As an approach to the immunological studies of cancer, changes of cancer antigenicity were carried out mainly by means of immunochemical analysis like as gel diffusion technique and immunoelectrophoresis, which were specific antigen-antibody reaction. Thereafter the following conclusions were obtained. 1) The results of biochemical analysis of serum protein fractions by Tisselius or some other methods did not show to be any specific for cancer, but the immuno-chemical analysis can show pretty significant factors of the specific antigenicity. 2) In serum or ascites of transplantable animal cancer as Ehrlich ascites tumor in mice and Yoshida sarcoma in rats, specific antigen ingredients of cancer could be found, although not be proved in normal animals. So these factors were electrophoretically in α_1- and α_2- glubulin region. 3) In serum and ascites in cancer patients, electrophoretic specifity were shown in α-globulin region, especially in α_1-globulin. 4) Those specipitation pattern in α-globulin region showed a little difference by cases and antiserum used. 5) Degrees of antigenicity of cancer serum have some relation to progress of cancer invasion and primary genetic tissue, therefore general protein metabolism strongly infiuences it and results quantitative difference. 6) In every tissue protein, specific antigens as organ specificity as well as common antigens, were demonstrated. 7) The alteration of antigenic structure of cells due to malignisation shows that the antigenic structure becomes more simple because of the disappearance of some normal antigens from the cancer cells(loss), on the other hand it becomes more complex in connection with the appearing of some new antigens in cancer cells (gain). It seems that this changes differences by the case and the stadium, of cancer. 8) Cancer specific antigens identified by gel diffusion method are very weak and these specific factors are soluble in 1/3 (NH_4)_2SO_4 saturated solution at 4 C, pH 7.0, but not in 1/2 (NH_4)_2SO_4 saturated solution at same temperature and pH, and are electrophoretic to positive pole. 9) As far as stomach cancer is concerned, cancer specific antigens are common with each stomach cancer beyond the individual speciflcity.

View full abstract

1) Renal anaphylactic shock was induced by single injection of the anti-rabbit-thymus guinea pig serum. In one group of the male rabbits the antiserum was injected into the left renal artery, and in the other male or pregnant rabbits the injection was carried out similarly into the marginal ear vein, and these glomerular changes of the rabbits kidneys were investigated using electron and light microscopy. 2) In these glomerular changes no significant differences were observed between male rabbits and pregnant rabbits more over two groups. 3) By light microscopy, remarkable swelling degeneration and necrosis of the endothelial cells, epithelial cells, partial in mesangial cells of glomerular tuft and tubular epithels, and by electron microscopy various findings were observed, namely mitochondrial swelling or pyknosis in light and disorganization of cytoplasmic organelles in severe glomerular lesion, and usual nucleus formed irregular. 4) These features are similar nature to those in the examinations of the effect upon the liver or spleen by same antibody or anti-histone serum, and these glomerular changes like the human glomerulitis in autopsied cases of malignant tumor or receiving blood transfusion in large quantity.

View full abstract

Studies on skin tests with mold allergens, some serological studies and hyposensitization thrapy with mixed mold extracts were as follows; 1) Five mold extracts were used for skin tests. They were scratch type of powdered allegens made by Hollister-Stier laboratories (Aspergillus, Penicillium, Hormodendrum, Rhizopus and Alternaria). One hundred twelve asthmatic children were tested with the mold extracts, fifty six cases (50%) were positive. Control group of nonasthmatic children were only 10 per cent positive. 2) Mixed mold allergens mde by Kudansake hospital were used for intradermal tests. One hundred eight-three asthmatic children were tested with our mixed mold allergens. Fifty one cases (27.3%) were positive. Control group of nonasthmatic children (20 cases) were negative. 3) PTT with mold extracts made by Hollister-Stier laboratories have been done in 13 cases. Five of them (38.4%) was positive and mixed mold made by Kudansaka hospital have been done in 15 cases, 14 of them (93.3%) was positive. 4) BDB were all positive in 11 cases. 5) Two years follow up studies of hyposensitization thrapy with mixed mold extracts showed no asthmatic attacks in 10 cases (58.9%) in 17 cases, but symptomatic therapy showed in only 6 cases (15.0%) in 40 cases. The result of hyposesitization therapy group were excellent compared with symptomatic therapy group.

View full abstract

1) Penicillium, Aspergillus, Hormodendrum, Macrosporium, Alternaria, Monilia, Phizopus, Tolura, Streptomyces, Trichoderma, Fusarium, Saccharomyces were found. 2) Aspergillus, Hormodendrum, Penicillium were main molds according to my survey, and were found in all year round. 3) Total mold spore counts revealed an increase generally in hot season (July, June, August, September), and decrease in cold seaseon (Janually, Febrary, March). 4) Panicillium was increase in Summer and Fall, Hormodendrum in Summer, and Aspergillus in Summer and Fall. My study revealed a high incidence of positive skin tests, presence of atomospheric molds counts, positive PTT and BDB-reaction, and effective hyposensitization therapy with mixed mold extracts. I emphasized aerogenic molds are also one of the causative factors of bronchial asthma in childhood.

View full abstract