Aquaculture Science
Online ISSN : 2185-0194
Print ISSN : 0371-4217
ISSN-L : 0371-4217
Volume 35, Issue 3
Displaying 1-11 of 11 articles from this issue
  • Daisuke FUJITA
    1987 Volume 35 Issue 3 Pages 135-138
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
  • Daisuke FUJITA
    1987 Volume 35 Issue 3 Pages 139-141
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
  • Ken-ichi YAMAMOTO, Makoto HOSOMOTO, Tatsuya KAMIMURA
    1987 Volume 35 Issue 3 Pages 143-146
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
    Oxygen consumptions in the red porgy, the banded grouper, the file fish, the multicolorfin rainbowfish and the frogfish under progressive hypoxic condition kept the same levels as those under normoxic condition, but gradually decreased at below 50, 25, 40, 50 and 16% in oxygen saturation, respectively. Three in fortyfour fish of red porgy and seventeen in forty-nine fish of file fish were suffocated to death at 30 and 16% in oxygen saturation, respectively. But other fish were not done to death at 10%.
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  • Taizo SAKATA, Nobuyuki TARUNO
    1987 Volume 35 Issue 3 Pages 147-151
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
    The generic compositions of microf lora associated with alimentary tract and integument of cultured prawns, Penaeus japonicus, were compared among various stages of post larvae and adult. Flavobacterium and Pseudomonas were the predominant genera isolated from the alimentary tract of the early stage of post larvae (P14) . At post larvae 40 (P40) stage, Vibrio was detected together with Pseudomonas, Moraxella and Flavobacterium. After post larvae 60 (P60) and in adult stages the microflora of the alimentary tract were mostly composed of Vibrio. On the other hand the microflora of integument consisted of Pseudomonas, Flavobacterium, Moraxella and Vibrio, which were considered to be predominant in the environmental water, regardless of the sampling stages. Vibrio strains isolated from prawns as dominant bacteria showed the decomposing activities for macromolecules such as casein, starch and chitin.
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  • Characteristics of Vibrio sp. Isolated from the Intestine of Healthy Prawns and the Muscle of Diseased Prawns
    Taizo SAKATA, Nobuyuki TARUNO
    1987 Volume 35 Issue 3 Pages 153-160
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
    Ten strains of Vibrio spp. including representative strains isolated from the intestine of healthy prawns and the muscle of diseased prawns, and two reference strains were examined for 81 characters based on their morphology, biochemistry and physiology to be subjected to numerical taxonomic analysis. Intestinal isolates comprised two strains closely related but differrent to Vibrio fischeri and one strain identical to V. fluvialis II. Muscular isolates included a variety of species such as luminescent V. fischeri, V. fluvialis II, and those closely related to V. fischeri and V. anguillarum.
    Adult prawns were injected intramuscularly with different strains at different bacterial concentrations. Death occurred in the prawns injected with 0.1ml of cell suspensions above 106cfu/g of body weight regardless of Vibrio strains which were isolated from the muscles of dead or moribund prawns.
    These results suggested that cultured prawns were caused to die with bacterial cells which moved from the external surfaces such as the intestine, gill or integument and grew rapidly in the internal organs of prawns as the defense barrier of hosts might be injured.
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  • Yukio UETA
    1987 Volume 35 Issue 3 Pages 161-169
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
    Reproduction and growth of a penaeid prawn Trachypenaeus curvirostris (STIMPSON, 1860) in Kii Channel, Japan, was studied. Relationships between prawn size and the gonad somatic index showed that the smallest size for spawning females was 55 mm body length. Individuals in the early hatching group became large sized females and the spawning period was from mid May to July. Furthermore, those in the later hatching group were small and spawned from August to early October. Consequently, spawning season of the prawn was from mid May to early October. Seasonal change of the mean body length of each group by sex represented that females in the early hatching group and those in the later hatching group finally reached to 95mm and 80mm in body length, respectively, and males grew 60mm in both groups. The life span of this species was 12 to 19 months after hatching.
