Archivum histologicum japonicum
Print ISSN : 0004-0681
Volume 45, Issue 5
Displaying 1-9 of 9 articles from this issue
  • Christiane AYER-LE LIEVRE, Josiane FONTAINE-PERUS
    1982 Volume 45 Issue 5 Pages 409-427
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The neural crest cells give rise to a large variety of derivatives including neural, mesenchymal, APUD and/or paraneuron cell types.
    A better knowledge of these derivatives was partly achieved through studies using LE DOUARIN's quail/chick marker system.
    We review here evidences which were thus provided for a neural crest origin of calcitonin containing cells, carotid body, aortic paraganglia, adrenomedulla, and against a neurectodermal origin of enterogastric and respiratory tract endocrine cells.
    The role of neural crest cells in PEARSE's APUD system is discussed. The results implicate that an explanation for the common properties of these cell types and their pathological and biochemical significance should not be looked for in a common embryological origin but at another level.
    The place of neural crest and, more generally, neurectoderm derivatives in the paraneuron concept of FUJITA is examined. The relevance of the epithelial origin of these cell types to their “receptosecretory” function is stressed.
    Considering neural crest itself as a unique system is still questioned and discussed here. Its ubiquity and penetration of other systems is pointed out as a widespread phenomenon which is not restricted to APUD and paraneuron systems.
    Download PDF (14831K)
  • Nobuyuki KARASAWA, Yukari KONDO, Ikuko NAGATSU
    1982 Volume 45 Issue 5 Pages 429-435
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    By the immunoenzymatic PAP (peroxidase antiperoxidase) technique and immunofluorescent methods, the localization of a catecholamine-synthesizing enzyme, tyrosine hydroxylase (TH), in dopaminergic chief cells was elucidated in the dog and bat carotid bodies. Two types of cells were identified by PAP electron microscopy; TH-positive and TH-negative cells. The TH-positive reaction was observed both in the granules (about 170 and 300nm in diameter) and in the ground substance, but not in the nucleus. Dopamine-β-hydroxylase (DBH) reaction was negative in both cells. These findings may strongly suggest the presence of tyrosine hydroxylase not only in the cytoplasmic matrix but also in the granules in the dopaminergic cells of the carotid body.
    Download PDF (7314K)
  • Yoko KAMEDA
    1982 Volume 45 Issue 5 Pages 437-448
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The C cell complexes in the dog thyroid are the remnants of ultimobranchial bodies which retain fetal characteristics, and contain cyst structures showing various sizes, shapes and luminal contents. This study deals with these cysts with regard to their morphology, immunoperoxidase reactions to anti-calcitonin, anti-19S thyroglobulin and anti-C-thyroglobulin antisera, and ability to incorporate radioiodine. Small cysts lined by densely packed small cells are devoid of a stainable substance in their lumina and show no reaction to any of the antisera. They are regarded as immature structures. The large cysts, covered mainly by a single layer of cuboidal cells, store varying amounts of secretory products in their lumina. The secretory products showing colloid-like, flocculent, or granular features are intensely stained with the anti-19S thyroglobulin and anti-C-thyroglobulin antisera. However, they scarcely accumulate silver grains after injection of Na125I. C cells are distributed in the cyst epithelium but the cystic contents reveal no immunoreaction for calcitonin. In conclusion, the present study indicates that the cyst structures in C cell complexes synthesize and store a thyroglobulin-like glycoprotein but are not directly involved in thyroid hormone synthesis.
    Download PDF (18515K)
  • B. R. MAITI, Subrata CHAKRABORTY, Asok BORAL
    1982 Volume 45 Issue 5 Pages 449-452
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    A histological investigation was made to elucidate whether mammalian (ovine) prolactin can induce mitotic activity in pancreatic islet cell (in splenic lobe) of chicks. Prolactin treatment in different dosages (total dosage: 15, 50 and 100 IU per bird in 10 days) increased the mitotic frequency and also caused hypertrophy in the AF-positive cells of the B-islets in the splenic lobe. The increase in mitotic frequency was uniform for all doses applied. It is suggested that prolactin has a mitogenic action on the pancreatic islet cells of male chicks and that action is possibly not dose-dependent.
    Download PDF (2321K)
  • Yutaka TANUMA, Masako OHATA, Toshio ITO
    1982 Volume 45 Issue 5 Pages 453-471
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The liver of the flatfish, Kareius bicoloratus was observed by transmission electron microscopy.
    The hepatocytes surround the bile canaliculi and are loosely disposed with few connections in between. The architecture of the hepatic tissue is first supported by junctional complexes between hepatocytes abutting on the bile canalicular lumen, second, by intraparenchymal bile ductules connecting with hepatocytes through desmosomes and third, by particular “desmosomal complexes” composed of numerous desmosomes arranged along the interhepatocytic space and accompanied by bundles of microfilaments in the cells. The fourth, and most important supporting factor of the hepatic tissue, consists of sinusoidal cells including adjacent hepatocytes connected by many desmosomes.
    The Disse's space lacks collagen fibrils but contains filaments of type IV collagen. Sinusoidal cells are reinforced by abundant microfilaments in their cytoplasm. Desmosomes occur between endothelial cells, which are further connected by other desmosomes to hepatocytes as well as Ito cells. Desmosomes are most numerous around the Ito cells. Thus, the cells forming the sinusoidal wall are interconnected by many desmosomes, forming as a whole a strong framework which supports the hepatic tissue.
