Archivum histologicum japonicum
Print ISSN : 0004-0681
Volume 39, Issue 2
Displaying 1-5 of 5 articles from this issue
  • Toshihiko YAJIMA
    1976 Volume 39 Issue 2 Pages 79-97
    Published: 1976
    Released on J-STAGE: February 20, 2009
    JOURNAL FREE ACCESS
    Ultrastructural and cytochemical studies on the remodelling of the rat tracheal cartilage have been carried out. The thickness of the tracheal cartilage was constant, during the observation periods (1 to 54 days after birth). The external perichondrium of the tracheal cartilage consisted of active fibroblasts and intercellular fibrils. The inner part of this perichondrium was a chondrogenic layer, where appositional growth was taking place. On the other hand, the internal perichondrium contained fibroblast-like cells, which were nearly twice as large as the external perichondrial fibroblasts in size and were arranged in three or four layers. The cells had well developed organella and large vacuoles which contained numerous fragments of fibrils and/or glycosaminoglycan. Many cytoplasmic processes protruded to the cartilage matrix, where the intercellular fibrils were particularly irregular in arrangement. Some vacuoles included collagen fibrils. Based on an intense acid phosphatase activity in these vacuoles and other findings, the fibrils were thought to be phagocytosed collagen of the cartilage matrix. An extensive alkaline phosphatase activity was demonstrated on the plasma membrane of fibroblasts and chondroblasts in the external perichondrium.
    The present investigation revealed distinct functional difference between the external and internal perichondrium of the tracheal cartilage. It is resorbed at the internal perichondrium, while it appositionally grows at the external perichondrium. The fibroblast-like cells of the internal perichondrium play an essential role in resorption of the matrix in cartilage remodelling.
    Download PDF (20211K)
  • Takuro MURAKAMI
    1976 Volume 39 Issue 2 Pages 99-103
    Published: 1976
    Released on J-STAGE: February 20, 2009
    JOURNAL FREE ACCESS
    Small tissue pieces for scanning electron microscopy were punctured, before fixation, with a needle and perfused through the needle with Ringer solution. This puncture perfusion sufficiently eliminated blood and tissue fluid from the tissue and was of excellent value in cleaning the small samples which were hardly cannulated for vascular irrigation. Resin injection by this needle puncture method reproduced not the tissue spaces but, almost exclusively, the blood vessels.
    Download PDF (9378K)
  • Observation of Individual Ferritin Particles on Erythrocytes
    Akira HATTORI, Yutaka MATSUKURA, Suiko ITO, Tsuneo FUJITA, Junichi TOK ...
    1976 Volume 39 Issue 2 Pages 105-115
    Published: 1976
    Released on J-STAGE: February 20, 2009
    JOURNAL FREE ACCESS
    This paper comprises a part of our study to examine whether ferritin can be used as an immunological surface marker for high resolution scanning electron microscopy.
    In the first part of the study individual ferritin particles in a sample of purified ferritin were demonstrated as spheres with a diameter of about 170Å by use of fixation by glutaraldehyde and thin coating by ion sputtering.
    In the second part of this study, ferritin particles as indicating the antigen sites of blood group A were also recognized on the erythrocytes by the same techniques as in the first part. Ferritin deposition differed in amount among the erythrocytes, but without regard to the shape of the cells. On the individual erythrocytes, heavier depositions tended to occur in the concavity of the cell.
    In conclusion, ferritin is useful as a surface marker in immunoscanning electron microscopy. It has the benefit, in comparison with the other markers hitherto reported, of allowing us to observe the fine features of the cell surface under immunological reaction.
    Download PDF (13203K)
  • Yutaka TANUMA, Masako OHATA, Toshio ITO, Chihiro YOKOCHI
    1976 Volume 39 Issue 2 Pages 117-145
    Published: 1976
    Released on J-STAGE: February 20, 2009
    JOURNAL FREE ACCESS
    In 134 out of 180 perirenal fat samples (74%) derived from Japanese necropsy cases aged from 1 month to 86 years, the brown fat tissue persisted in variable amounts. Brown fat cells were classified into 6 types: Type 1 cells are fat-depleted cells filled with granular cytoplasm and are believed to be produced after oxidation of fat for heat production. Type 2 cells are small-locular cells suitable for rapid oxidation of fat droplets. Types 3 (middle-locular) and 4 (large-locular) represent fat-storage cells containing large amounts of fat. Type 5 cells are thought to be transitional forms between multilocular brown fat cells and monolocular white fat cells. Type 6 (cytoplasm-rich multilocular) cells, usually found together with Type 1 cells, are thought to be fat-depleting or -consuming cells, since in them fat droplets are reduced in number and size probably in consequence of oxidation of fat, but by contrast granular cytoplasm is increased in amount separating the individual fat droplets by thick cytoplasmic septa. The occurrence of Types 1 and/or 6 cells that has been revealed in 65 out of the total 180 samples (36%), suggests that the oxidation of fat for the thermogenesis proceeds in the brown fat tissue and that brown fat cells partially undergo fat depletion. In the present study, the thermogenesis of human brown fat tissue was suggested chiefly with regard to the occurrence of Types 1 and/or 6 cells. In the majority of perirenal fat samples from infants (1-11 months) relatively numerous Types 1 and 6 cells were frequently revealed together with Type 2 cells, suggesting rapid and active heat production in support of the view that in human infants the brown fat tissue may be thermogenetically active to maintain body temperature. In the same manner, marked ability to produce a considerable amount of heat was evidenced in brown fat tissue of children and teenagers. In younger and elderly adults the frequency of occurrence and the amount of the perirenal brown fat tissue were decreased but Types 1 and/or 6 cells could be found in 17-40% of them, infrequently together, with Type 2 cells, suggesting persistence of the thermogenic activity with occasional large heat production especially in younger adults (20-39 years). Thus, the results obtained in this study have clarified that the human brown fat tissue can respond to stimuli given to the body by oxidation of stored fat even in the latest decades of life.
    In cases of death from burning, drowning, bleeding, drug poisoning etc., numerous Types 1 and/or 6 cells were found, suggesting that an active fat oxidation would take place in brown fat tissue assumedly as the result of the raised noradrenalin level in this tissue. The so-called small cytoplasmic cells found in perirenal fats from cases of death from liver cirrhosis and other causes were assumed to be atrophic fat-depleted brown fat cells.
    Download PDF (24222K)
  • 1976 Volume 39 Issue 2 Pages 147-148
    Published: 1976
    Released on J-STAGE: February 20, 2009
    JOURNAL FREE ACCESS
    Download PDF (220K)
feedback
Top