All issues
Successor
Volume 40, Issue 1
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Masao NORITA1977 Volume 40 Issue 1 Pages 1-10
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSThe claustrum of the cat was studied to determine the extent of its projection to the cerebral cortex. Neurons in the bilateral dorsal claustrum are labeled by retrograde axonal transport of horseradish peroxidase (HRP) from injection sites of different neocortical areas with preponderance to the ipsilateral side. It is revealed that the dorsal claustrum projects to almost entire regions of the bilateral neocortex and that distribution of labeled neurons in the bilateral claustrum has topographical correspondence, rostro-caudal as well as dorso-ventral, with regard to the sites of injections in the neocortex. No neurons in the ventral claustrum are labeled.View full abstractDownload PDF (5186K) -
I. The Transport of Thiamine across the PlacentaKazuko UCHIDA, Tateo DAIMON, Vinci MIZUHIRA1977 Volume 40 Issue 1 Pages 11-22
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSIt is well known that thiamine is highly soluble in water. However, if chloroplatinic acid (H2PtCl6) is used during fixation, an insoluble, electron-dense thiamine-PtCl6 precipitate is formed. Using this method we performed electron microscope autoradiography of the placental labyrinth of rats in their 15th day of pregnancy after injection into the maternal bloodstream of 3H-thiamine, and we also measured the radioactivity of the tissues, in order to study the transport of thiamine across the placenta. Both developed silver grains and radioactivity were found in the largest amounts 30min after injection; the amounts of both declined after 2hrs and declined further after 5hrs. The majority of the developed silver grains were localized in the smooth-surfaced endoplasmic reticulum, which is generally thought to be the vehicle for the transport of materials in the cell, while in the mitochondria, Golgi apparatus, etc. the grains were few. Considering the substantial depletion of the amounts of both silver grains and radioactivity after the relatively short time of 5hrs, we conclude that the greater portion of the thiamine passes quickly across the placenta to the fetus, where it is used in the metabolism of the fetal organs, and that only a small part of the thiamine is retained in the placenta for its own metabolism.View full abstractDownload PDF (19925K) -
II. The Uptake of Thiamine by Fetal OrgansKazuko UCHIDA, Tateo DAIMON, Vinci MIZUHIRA1977 Volume 40 Issue 1 Pages 23-33
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSHaving injected 3H-thiamine into the maternal bloodstream of rats in their 15th day of pregnancy, the uptake by the fetal organs (liver and cardiac muscle) of the 3H-thiamine which had been transported across the placenta was studied by electron microscope autoradiography and by measuring the radio-activity of the tissues, using the same methods reported in the first paper of this series. In the fetal liver both developed silver grains and radioactivity were found in greatest amounts in specimens collected 5hrs after injection. Developed silver grains were abundant in the mitochondria, Golgi apparatus, rough-surfaced endoplasmic reticulum, glycogen area, and nucleus, indicating that these cell organelles take up thiamine and utilize it for the cell metabolism. In the fetal cardiac muscle, the developed silver grains and radioactivity were found in greatest amounts 2hrs after injection. The silver grains were abundant in the glycogen area, Golgi apparatus, and myofibrils, but they were especially abundant in the mitochondria. This indicates that thiamine is taken up by these organelles and employed in the cell metabolism.View full abstractDownload PDF (26269K) -
Takuro MURAKAMI, Kazuhide YAMAMOTO, Tatsuya ITOSHIMA, Shozo IRINO1977 Volume 40 Issue 1 Pages 35-40
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSSupplement of guanidine hydrochloride to the original tannic acid solution of MURAKAMI (1973) eliminated, without debilitating the conductivity effect, the vexatious treatment with different amino-acids and sucrose in the previous method (MURAKAMI, 1974). The procedures and advantages of this simplified modification are described using human spleen samples; attempt was made to elucidate the structure of arterial terminals by dissecting the specimens under the scanning electron microscope.View full abstractDownload PDF (6592K) -
Seiji SHIODA, Yoshiharu HONMA, Sumio YOSHIE, Yasuhiko HOSOYA1977 Volume 40 Issue 1 Pages 41-49
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSThe third ventricular wall and its adjacent region of the arctic lamprey, Lampetra japonica, were studied by scanning electron microscopy to elucidate their surface fine structures. The specimens were caught in the mouth of the river during their anadromous migration.
