The hyposensitization treatment with house dust allergen followed "the Scratch-Method" were carried out to 131 cases of asthmatic children. The technique which was nominated here as "the Scratch-Method" had been devised in our clinic at first time, was executed as followed; the several scratchings in the length of a certain mm in I square centimeter, made mildly be bleeding enough, were performed on the extensor site of arm and there dropped and smeared the allergen extracts. And then gradually made prolong the dropping and smearing duration in schedule through which made attempt to increase of the dosis of allergen and at the same time made also prolong the interval of treatment with the tide of therapeutic process. The allergen extracts, used in this study, were consisted with that of the mixture in which mixed equally in volume of 10 sorts of house dust allergen extracts which were extracted with the CoCa solution and which had been usually utilized to the scratch test in our clinic. The subjects were observed the process for from 15 months to 32 months and the author have attempted to estimate to the effects of this therapeutic trial. The effects was found out in 80.9% followed the criteria which had been established by the meeting society of pediatric allergy, and in 85.0% were improved from the view of point tht of the Savidge, therefore, it is able to be admitted that the effects of this trial therapy had not significantly differed from that of the usual technique as the intradermal injection. Some observation made in this study about relationship of the effects to the term of treatment, sex, and appearance of the side effect was also noted. 1) The more improved, the longer treated in its term and the more frequent treated. 2)There was no significant difference of the effect in sex. 3) The side effects were noted as the occurrence of urticaria, turned severe eczema, and immediated or delayed reaction etc. And provocated mild asthmatic attacks were checked 45.8% in its occurrence. This study is at all the first trial one which little work has been done in any literatures, so that the author methinks about some merits and faults in this treatment and then from these results, emphasize that this attempt is one of the reliable and useful therapeutic method of hyposensitization.

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As an approach to the immunological studies of cancer, the author investigated the antigenicity of subcellular fractions of tumor cells by fluorescent antibody technic. Antisera to each subcellular fractions of Ehrlich ascites tumor cells and of AH 130 cells were made in rabbits, and besides, 3 fractions prepared from nuclear fraction of AH 130 were also used in immunisation. Using these antisera, tumor cell specificity was investigated by precipitation, complement fixation test, and fluorescent antibody staining. As the control organ, mouse liver for Ehrlich tumor cells, and rat liver for AH130 cells were used and the staining properties of antisera after absorption were examined. Following conclusions were obtained from above experiments. 1) Concerning Ehrlich ascits cells, relatively tumor specific antigenicity were found in nuclear and microsomal fractions. Specific fluorescent staining of tumor cells was obtained, after absorption of antisera by mouse liver, but staining was weak. 2) Concerning AH 130 cells, relatively specific tumor antigenicity by CFT was obtained in nuclear fraction, acid protein component, insoluble fraction, and microsomal fraction. By fluorescent antibody staining, the highest tumor cell specificity was found in microsomal fraction and the highest staining titers was found in anti-insoluble fraction. But by investigation of cross reaction to normal organs and tissues, these cross reactions to some tissues, esp. connective tissues were not completely excluded. 3) The same investigation was made in human gastric cancers, but clear cancer cell specific fluorescent stain was not obtained in common among difterent individual materials, except in one case, in which cancer cell were clearly stained by antiserum to difterent materials. 4) Antiserum prepared by normal human gastric mucosa, stains normal human gastric surface epithelium, but this staining was negative by anti-cancer sera. Loss of some antigenicity in cancer was suggestive.

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