A field vaccination was made against red spot disease (
Pseudomonas anguilliseptica infection) of eels. About 2, 000 eels (
Anguilla japonica) were vaccinated by 2 injections with heated
P. anguilliseptica bacterin and another 2, 000 eels were vaccinated by immersion with a sonicated bacterin. Eels were kept in a commercial pond with 3, 000 sham vaccinated eels for about 10 months during wintering and growing periods.
By monitoring the agglutinin titers throughout the experimental period and resistance of groups to the artificial challenges, the injected vaccine produced a persistent and protective immunity in the eels. On the other hand, the fish vaccinated by immersion did not develop agglutinating antibodies or protection.
The difference in survival rate of the eels determined at the termination of the experiment also showed efficacy of the injection vaccination, though it was not significant.
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