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  • Shiro ITO, Atsushi KOBAYAKAWA, Yusaku TANI
    1987 Volume 35 Issue 3 Pages 171-174
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
  • Katsuhisa YUKI
    1987 Volume 35 Issue 3 Pages 175-182
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
    An attempt to mass-produce the seeds of Japanese scallop, Chlamys farreri nipponensis, was made in Matoya Bay, Pacific coast of central Japan, using the adults transferred from Miyagi Prefecture, northern Japan. Before the spawning-inducement, mature adults were kept at 17-18°C for three to four days. In July-August 1986, the ripe eggs and sperm were spawned by transferring them to the ammoniac seawater at ca. 24°C. The males were readily induced, whereas the spawning females were confined to 45% among the individuals used. At 25-26°C, the spawned eggs (ca. 70μm in diameter) developed to D-shaped veliger (85-100μm in length, 70-80μm in height) about 20 hours after fertilization. The larvae, which fed on Pavlova lutheri, ended their planktonic life by the nineteenth day after fertilization and grew up to early spats. The maximum shell length of fully developed larvae was 190μm, and the minimum prodissoconch shell length of early spats was 160μm. These indicate that the shell size of the metamorphosing stage larvae is 160-190μm in length. The young spats were transferred from the tanks to the sea when they reached about 1 mm in the mean shell length and suspended at 5 m depth from the raft. After two months, they exceeded 10 mm in the mean shell height. The individuals representing 0.5% of the D-shaped larvae survived to this size.
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  • Katsumi HIRANO, Tsugio FUJIWARA
    1987 Volume 35 Issue 3 Pages 183-189
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
    Corbicula leana PRIME which hatched on May 1975 was cultured in the experimental pond (2m2) in Kobayashi City, Miyazaki pref. and its growth and life span were studied for about 8 years. The results obtained from the experiment are summarized below.
    1. On the growth ofC. leana, drastic changes were observed at about 13mm, 21mm and 25mm in shell length. Between the growth in the shell length and the day after the hatching out, a relationship which shows an algebraical function is found.
    2. The maximum shell length of C. leana cultured in the experimental pond is 33.3 mm, the maximum life span is 8 years and 2 months.
    3. Mean shell length of C. leana reached 15.6mm in the first year after hatching out from May 1975 to April 1976. The growth in shell length in the first year reached about half of the total growth in 8 years and 2 months.
    4. C. leana with shell length of 54mm was collected in a pond in Kobayashi City. Judging from the calculation on growth equations, the life span of this shell was estimated to be about 17years old or more. As its longevity is more than that of other species, a careful reassessment of the life span of all species is required.
    5. The growth of younger C. leana less than full 4 years old is especially under the influence of water temperature. More than 85% of the total growth in a year is shown during spawning season which is from April to October, and mean water temperature of month is more than 16°C. Less than 14% of the total growth in a year is shown during the rest season and mean water temperature is less than 16°C.
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  • Hideo YAMANOI, Teruyuki SUGIYAMA
    1987 Volume 35 Issue 3 Pages 191-195
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
  • Eijiro KAWAHARA, Riichi KUSUDA
    1987 Volume 35 Issue 3 Pages 197-199
    Published: December 01, 1987
    Released on J-STAGE: March 09, 2010
    JOURNAL FREE ACCESS
    A comparison of count of Streptococcus sp. by fluorescent antibody technique (FAT) and plate culture method was performed. Broth cultured Streptococcus sp. and diseased fish tissues, brain, kidney and spleen were used as counted samples.
    Numbers of Streptococcus sp. in these samples were, material, by FAT (cells/ml or g), by plate culture method (CFU/ml or g) : broth culture, 109, 107-108; brain, 108-109, 106-107; kidney, 107-108, 105-106; spleen, 106-107, 104-105 approximately. The FAT counted the bacteria about 100times as much as the culture method.
    The plate culture mothod can not count Streptococcus sp. correctly, because this bacterium show a chain or mass in broth culture or diseased fish tissue.
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