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ICHIJI NAMATAME, HIROSHI TOMODA, SHUYI SI, YUICHI YAMAGUCHI, ROKURO MA ...
1999 Volume 52 Issue 1 Pages
1-6
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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Beauveria sp. FO-6979, a soil isolate, was found to produce inhibitors of lipid droplet formation in mouse peritoneal macrophages. A new compound beauveriolide III was isolated along with a known compound beauveriolide I from the fermentation broth of the producing strain by solvent extraction, ODS column chromatography, silica gel column chromatography and preparative HPLC. Beauveriolides I and III caused a reduction in the number and size of cytosolic lipid droplets in macrophages at 10 μM without any cy to toxic effect on macrophages.
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ICHIJI NAMATAME, HIROSHI TOMODA, NORIKO TABATA, SHUYI SI, SATOSHI OMUR ...
1999 Volume 52 Issue 1 Pages
7-12
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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The structure of fungal beauveriolide III, an inhibitor of lipid droplet formation in mouse macrophages, was elucidated to be cyclo-[(3
S, 4
S)-3-hydroxy-4-methyloctanoyl-Lphenylalanyl-L-alanyl-D-allo-isoleucyl] by spectral analyses and chemical degradation.
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HISAYUKI KOMAKI, AKIRA NEMOTO, YASUSHI TANAKA, HIROAKI TAKAGI, KATSUKI ...
1999 Volume 52 Issue 1 Pages
13-19
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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A novel tricyclic diterpenoid antibiotic, brasilicardin A, was isolated from the culture broth of
Nocardia brasiliensis IFM 0406. The antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (MLR) assay system and its IC
50 value was 0.057 μg/ml. Although the inhibitory activity of cyclosporin A (CyA) against IL-2 production was confirmed in the MLR assay system, brasilicardin A did not have the activity. The results of
in vitro toxicity testing of brasilicardin A against various human cell lines were compared with those of CyA.
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I. Taxonomy, Fermentation, Isolation and Biological Activity
MASATOMI IIJIMA, TETSUYA SOMENO, CHIAKI IMADA, YOSHIRO OKAMI, MASAAKI ...
1999 Volume 52 Issue 1 Pages
20-24
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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IC202A, a new immunosuppressive compound, was isolated from the culture filtrate of
Streptoalloteichus sp. 1454-19. It showed a suppressive effect on mixed lymphocyte culture reaction with an IC
50 value of 3.6 μg/ml and mitogen induced lymphocyte blastogenesis
in vitro.
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II. Physico-chemical Properties and Structure Elucidation
MASATOMI IIJIMA, TETSUYA SOMENO, MASAHIDE AMEMIYA, RYUICHI SAWA, HLROS ...
1999 Volume 52 Issue 1 Pages
25-28
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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IC202A (
1) was isolated from the culture filtrate of
Streptoalloteichus sp. 1454-19. The structure of
1 was determined by spectral analysis including a variety of twodimentional NMR and FAB-MS experiments. IC202A is a ferrioxamine-related compound containing a butylidene
N-oxide function.
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I. Production, Isolation and Biological Properties
HUA ZHANG, HIROSHI TOMODA, NORIKO TABATA, MASAKO OOHORI, MAYUMI SHINOS ...
1999 Volume 52 Issue 1 Pages
29-33
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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A new antibiotic termed zelkovamycin was isolated from the fermentation broth of
Streptomyces sp. K96-0670 by solvent extraction, ODS column chromatography and preparative HPLC. Zelkovamycin showed antibacterial activity against
Xanthomonas oryzae,
Acholeplasma laidlawii,
Pyricularia oryzae and
Staphylococcus aureus.
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II. Structure Elucidation
NORIKO TABATA, HIROSHI TOMODA, HUA ZHANG, RYUJI UCHIDA, SATOSHI OMURA
1999 Volume 52 Issue 1 Pages
34-39
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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The structure of antibiotic zelkovamycin was elucidated as a cyclic pep tide comprising glycyl, 2-aminobutanoyl, 2-ammo-2-butenoyl,
N-methyl glycyl, alanyl, 1, 3-thiazoyl, 7-methoxytryptophanyl and 2-methyldehydrothreonyl residues. The sequence of the amino acids was established by spectroscopic studies including
1H-
1H COSY,
13C-
1H COSY,
13C-
1H HMQC,
13C-
1H HMBC,
15N-
1H HMQC and
15N-
1H HMBC NMR experiments.
