巻号一覧
47 巻 (1984)
- 5 号 p. 459-
- 4 号 p. 345-
- 3 号 p. 239-
- 2 号 p. 121-
- 1 号 p. 1-
47 巻, 5 号
選択された号の論文の10件中1～10を表示しています
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片岡 勝子, Yumiko SAKANO, Junko MIURA1984 年 47 巻 5 号 p. 459-474
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーHistogenesis of the mouse gastric mucosa and the distribution of epithelial cells capable of proliferation were studied by light microscopy, autoradiography with 3H-thymidine and electron microscopy. The formation of the gland begins on day 14 of gestation, while morphological signs of epithelial cell differentiation begin on day 15. The cell types include surface mucous, primitive chief and parietal cells during the late prenatal and first 2 weeks of postnatal development. Immature surface mucous cells and undifferentiated cells in the lower part of the foveola and the isthmus, and primitive chief cells throughout the gland become 3H-thymidine labeled. In addition, surface mucous cells in the superficial epithelium are labeled in fetuses and neonates several hours after birth. By 21 days after birth, primitive chief cells are replaced by chief and mucous neck cells. At that time, immature surface mucous and undifferentiated cells in the isthmus, and mucous neck cells in the upper part of the neck are radio-labeled and form the generative cell zone. The mucosa attains its full thickness by 6 weeks of age.
Immature parietal cells rarely incorporate 3H-thymidine during their development. It is suggested that some of the parietal cells may be derived from actively proliferating precursors, i. e., primitive chief cells and mucous neck cells in developing and adult animals, respectively.抄録全体を表示PDF形式でダウンロード (31472K) -
谷口 豊1984 年 47 巻 5 号 p. 475-483
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーA corticotropin-releasing factor (CRF), Oxytocin and arginine-vasopressin were localized immunohistochemically in the paraventricular nucleus of colchicine-treated male rats. Small CRF-containing neurons were distributed in the parvocellular region. Some magnocellular neurons were also immuno-stained with anti-CRF serum, but after preabsorption of the antiserum with pure synthetic CRF, the staining of these magnocellular neurons was unaffected, which suggests that this CRF-positive reaction was non-specific. By comparing the distribution of CRF and oxytocin or vasopressin on serial 5μm cryostat sections, it was revealed that CRF and oxytocin or CRF and vasopressin do not coexist in the same neuron.抄録全体を表示PDF形式でダウンロード (12526K) -
山本 正夫, Katsuko KATAOKA1984 年 47 巻 5 号 p. 485-493
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーIntercellular junctions were studied in pancreatic islets of some rodents by electron microscopy of thin sections and freeze-fracture-replicas. Junctions between islet cells can be classified into three types: macula occludens, a combination of the macula occludens and gap junction, and gap junction. The first and second types are mainly located on the cell membrane near the intercellular canaliculus, while the third type is present independently of it. The development of these types of junctions varies considerably among animal species: in mice, guinea pigs and hamsters, the second and third types are frequent, but the first type is rarely seen. In Mongolian gerbils, diminutive elements of the three types are infrequently present. In rats, only the third type is developed.
In addition to intercellular communication through gap junctions, the first and second types of junctions may incompletely discriminate the intercellular canalicular lumen, and regulate the microenvironment of the islet cell.抄録全体を表示PDF形式でダウンロード (14387K) -
平 一男, Kikuo YASUNO, Susumu SHIBASAKI1984 年 47 巻 5 号 p. 495-503
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーThe basolateral plasma membrane of the gastric parietal cells in rats after food-deprivation and food-restitution was studied by thin-section and freeze-fracture electron microscopy. A decrease in the number of tubulovesicles and dilatation of the canalicular lumen were generally observed in the parietal cells of the refed rats. Basal folds, demonstrated both in fasting and food-resupplied rat groups parietal cells by thin-section electron microscopy, appears as branched furrows on the P face and branched wrinkles on the E face plasma membrane by the freeze-fracture replica method. Parietal cells providing numerous basal folds appeared to be more numerous in refed rat gastric gland. The basal folds may represent, at least to some extent, a reservoir of a surplus plasma membrane and play an important role in secreting substances into and also absorbing substances from the blood capillary via the underlying connective tissue.抄録全体を表示PDF形式でダウンロード (17068K) -
星 素, Kaeko KAMIYA, Ritsuko TAKEMOTO, Hiroshi AIJIMA1984 年 47 巻 5 号 p. 505-518