    The desmosomes between the sinusoidal cells are characterized, except for those between endothelial cells the mselves, by the presence of zones of microfilaments on both of their sides which extend parallel to the desmosomal plaques and are believed to strengthen the desmosomal connection.
    No Kupffer cells could be identified in the sinusoid, but macrophages were demonstrated in the parenchyma near the Disse's space.
    Download PDF (34010K)
  • Tatsuo FUJITA, Makoto SUGIMURA, Yoshitaka SUZUKI
    1982 Volume 45 Issue 5 Pages 473-481
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The migration of macrophages in chick bone marrow was studied by histoplanimetry after an intravenous injection of colloidal carbon or iron-dextran. In the initial stages after the injection, carbon-laden macrophages were found only in the sinus, where erythrocytopoiesis took place. The carbon-laden macrophages then passed through the sinus wall and gradually migrated from the sinus to the extravascular area (i. e., the granulocytopoietic region) peaking at 1 to 3 days after injection. After 7 days, almost all the carbon-laden macrophages accumulated to form “macrophage islets” mainly around the lymphatic nodules in the extravascular area. In the cases injected with iron-dextran, the movement of iron-laden macrophages was similar to that of the carbon-laden ones. The similarity suggests that the direction and time of migration of macrophages are independent of ingested materials.
    Download PDF (14802K)
  • Sato HONMA, Kazuhiro ABE, Takashi ITO
    1982 Volume 45 Issue 5 Pages 483-494
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    Free cells in the pleural cavity of the mouse were studied by quantitative and qualitative morphological procedures.
    The size distribution curve of the cells, obtained by a Coulter counter and channelyzer, indicated that the pleural free cells were composed mainly of two populations, which were morphologically classified into small and large types. The small cells were about twice as numerous in females as in males, and the large type of cells exhibited no significant difference in number between the two sexes. Therefore, the total number of pleural cells was significantly greater in females than in males.
    The small pleural cells were similar in cytological features to the peritoneal mediumsized mononuclear cells described in previous papers (ABE et al., 1979a, b) with the exception of their size. Typical small lymphocytes were also included among the small type of pleural cells, but they were very few in number. The large type pleural cells were macrophages. The pleural macrophages were somewhat variable in cell features, particularly in surface configuration, as compared with peritoneal macrophages.
    Download PDF (14879K)
  • Takehiko HIDA, Nobuo SHIMIZU
    1982 Volume 45 Issue 5 Pages 495-504
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    SATOH et al. (1978a) demonstrated that Barrington's nucleus in the rat corresponds to the nucleus laterodorsalis tegmenti (TLD), immediately ventromedial to the rostral pole of the locus coeruleus (LC). To confirm this finding and to identify the course of the tracts and their terminals, we injected small amounts of HRP into the TLD, LC and the lumbar and sacral segments followed by the DAB and TMB reaction.
    Injection of HRP into the TLD resulted in the anterograde staining of descending fibers in the latero-ventral part of the lateral funiculus, which mainly terminate in the intermediate area of the sacral segments, and in the retrograde labeling of a large number of cells in the intermediate area of the sacral segments. After injection of HRP into the sacral segments, marked labeling of many TLD cells was demonstrated in contrast to that of only a small number of LC cells. On the other hand, after injection of HRP into the LC, a moderate number of descending fibers were found in the ventral part of the lateral funiculus, with small numbers in the apex of the dorsal horn and ventral funiculus, mainly ending in the intermediate gray of the lumbar segments. Moreover, moderate numbers of retrogradely labeled cells were revealed in the intermediate area of the lumbar segments. From these results it may be concluded that the reciprocal interrelation between the TLD and intermediate gray of the sacral segments is more important for the micturition reflex than that between the LC and lumbo-sacral segment.
    Download PDF (9919K)
  • Kikuko IMAMOTO, Ryota FUJIWARA, Toshisaburo NAGAI, Toshihiro MAEDA
    1982 Volume 45 Issue 5 Pages 505-518
    Published: 1982
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    Following an intravenous injection of a fluorescent dye, primuline, into neonatal rats, the distribution of ameboid cells and their fate in the central nervous system were examined under incident fluorescence, light and electron microscopes. In the ameboid cells labeled with primuline, the ingested fluorogen spread throughout the cytoplasm, in which it appeared as a whitish yellow fluorescence.
    Such labeled cells displayed increased acid phosphatase (ACPase) activity. Combining primuline labeling with the ACPase reaction allowed for the identification of ameboid cells even after the transformation mentioned below.
    Ameboid cells were distributed throughout the white matter in neonatal rats, but were few in the gray matter. Particularly, they appeared as cell clusters in the corona radiata of the corpus callosum, the subependymal layer surrounding the ventricles, the fornical commissure, the internal and external capsules, the cerebellar peduncles and the medulla. Around 8 to 14 days after birth, the ameboid cells which had so far appeared spherical became gradually elongated with a few branched cytoplasmic processes, and scattered in the white matter, Thereafter, most primuline labeled cells transformed into cells with a dark nucleus and a thin cytoplasm including small phagosomes, i. e., into cells with features of microglia. However, a small number of ameboid cells underwent degeneration.
    The data indicate that the ameboid cells observed in the white matter during the postnatal period transform into microglia. Since other investigations suggest that the ameboid cells are derived from blood monocytes, microglia could initially come from monocytes.
    Download PDF (19507K)
feedback
Top