アレルギー
Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
10 巻, 7 号
選択された号の論文の7件中1~7を表示しています
  • 原稿種別: 目次
    1961 年 10 巻 7 号 p. 1-4
    発行日: 1961/07/30
    公開日: 2016/10/20
    ジャーナル フリー
  • 樋口 謙太郎, 井上 浩一, 岡 幸広, 松村 武正
    原稿種別: 本文
    1961 年 10 巻 7 号 p. 383-390
    発行日: 1961/07/30
    公開日: 2016/10/20
    ジャーナル フリー
  • 田辺 功
    原稿種別: 本文
    1961 年 10 巻 7 号 p. 391-399,412
    発行日: 1961/07/30
    公開日: 2016/10/20
    ジャーナル フリー
    Process of sensitization in rabbits with some organ antigens and effect of organ antibody upon the reactivility of the corresponding organ-cells were studied and obtained the following results: 1. The anamnestic reaction in animal sensitized with liver extracted antigen was induced by the same reaction as it sensitized with heterogeneous proteins or bacillus. 2. The anamnestic reaction in animals sensitzed with homologous or heterogeneous liver antigens was induced remarkably by corresponding antigens rather than adrenocortical extract. 3. In the living organ sensitized with homologous or heterogeneous liver extract, reactions of the reticuloendotherial systems were almost the same with those sensitized with heterogeneous proteins. 4. In the electron-microscopic findings of the liver cells sensitized with liver extract, change of endoplasmic reticulum was almost invariably observed, as was seen in the liver cells sensitized with heterogeneous protein. 5. The electrophoretic mobility of the liver cells sensitized with liver extract or heterogenous proteins increased remarkably in the Tyrode solution to which the correspondent antiserum was added beforehand. 6. Phase contrast microscopic findings of the liver cells sensitized with liver antibody were changed according to the increasing electrophoretic mobility. By these facts, the changes of reactivility in the liver cells on the sensitizing process with the liver extract or heterogenous proteins were proved.
  • 田辺 功
    原稿種別: 本文
    1961 年 10 巻 7 号 p. 400-407,412
    発行日: 1961/07/30
    公開日: 2016/10/20
    ジャーナル フリー
    An immunoserological study was carried out on anti-liver-autoantibody in vivo by means of electrophoresis and phase contrast microscopy of the liver-cells. 1. The anamestic reaction could be induced by the administration of ACE in significant number of cases in whom the anti-liver-autoantibody had been found in the circulating blood. 2. Active pathological changes were still noted in those cases of viral hepatitis or cirrhosis in whom the anti-liver-autoantibody had been found in the circulating blood and reappeared following the ACE injection. 3. It was suggested that some cases of chronic hepatitis and livercirrhosis showed a clinical finding which was considered to be a sensitized state with the liver-antigen, and in this cases the anti-liver-autoantibody was evidently thought to be an etiologic factor for these liver diseases. 4. When in vitro, the anti-liver-autoantibody acted on the liver cells of patients who had a positive titer of anti-liver-autoantibody in the blood, the liver cells manifested more promoted roughness of granular substance in protoplasm, and more increased electrophoretic mobilities than the negative cases of anti-liver-autoantibody titer in the circulating blood.
  • 高橋 良
    原稿種別: 本文
    1961 年 10 巻 7 号 p. 408-411,413
    発行日: 1961/07/30
    公開日: 2016/10/20
    ジャーナル フリー
    With the purpose to trace the change of circulating antibody titer in ascaris allergy, Kuroume of this department previously applied the tanned cell agglutination technique which was, initiated by Boyden, and reported that an observable change in circulating antibody titer had been recognized not only in experimental but also in human ascariasis. Zoltan Ovary devised PCA (passive cutaneous anaphylaxis) using guinea pig skin, and found it positive for egg albumin and polysaccharide of Diplococcus Pneumoniae. This is a test of interest for antibodies, but nevertheless have not be applied in the field of parasitology. The author applied this test for ascariasis, and demonstrated circulating ascaris PCA antibody in ascaris-infected and ascaris body fluid-sensitized guinea pigs and also in animals bred in farmer's house without any precautions. And the author investigated relation among results of this test, tanned cell hemagglutination and precipitation. As the ascaris body fluid is used as the antigen intravenously in this animal experiment, for intravenous injection, it is of utmost necessity that guinea pigs must be non-infected, and to survive intravenous injection of a considerably large dose of the ascaris body fluid. On this account, the author used animals which were bred by special breeding method devised by this department in which animals are kept free from ascaris infection. Sera to be tested, either original or saline dilution, were intracutaneously injected in the abdomen in a dose of 0.1 ml., and after 4-6 hours, a mixture of each 0.5 ml of 10% body fluid and 0.5% Evans blue was intravenously injected. Other 15 minutes later, the site of the serum injection was examined, and when the diameter of blue staining was over 5 mm, the reaction was evaluated as positive. Results of the experiment in which the antibody absorbed completely by adding the same volume of the ascaris body fluid or the serum from non-infected guinea pig was used, was always negative. It is therefore, assumed that this reaction might be specific to ascaris. When non-infected guinea pigs were sensitized by single injection of 1 ml/body fluid, level of PCA antibody titer was proved to become to the highest after 3 weeks. In the experiment in which 5,000 ascaris ova were orally given at one time to non-infected guinea pigs, the level of the antibody became highest after 2 months. PCA antibody titer did not always change in parallel with titers of the hemagglutinin and precipitin, and therefore the former is considered to be different from the latter. Experiment for thermostability revealed that PCA antibody was destroyed by heating at 67℃ for 30 minutes. And out of 53 guinea pigs, which were bred without any precautions, 24 (45%) were positive in this reaction. From the results, it was thought that naturally bred guinea pigs in Japan might be infected with ascaris in variable grade.
  • 原稿種別: 文献目録等
    1961 年 10 巻 7 号 p. 412-413
    発行日: 1961/07/30
    公開日: 2016/10/20
    ジャーナル フリー
  • 原稿種別: 付録等
    1961 年 10 巻 7 号 p. 414-428
    発行日: 1961/07/30
    公開日: 2016/10/20
    ジャーナル フリー
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