJI NISHIY ...
1992 Volume 45 Issue 11 Pages
1746-1752
Published: November 25, 1992
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An actinomycete strain No. Q996-17 produced a novel compound, epoxomicin, which exhibited
in vivo antitumor activity against B16 melanoma. Structural studies indicated that it is a new member of the epoxy-β-aminoketone group, and is closely related to eponemycin.
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I. TAXONOMY, FERMENTATION, ISOLATION AND BIOLOGICAL ACTIVITY
M. L. FIDALGO, J. L. ALONSO, J. SOLIVERI, M. E. ARIAS
1992 Volume 45 Issue 11 Pages
1753-1758
Published: November 25, 1992
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APHE-1 and APHE-2 are two new antibiotics produced by
Streptoverticillium griseocarneum NCIMB 40447. They exhibited a remarkable cytotoxic activity against several tumor cell lines from different origin. Furthermore, they showed weak antimicrobial activity against Gram-positive bacteria, filamentous fungi and yeasts.
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II. PHYSICO-CHEMICAL PROPERTIES AND STRUCTURE ELUCIDATION
M. L. FIDALGO, M. S. ARIAS, J. SOLIVERI, M. E. ARIAS
1992 Volume 45 Issue 11 Pages
1759-1762
Published: November 25, 1992
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Two new pyrazolo-isoquinolinone antibiotics, APHE-1 and APHE-2, have been isolated from the culture nitrate and mycelia of
Streptoverticillium griseocarneum NCIMB 40447. Molecular formulae were established as C
13H
12N
2O for APHE-1 and C
14H
14N
2O for APHE-2, by elemental analysis, NMR and mass spectra. 2D NMR techniques (
1H-
1H COSY-45 and
1H-
13C correlated spectroscopy) have been applied to establish their structures.
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YOKO IKEDA, SHUICHI GOMI, MASA HAMADA, SHINICHI KONDO, TOMIO TAKEUCHI
1992 Volume 45 Issue 11 Pages
1763-1766
Published: November 25, 1992
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A streptomyces antibiotic, bellenamine has been produced in a simple synthetic medium consisting of D-galactose, dextrin, ammonium sulfate and calcium carbonate. Three new minor metabolites,
N-(aminomethyl)succinamic acid, 1'-
N-acetylbellenamine and D-β-lysinamide have been isolated from the synthetic medium culture. They showed no antibiotic activity.
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TAKAYUKI AOYAMA, HIROSHI NAGANAWA, YASUHIKO MURAOKA, TAKAAKI AOYAGI, T ...
1992 Volume 45 Issue 11 Pages
1767-1772
Published: November 25, 1992
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The biosynthesis of benastatin A, produced by
Streptomyces sp. MI384-DF12, has been studied by feeding experiments with
14C- and
13C-labeled compounds followed by measurement of radioactivity and
13C NMR analysis. The results indicate that benastatin A is derived from two methionine units and fourteen acetate units, condensed in the "head-to-tail" fashion of typical polyketide biosynthesis.
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M. S. KUO, D. A. YUREK, J. H. COATS, S. T. CHUNG, G. P. LI
1992 Volume 45 Issue 11 Pages
1773-1777
Published: November 25, 1992
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An accumulated lincomycin intermediate in UC 8292, a lincomycin nonproducing strain of
Streptomyces lincolnensis, has been isolated and purified by employing an assay system based on complementation of UC 11066, another lincomycin nonproducing strain of
S. lincolnensis. The structure of the purified intermediate is shown to be 3-propylidene-Δ
1-pyrroline-5-carboxylic acid, or 1, 2, 3, 6-tetradehydro-propylproline by mass spectrometry and NMR spectroscopic studies. Based on the structure of this newly found intermediate, a biosynthetic pathway for propylproline is proposed as tyrosine→L-3-hydroxytyrosine (Dopa)→→→→3-propylidene-Δ
1-pyrroline-5-carboxylic acid→3-propyl-Δ
2-pyrroline-5-carboxylic acid→propylproline.
