Archivum histologicum japonicum
Print ISSN : 0004-0681
Volume 49, Issue 5
Displaying 1-9 of 9 articles from this issue
  • Bela VIGH, Ingeborg VIGH-TEICHMANN
    1986 Volume 49 Issue 5 Pages 495-518
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The pineal complex in frogs (Rang esculenta, R. temporaria, R. tigrina, R. arvalis) was studied by conventional electron microscopy and postembedding rhodopsin immunoelectron microscopy. Three types of photoreceptor cells were found in both the pineal and frontal organs. In the pineal organ, most of the photoreceptors exhibited rhodopsin-immunoreactive outer segments and large inner segments with a large ellipsoid of densely packed mitochondria (“rod-like” photoreceptors). A small number of photoreceptors was rhodopsin-immunonegative (“cone-like” photoreceptors). In both Rana esculenta and R. temporaria, the latter were either supplied with an oil droplet and an ellipsoid in their inner segment, or they were electron-lucent with a small inner segment without an ellipsoid. In contrast, the frontal organ displayed many immunonegative “cone-like” outer segments and few rhodopsin-immunoreactive “rod-like” photoreceptors. In both organs, the basal processes of the photoreceptor cells were found to form ribbon-containing axonal pedicles which synapsed with the dendrites of secondary neurons. The latter rarely received any further afferences by conventional synapses.
    The frog pineal organ is considered a predominantly “rod-type” and the frontal organ a “cone-type” photosensory organ. The presence of three kinds of pineal/frontal photoreceptors is discussed in connection with the occurrence of different photopigments (rhodopsin/porphyropsin, iodopsin, ultraviolet and/or blue pigments) enabling the animal to discriminate by the pineal complex environmental light in various ranges of the spectrum.
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  • Yasuko TAKEOKA, Katsuko KATAOKA
    1986 Volume 49 Issue 5 Pages 519-534
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The distribution of proliferative cells and maturation of epithelial cells were studied in the pyloric mucosa of developing mice by 3H-thymidine autoradiography, carbohydrate histochemistry and electron microscopy.
    The formation of the gastric foveola and pyloric gland were seen to begin as an invagination in the epithelial surface and/or the formation of intraepithelial cavity on day 13 of gestation (day E13). Surface mucous cells and pylorocytes were first identified on day E16 by carbohydrate staining as well as by their fine structure. Both types of cells rapidly acquired abundant membranous organelles and secretory granules within the first postnatal day, maturing in fine structure by day 28.
    Proliferative cells were distributed over the epithelium by day E15, while they were rarely found at the mucosal surface after day E16. Concomitantly with the elongation of foveolae and glands during postnatal development, the proliferative capability of surface mucous cells diminished from the foveolae and that of pylorocytes from the glands, respectively; the generative zone was restricted to the isthmus by day 21, as in the adult animal.
    These results reveal that the histogenesis of the mouse pyloric mucosa is accomplished by the end of the weaning period.
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  • Kazunobu SASAKI, George MATSUMURA
    1986 Volume 49 Issue 5 Pages 535-541
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    Circulating erythroblasts of embryonic mice were karyometrically examined by light microscopy.
    In the erythroid cells in embryonic blood vessels, mitoses were encountered from 9 to 12 days of gestation. At 9 days, the circulating blood cells consisted of proerythroblasts and less mature cells. The nuclear diameter ranged from 4.8 to 9.8μm, the majority ranging between 6 and 8μm. At 11 days, hemopoietic cells with a nuclear diameter larger than 6μm disappeared from embryonic circulation, and more than 90% had a nuclear diameter of less than 5μm. Between 12 and 16 days of gestation, the smallest orthochromatic erythroblasts measuring 3.4μm nuclear diameter showed the highest peaks.
    The progress of primitive erythropoiesis in embryonic circulation is discussed in comparison with that of definitive erythropoiesis.
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  • Aiji OHTSUKA, Takuro MURAKAMI
    1986 Volume 49 Issue 5 Pages 543-552
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    Ferric chloride, when boiled with ammonium thiocyanate, ammonia and cacodylic acid, is converted into a fine anionic iron colloid which consists of 1-1.5nm electron dense granules and gives a distinct Prussian blue reaction. This colloid allows light and electron microscopic detection of ionized cationic sites in tissues at a pH range of 4.0-9.0. Light and electron micrographs of the rat kidney stained with this colloid are demonstrated. These micrographs indicate that the foot processes of glomerular podocytes and the brush border of the proximal convoluted tubule contain positively-charged groups in their intracellular matrices, and that the foot process plasma membrane fronting the Bowman's capsular space has positively-charged groups. The latter finding, together with our previous study (MURAKAMI et al., 1986), suggests the co-existence of cationic and anionic sites on this foot process surface.
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  • Tatsuo USHIKI, Chizuka IDE
    1986 Volume 49 Issue 5 Pages 553-563
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The endoneurium of the mouse sciatic nerve was studied by scanning and transmission electron microscopy to elucidate their three-dimensional architecture.
    The endoneurium consisted of collagen fibrils, and occasional fibroblasts and blood vessels. Collagen fibrils surrounded individual nerve fibers, forming two distinct layers of connective tissue sheath: the outer one was composed of bundles of longitudinally oriented collagen fibrils and the inner one was of a delicate network of interwoven thin collagen fibrils. These outer and inner layers of collagen fibrils correspond to the two fibrous sheaths known as the sheath of Key and Retzius and the sheath of Plenk and Laidlaw, respectively, revealed on nerve fibers by silver impregnation. Some of the finest collagen fibrils forming the inner network are closely attached to the basal lamina of Schwann cells, suggesting that these fibrils are concerned with the connection between the basal lamina and the inner collagen network. These two layers of collagen fibrils were found on all the nerve fibers, suggesting that they represent a general structure ensheathing the peripheral nerve fibers. Although these layers occur also on unmyelinated nerves, they are most developed on the largest myelinated fibers.
