It has been reported in the previous paper (TORYU et al. 1960) that the glycogen-laden nuclei were found within the anterior lingual gland cells of the pullets and of the laying hens, but not within those of the baby female chicks and of the moulting hens.
In the present investigation on the male chicks of from 2 to 180 days of age, the glycogen-laden nuclei were found within the anterior lingual gland cells of the 100 days old cockerels, but not within those of the younger and older cockerels. It was also found that the structure of the gland containing the glycogen-laden nuclei became more complicated and the cells had a less amount of mucin. The occurrence of the glycogen-laden nuclei and the advanced structure of the gland may be related with the reproductive function of the chicks, possibly through the influence of gonadal or other related hormones.

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[in Japanese]

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The fresh-water fish, Aryzias latipes, was bred in two pools at temperature of 15°C and 30°C for 3, 10, 25 and 30 days, and the changes of thier liver cells were observed with the electron microscope.
1. The liver cells of the fish bred in the pool of 15°C have two parts: the darker parts of the higher electron density occupying a large part of the cell, and the clearer parts of the lower electron density. The liver cells of the fish bred in the pool of 30°C have only clearer parts.
2. The liver cells of the fish in the pool of 15°C contains little lipid granules and those in the pool of 30°C show various stages of accumulation of the lipid granules.
3. The cell substances not dissolved after the treatments of BOUIN's fluid or 5% K₂Cr₂O₇ are contained much more in the liver of the 30°C-fish. It is supposed that the substances which are related to proteins have been much more synthesized.
4. The liver cells of the 30°C-fish contain besides clear mitochondria many dark mitochondria and microbodies which are supposed to be related to the lipid contents. Also the endoplasmic reticula in alveolate and vacuolate form to which small granules of PALADE are attached are seen in almost all parts of the cell.
5. It is supposed that the tendency of growing clear in the liver cells at 30°C is caused by the increase of their surface-permeability and water contents, which is based on the acceleration of metabolism inside of the cell accompanying changes of its structure. On the clear parts of the liver cells of the 15°C-fish the small granules of PALADE and mitochondria are very few.
6. The reason why these dark parts in the liver cells of the fish bred in the pool of 15°C make their appcarance is not clear. It may be related to the water contents of the cells or may be caused by the qualitative difference of the constructive substances.

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For the lymph-node tissue culture, most works were carried out by cover-slip method with classic media and culture technics up to this time. Therefore, we have studied human lymph-node by means of clinical tissue culture as well as fluorochrominized tissue culture devised in our laboratory, and also applied these technics to clinic.
Method: 1. Clinical tissue culture. After a drop of serum was spread out on a HIRAKI's tissue culture plate (depth, 200μ), the tissue fragment is placed in the center of serum and a drop of V. B₁₂ (100γ/cc) was added. A cover slip was laid on the plate and sealed with paraffin, then, the plate was placed in a incubator.
2. Fluorochrominized tissue culture. In the medium composed of a drop of serum and a drop of Ao. -V. B₁₂ solution (Acridine orange 0.2mg/cc, V. B₁₂ 80γ/cc) the tissue frament was cultured, and observed under the fluorescence microscope with OSRAM HBO 200 mercury vapor lamp, exciter filter BG 12 and barrier filter OG 5.
Result: 1. Clinical tissue culture. The growth zone of non-pathologic lymph-node is densely packed with cells in the central part, but in the intermediate to the peripheral zones less densely. In lymphadenitis simplex, the cell growth was far superior to the non-pathologic one, while in the patients with reticulosarcoma, tuberculosis, HODGKIN's disease and cancer metastasis the cell growth were mostly poor. In acute lymphocytic leukemia, cells in the growth zone were extremely dense and the boundary is distinctly defined.
2. Fluorochrominized tissue culture.
The growth zone of non-pathologic lymph-node presented diffusely yellowish green color sparsely intermingled with reddish orange fluorescent spots. As to the pathologic lymph-node with poor cell growth, reticulosarcoma presented yellowish green fluorescence but in HODGKIN's disease fluorescence had more reddish hue, and in cancer metastasis reddish orange fluorescence was found in the explant. In acute lymphocytic leukemia, fluorescence was green in the central part of the growth zone and yellowish green at the periphery.
Conclusion: Since there were some definite differences in growth pattern and fluorescence of the respective growth zone of lymph-node tissue culture in various diseases, these culture methods are useful for the diagnosis of the disease with enlarged lymph-node.

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The fresh-water fish, Aryzias latipes was bred at a suitable temperature of 25°C and at a higher temperature of 34°C for 3-47 days. Feeding and other conditions were the same as in the preceding experiment.
1. At 25°C, the electron microscope figure of the liver cells is similar to that 15°C
2. Around at 30°C, the metabolism in the liver seems to be abnormally accelerated, but when it goes up over 30°C the function fall is observed on the contrary, because of the lowering of the water contents or of pH.
3. At the condition of starvation the liver cells become darker, which is supposed to represent a stiffening of them. Also there appear large abnormal granules which seem to have grown from varients of mitochondria and microbodies.

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The author examined the appearance of hemoglobin in the earlier cells of the erythroid series in the extravascular blood forming areas, primitive blood islands, and the blood in the primitive blood vessels at the yolk sack of chicken embryo.
Applicated techniques were 1. GIEMSA'S and MAY-GRUENWALD-GIEMSA'S stains for determinating the developmental stages of cells, 2. microspectrography to research the heme and RNA absorptions and 3. RALPH's stain method to indicate the hemoglobin.
1. Primitive blood cells indicated no heme absorption and hemoglobin activity.
2. Promegaloblast had considerable vacuoles in the cytoplasm, which showed significantly the diffusal heme absorption and hemoglobin activity. But their granulated pictures which were characteristic to the intranuclear hemoglobin were not found in the nuclei of cells at this stage. Then, hemoglobin may be chiefly produced in the cytoplasm of erythroblast.
3. The intensity of both hemoglobin activity and heme absorption were so increased in the cytoplasm of basophilic megaloblast without vacuole. In addition to it, hemoglobin appeared in the nucleus and was localized among the meshes of the chromatin net work except in the site of nucleoli.
4. The hemoglobin at the sites of chromosomes in anaphase was photographed with the similar granulated feature like those in resting phase. With all of these findings, author's results agree with THORELL's; it is supposed that most intranuclear hemoglobin may be caused by its adhering to the chromosomes at the mitotic stage.

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