In the previous papers (1)(2), the extraction processes, some properties and the antitumour activities of the crude powder of carzinocidin which was produced by the strain No. 48-B-3 were recorded. In this paper, the taxonomic studies of this strain No. 48-B-3 are presented.
The strain No. 48-B-3 was isolated from the soil at Akihabara, Tokyo and found to produce a new antitumor substance, carzinocidin, in the culture filtrate and an antifungal substance both in the mycelium and in the culture filtrate which was identified to be antimycin A. (3)(4)(5)
The authors isolated also another strain No. 21-A-2 from the soil at Kitazawa, Tokyo which produced antimycin A(6) and carzinocidin. As presented in this paper, the strain No. 48-B-3 and the strain No. 21-A-2 were found to belong to a same species.
The antitumor substance, carzinocidin was somewhat resembling to carzinomycin which was reported by Dr. Hosoya, et al.(7) in thep oint of solubility and the extraction process. Thereafter, the taxonomic studies on the strains No. 21-A-2 and No. 48-B-3 (carzinocidin-prcducing strains) and S. ganmycicus (carzinomycin-producing strain) were made, and presented in this paper. Furthermore, the comparative studies on our carzinocidin-producing strains which also produced antimycin A, the antimycin-producing strain NRRL-2288(5) and the strain No. 2A-720 which was isolated by Dr. Yonehara, et al.(8) were made, and presented in this papar. The strain NRRL-2288 was delivered to the authors by the courtesy of Dr. Hesseltine, and the strain No. 2A-720 by Dr. Yonehara, Institute of Applied Microbiology, Tokyo University.
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