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ARNE HEYDORN, TRINE SUHR-JESSEN, JENS NIELSEN
1999 Volume 52 Issue 1 Pages
40-44
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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The growth and production kinetics of a teicoplanin producing strain of
Actinoplanes teichomyceticus (ATCC 31121) was investigated during batch cultivations on defined media. The growth was characterised by two exponential growth phases (EGPs), with a higher specific growth rate in the first than in the second phase. Also the specific rate of formation of teicoplanin was significantly lower in the second phase than in the first phase. This two-phased growth pattern was suggested to be caused by inhibition of growth by teicoplanin accumulated. Furthermore high concentrations of ammonia or phosphate reduced both the specific growth rate in the first EGP and the total production of teicoplanin.
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CHIKAKO ODAKA, ERIKO TOYODA, KYUICHI NEMOTO
1999 Volume 52 Issue 1 Pages
45-51
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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Deoxyspergualin (DSG) has both antitumor and immunosuppressive activities. We explored the mechanism of DSG activities using an aqueous soluble analogue, methyldeoxyspergualin (MeDSG) for
in vitro culture studies. It is known that DSG has inhibitory effects on cell proliferation, and we also observed that MeDSG inhibited [
3H]-thymidine incorporation by rapidly dividing murine T cell hybridomas. However, when tetrazolium (MTT) colorimetric assay was adopted to evaluate its inhibitory effects on cell proliferation, MeDSG induced an enhanced MTT reduction. When we examined whether these results were applicable to the actively dividing cells of other origins than T cells, similar effects were seen with Raji cells, J774.1 cells and NIH3T3 cells. N-30, another analogue which was capable of suppressing anti-SRBC antibody production
in vivo, also induced inhibition of cell growth and an enhanced MTT reduction. In contrast, the analogue which failed to prevent the antibody production, neither enhanced MTT reduction nor inhibited cell proliferation. Our results demonstrated that the ability to generate MTT formazan in dividing cells is a common property among DSG analogue with the immunosuppressive and antiproliferative activities.
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L. J. L. NORCIA, A. M. SILVIA, S. F. HAYASHI
1999 Volume 52 Issue 1 Pages
52-60
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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A systematic analysis of the bacteriostatic/bactericidal effect of several antibiotics used in veterinary medicine was carried out by time-kill kinetic analysis using
P. haemolytica,
P. multocida,
A. pleuropneumoniae, and
E. coli. The antibiotics tested were enrofloxacin, danofloxacin, erythromycin, tilmicosin, penicillin G, ceftiofur and tetracycline. Unexpectedly, the antibiotics well characterized as bacteriostatic agents against human pathogens such as tetracycline and macrolides, showed bactericidal activity against
P. haemolytica and
A. pleuropneumoniae. In contrast, tetracycline and erythromycin were bacteriostatic and tilmicosin was bactericidal against
P. multocida. In addition,
P. multocida was killed by fluoroquinolones at a slower rate than the other bacteria. Spectrum analysis revealed that ceftiofur and tilmicosin were good substrates of the universal efflux pump, AcrA/B, but penicillin and tetracycline were not. The fluoroquinolones were modest substrates for AcrA/B.
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SATOSHI OMURA, YUMI ENOMOTO, MAYUMI SHINOSE, YOKO TAKAHASHI, YUZURU IW ...
1999 Volume 52 Issue 1 Pages
61-64
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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TAKAYUKI HOSOYAMA, TAKEHIRO YUGE, SENJI TAKAHASHI, TOMOO SUZUKI, TOYOJ ...
1999 Volume 52 Issue 1 Pages
65-67
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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IV. Dihydro and Tetrahydro Desmycosin Oximes
AMALIA NARANDA, NEVENKA LOPOTAR
1999 Volume 52 Issue 1 Pages
68-70
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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TOSHIAKI SUNAZUKA, HAJIME TAKIZAWA, MASASHI DESAKI, KUNIHIKO SUZUKI, R ...
1999 Volume 52 Issue 1 Pages
71-74
Published: January 25, 1999
Released on J-STAGE: September 19, 2008
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