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KAYO AOYAGI, NORIKO ITOH, FUMINORI ABE, SHIGERU ABE, KATSUHISA UCHIDA, ...
1992 Volume 45 Issue 11 Pages
1778-1784
Published: November 25, 1992
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Ubenimex is a low molecular weight microbial metabolite which has been demonstrated to have antitumor and immunomodulatory activities. In this study, the protective effect of ubenimex on
Candida albicans infection was investigated in normal and immunosuppressed mice. In normal mice, treatment with ubenimex at 0.5, 5 and 25mg/kg for 5 days prior to infection prolonged survival time in a dose-dependent manner. In immunosuppressed mice treated with a single dose of cyclophosphamide 4 days prior to infection, ubenimex treatment at 5 mg/kg for 5 days significantly increased the number of survivors. Ubenimex-treated mice had a significant increase in number of peritoneal exudate cells with neutrophils as well as enhanced functions, including phagocytosis and active oxygen production. These results suggest the potential usefulness of ubenimex as a prophylactic agent for the management of patients with opportunistic fungal infections.
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A. BRIAN JONES, CHARMAINE M. HERBERT
1992 Volume 45 Issue 11 Pages
1785-1791
Published: November 25, 1992
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The
in vitro antimicrobial potency of 10-aza-9-deoxo-11-deoxyerythromycin A, the first member of a new class of macrolide antibiotic, was determined. Several other members of this family of azalide were prepared and similarly screened in order to begin to define the antibiotic potential of the class. The results indicate that the SAR for this structural type parallels that of other macrolides and that it offers no apparent benefit over known 15-membered azalides.
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Y. K. TONY LAM, DEBORAH L. ZINK, DAVID L. WILLIAMS, BRUCE W. BURGESS
1992 Volume 45 Issue 11 Pages
1792-1794
Published: November 25, 1992
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TAKAAKI AOYAGI, MASAHIRO HATSU, CHIAKI IMADA, HIROSHI NAGANAWA, YOSHIR ...
1992 Volume 45 Issue 11 Pages
1795-1796
Published: November 25, 1992
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SEIICHI TANAKA, MITSURU OHKUBO, KATSUHISA KOJIRI, HIROYUKI SUDA, AKIHI ...
1992 Volume 45 Issue 11 Pages
1797-1798
Published: November 25, 1992
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SYNTHESIS OF THE TWO ISOMERIC DEOXYANHYDRONAPHTHOPYRANONES FROM TORALACTONE
DEMISE PARISOT, MICHEL DEVYS, MICHEL BARBIER
1992 Volume 45 Issue 11 Pages
1799-1801
Published: November 25, 1992
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DIDIER VAN DER PYL, JUNJI INOKOSHI, KAZURO SHIOMI, HONG YANG, HIDEO TA ...
1992 Volume 45 Issue 11 Pages
1802-1805
Published: November 25, 1992
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HEIKO PATZELT, VERONIQUE GAUDET, JOHN A. ROBINSON
1992 Volume 45 Issue 11 Pages
1806-1808
Published: November 25, 1992
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MONTGOMERY E. FAVRET, LA VERNE D. BOECK
1992 Volume 45 Issue 11 Pages
1809-1811
Published: November 25, 1992
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4. THE BIOSYNTHETIC ORIGIN OF THE METHYL GROUP OF FOSFOMYCIN
TOMOHISA KUZUYAMA, TOMOMI HIDAKA, KAZUMA KAMIGIRI, SATOSHI IMAI, HARUO ...
1992 Volume 45 Issue 11 Pages
1812-1814
Published: November 25, 1992
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HIROAKI TAKAYANAGI, JUN-ICHI OSHIMA, KIMIO FURUHATA, KAZUYOSHI TAKEDA, ...
1992 Volume 45 Issue 11 Pages
1815-1817
Published: November 25, 1992
Released on J-STAGE: April 19, 2006
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