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーChanges in the number of lymph follicles and germinal centers in draining popliteal lymph nodes were investigated in 8-week-old mice injected with either phytohemagglutinin (PHA) or lipopolysaccharide (LPS) into the footpad of the left hind leg. The dose of PHA injected ranged from 10μg to 1mg, and that of LPS, from 2 to 200μg. In unstimulated animals, the popliteal lymph nodes contained only a small number of germinal centers, and many of the lymph follicles in the nodes were in the form of primary follicles. In the draining lymph nodes, regardless of the dose injected, PHA induced germinal center development in existing primary follicles, but eventually failed to stimulate the formation of new lymph follicles and germinal centers. On the other hand, LPS not only induced germinal centers in the existing follicles, but also stimulated the formation of new primary follicles, many of which then developed germinal centers. The occurrence of new follicles in the LPS-treated lymph nodes was dose-dependent, and LPS appeared to cause de novo formation of follicles.抄録全体を表示PDF形式でダウンロード (15524K) -
遠山 稿二郎, Chizuka IDE1984 年 47 巻 5 号 p. 519-532
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーThe localization of laminin and fibronectin was examined on the basal laminae of Schwann cells. Basal laminae from sciatic nerves were isolated by sonication, and the localization of laminin and fibronectin on such isolated basal laminae was studied by immunoferritin histochemistry. Laminin was localized mainly on the cellular side (i. e. the side originally facing the Schwann cell plasma membrane) of the basal laminae. On the other hand, fibronectin was found to be present as aggregates only on the interstitial side (i. e., the side originally facing the endoneurial connective tissue) of the basal laminae. Thus, the locations of laminin and fibronectin were distinctly different. It is presumed that laminin might be involved in the attachment of axons and Schwann cells to the basal laminae, while fibronectin mediates the adhesion of the basal laminae to connective tissue elements, including the collagen fibrils. These findings are discussed from a standpoint of nerve regeneration through the basal laminae scaffolds of Schwann cells.抄録全体を表示PDF形式でダウンロード (20437K) -
緒方 卓郎, Yuichi YAMASAKI1984 年 47 巻 5 号 p. 533-539
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーThree-dimensional structures of the Schwann cell in the rat motor endplate were observed by scanning and transmission electron microscopy. A few Schwann cells lodged over the surface of the motor endplates and their nuclear portion swelled roundly. From the Schwann cell body, a few processes extended downwards, divided several times and covered only the upper orifice of the synaptic groove; they did not cover the surface of the protuberances or the ridges of the sarcolemma which separated the synaptic grooves. The lateral sides and ends of the Schwann cell processes were frilled with finger-like projections. They fixed on the surface of the basal lamina of the sarcolemma and sealed the nerve terminals in the synaptic grooves.抄録全体を表示PDF形式でダウンロード (14168K) -
Katsushi NAKAMURA, Nobuo OHTA, Wataru KAWAJI, Kuniaki TAKATA, 平野 寛1984 年 47 巻 5 号 p. 541-547
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーChanges in permeability of adult rabbit articular cartilage caused by joint contracture were studied by light and transmission electron microscopy, employing horseradish peroxidase (HRP) as an indicator. The knee joint was plaster-immobilized for 0, 2, 4, 6, or 8 weeks in the flexion position. One ml of 4% HRP was administered in the articular cavity of the knee joint and allowed to diffuse and permeate into the articular cartilage. Distribution of the permeated HRP was visualized in the cartilage taken from the lateral condyle of the femur, utilizing the DAB-H2O2 reaction. In the normal and the non-immobilized joints, the permeated HRP reached to the matrix and chondrocytes situated in the deep layer of the articular cartilage. HRP was heavily deposited in the intercellular matrices, particularly around the chondrocytes, and was actively endocytosed by these cells. In the plaster-immobilized joints, especially after 4 weeks or longer of immobilization, the administered HRP had not permeated well and was restricted to the surface (lamina splendens) and the superficial layer of the cartilage. These results show that administered HRP diffuses into the deep layer of the articular cartilage and is actively endocytosed by chondrocytes and that the permeability of articular cartilage is remarkably reduced by joint contracture.抄録全体を表示PDF形式でダウンロード (8735K) -
町野 満夫, Hiroyuki MORIOKA, Masayoshi TACHIBANA, Takashi TSURUOKA, Osamu ...1984 年 47 巻 5 号 p. 549-551
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーUsing a protein A-gold immunohistochemical technique, the lysozyme rich core and the lysozyme free peripheral rim were differentiated in the secretory granule of the serous cell in the human nasal mucosa, under the electronmicroscope. The shperical lysozyme rich core, which had been excreted into the gland lumen, was also wrapped with the peripheral rim. This finding suggests that the peripheral rim defends the integrity of the core and inhibits the release of lysozyme from the core.抄録全体を表示PDF形式でダウンロード (3073K) -
牛木 辰男, Katsuhisa YONEHARA, Toshihiko IWANAGA, Tsuneo FUJITA1984 年 47 巻 5 号 p. 553-557
発行日: 1984年
公開日: 2011/10/26
ジャーナル フリーA backscattered electron (BSE) image was applied to the observation of freeze-cracked tissue blocks stained with the indirect immunoperoxidase method. On the cracked surface of the pancreas, the BSE image clearly revealed the immunostained B cell granules as bright spots. A pair of secondary electron and BSE images on a fractured surface are useful for the three-dimensional localization of antigens in cells.抄録全体を表示PDF形式でダウンロード (5761K)
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