The ventricular wall is covered entirely with the cilia of ependymal cells, with the exception of the ventral side of the lateral wall, the floor of the recessus infundibuli and a portion of the recessus posteriosus. In the ependymal layer covering the ventral side of the lateral wall, numerous protrusions of neurons are found equipped with microvilli and cilia. These neurons seem to correspond to the liquor-contacting neurons. Ependymal cells identified as tanycytes occur in the posterior portion of the floor of the recessus infundibuli. The apex of the tanycyte is provided with numerous microvilli and a bundle of cilia, while its basal projection extends towards the outer layer of neurohypophysis to make contact with the capillary wall. A small spherule considered to be a secretory substance is observed near the root of the ciliary bundle. The recessus posteriosus consists of a layer of ependymal cells and neurons with an apical projection into the ventricular cavity. Possible intraventricular macrophage (Kolmer cell) is found in the lamprey.View full abstractDownload PDF (12540K) -
Hiroyuki MIYAGAMI, Yozo WATANABE, Yoshiaki SAWADA, Koichi KATO, Kiyosh ...1977 Volume 40 Issue 1 Pages 51-62
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSA combined, electron microscope and radioimmunoassay investigation was carried out on the G cells and blood gastrin levels in patients who received either selective vagotomy (SV) plus pyloroplasty (P) or selective vagotomy plus antrectomy (A) for duodenal ulcer.
1. After SV+P, G cells did not show any degenerative or hypofunctional signs in their fine structure, but their secretory granules trended towards the cell base, suggesting a presecretory state. Six months postoperatively, the G cells were markedly increased in number (hyperplasia). In accordance, basal gastrin levels were elevated (hypergastrinemia). After SV+A, which caused better prognosis, blood gastrin levels dropped more or less conspiciously as the main source of G cells had been removed.
2. G cells denervated by surgery (SV+P), reacted to the stimuli of meat extract by releasing their granules and elevating blood gastrin levels, as the innervated G cells do. The response of G cells to the luminal stimuli is thus not believed to be essentially related to nerves.
3. The denervated G cells also could, as innervated ones, release gastrin in response to insulin hypoglycemia. Thus it is proposed that this stimulation is not mediated by the vagal nerve.
4. Gastrin release from the G cell was evidenced in this study as emiocytotic granule release from the cell base. The frequency of the Ω-shaped granule openings found in the electron microscope well corresponded to the gastrin levels in the blood.View full abstractDownload PDF (16216K) -
Toshitaka AKISAKA, Masatoyo ODA1977 Volume 40 Issue 1 Pages 63-72
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSThe localization of Mg++ activated adenosine triphosphatase (ATPase) activity in rat fungiform taste buds was demonstrated by electron microscopic histochemistry. Reaction product was found on the cell membrane of the taste cells, but not within their cytoplasm. Especially strong reaction occurred in the taste pore. Besides, enzymes were observed in axon-Schwann cell and axon-type I cell interspace, but not in the contacts of the nerve fiber and type II cell. The taste bud could well be distinguished from the surrounding epithelium by the existence of the reaction product. ATPase activity was absent at the synaptic portion where type III cells faced nerve fibers. No reaction product was seen along the basal lamina. In fungiform buds, the nerve fibers were frequently shown to make contact with each other, and these sites were devoid of deposits for ATPase.View full abstractDownload PDF (16522K) -
1977 Volume 40 Issue 1 Pages 73-75
Published: 1977
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSDownload PDF (252K)
- |<
- <
- 1
- >
- >|