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  • Katharina SPANEL-BOROWSKI, Gerhild SOHN, Werner SCHLEGEL
    1986 Volume 49 Issue 5 Pages 565-577
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    This study was conducted to examine follicle growth and intra-ovarian oocyte release in rabbit ovaries influenced by a local application of prostaglandin formation inhibitors. While five rabbits remained as unstimulated controls, twenty-seven mature rabbits were hyperstimulated by pure follicle stimulating hormone (FSH) followed by human chorionic gonadotropin (hCG) at time 0. At the same time, 1mg lonazolac (lonazolac group), indomethacin (indomethacin group) or saline (so-called gonadotrophic stimulated controls) was applied into the vagina of each group. Both ovaries of each animal were evaluated morphometrically at 15, 20, 28 and 48hr after hCG. The absolute numbers of smaller-sized preantral follicles were always higher in the gonadotrophic stimulated controls than in the unstimulated controls, the lonazolac, or the indomethacin group. The numbers of larger-sized preantral and antral follicles showed no differences. Unlike the gonadotrophic stimulated controls and the lonazolac group, the indomethacin group displayed an increase in its mature structures (large-sized antral and preovulatory follicles, follicle and luteinized cysts) from 20 to 28hr after hCG. Call-Exner bodies underwent pseudomucification in preovulatory follicles. The absolute numbers of Call-Exner bodies were augmented by indomethacin. Maturation division of oocytes began to decrease in all three treated groups between 15 and 20hr post hCG, but the height of intra-ovarian oocyte release (IOR) appeared at 28hr. This coincided with a striking thrombus formation. It is assumed that: 1) lonazolac and indomethacin inhibit early development of proliferating follicles; 2) lonazolac and indomethacin have no effects on the maturation division of the oocyte and of IOR; 3) IOR extends over a longer period after ovulation induction in contrast to the maturation division of the oocyte; and 4) thrombus formation is related to IOR.
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  • Koichi IIJIMA, Kazuo OHTOMO, Ryoji KOBAYASHI, Naosuke KOJIMA
    1986 Volume 49 Issue 5 Pages 579-591
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    GABAergic neuronal profiles in the supraoptic nucleus of the rat were immunohistochemically identified by using a purified GABA antibody with the peroxidase-antiperoxidase method. The localization of GABA-like immunoreactivity in nerve terminals on the neurosecretory neurons was examined electron microscopically.
    A few small GABAergic neurons were found inside the supraoptic nucleus while only a very few medium-sized ones were detected in the perinuclear zone. Intrinsic, non-GABAergic small neurons covered by GABAergic neuropil were also detected. The neuropil with GABAergic axo-somatic synapses evenly encompassed unlabeled neurosecretory perikarya throughout the supraoptic nucleus. The GABAergic system seems to inhibit both vasopressin and oxytocin cells. In this area, glia cells showed clear outlines of unlabeled somata around counter-stained nuclei. Blood capillaries in the supraoptic nucleus were only slightly covered with a GABAergic neuropil.
    Electron microscopic observations demonstrated the presence of GABAergic axo-somatic symmetrical and axo-dendritic asymmetrical synapses on the neurosecretory neurons. GABA-like immunoreactivity was localized on the membranes of microtubules and synaptic vesicles, on the external membranes of the mitochondria, and on the inner leaf of the presynaptic sites. Numerous pairs of non-immunoreactive synapses were arranged along these immunoreactive synapses.
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  • Takanori DOMON, Minoru WAKITA
    1986 Volume 49 Issue 5 Pages 593-602
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    Osteoclasts of rat mandibulars were observed under the transmission electron microscope with the aim of understanding the three-dimensional structure of the ruffled border. After observation, the same block was remounted to obtain sections of the same osteoclasts at right angles to the first sectioning plane. The structure of the ruffled border of osteoclasts was observed in the two perpendicular directions.
    The ruffled border of osteoclasts was found to consist of two areas: one being composed of finger-like processes and the other, of plate-like processes. The distribution of the two areas of processes in the ruffled border did not show any apparent regularity. Not all processes continued to the cell body; some processes (stem processes) did while others were interwoven branches of the stem processes. The finger-like processes were long and rod-shaped and a few of them branched directly from the stem processes. The plate-like processes were long and belt-like and showed complicated branching from the stem processes; some of these were possibly the long belt-like processes that were complicatedly folded up to make many secondary plates; they are arranged parallel to each other in a given area. The relationship between the structure and function of the ruffled border is discussed.
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  • Shunichi ROKUKAWA, Kinji INOUE, Kaoru MIYAMOTO, Kazumasa KUROSUMI, Mas ...
    1986 Volume 49 Issue 5 Pages 603-611
    Published: 1986
    Released on J-STAGE: October 26, 2011
    JOURNAL FREE ACCESS
    The immunohistochemical localization of inhibin in porcine and bovine ovaries was studied, using monoclonal antibodies to bovine follicular fluid 32K inhibin (bFF 32K inhibin) and a polyclonal antiserum to porcine follicular fluid 32K inhibin (pFF 32K inhibin). In order to obtain a precise immunohistochemical staining, various fixations were tested with an immunoblotting procedure. Acetone fixation followed by celloidin embedding proved to be most appropriate for the immunohistochemical study of inhibin. A consistent pattern emerged in all sections prepared from porcine and bovine ovaries. Granulosa cells were specifically stained by the antibodies, and especially the cells constituting the inner layer were more intensely stained than the cells close to the theca.
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