アレルギー
Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
15 巻, 1 号
選択された号の論文の13件中1~13を表示しています
  • 原稿種別: 表紙
    1966 年 15 巻 1 号 p. Cover1-
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 表紙
    1966 年 15 巻 1 号 p. Cover2-
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 付録等
    1966 年 15 巻 1 号 p. App1-
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原田 稔, 竹内 三津男, 片桐 謙
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 1-7,62
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    For the evaluation of anti-allergic effect of drugs, it is necessary to work out quantitatively in some allergic reaction system in vivo. For this purpose, passive cutaneous anaphylaxis (PCA) is considered preferable. This is because it would be possible to determine the degree of reaction exactly in terms of the amount of dye infiltrated in the skin; although the extraction of the dye has been a very difficult problem. At the beginning of this investigation, therefore, we searched for a good method to extract the dye and obtained an easy and satisfactory one. Based on the establishment of this method, which is described below, the authors set out a series of experiments to evaluate the drug action against PCA. It is desirable to see whether cutaneous anaphylaxis corresponds in every respect to systemic anaphylaxis. Therefore, the action of drugs was also examined against passive systemic anaphylaxis (PSA). In this paper, as the first attempt, the effect of anti-histaminics was tested in guinea-pigs, as histamine is assumed to be the chief mediator in the anaphylactic reaction in guinea-pigs. Throughout the experiments in this report, bovine γ-globulin (BGG) and rabbit anti-BGG serum were used as antigen and antibody respectively. The results are summarized as follows: 1) The following precedure was proved to be satisfactory to extract the infiltrated dye. That is, the infiltrated dye, Evans blue, was easily and quantitatively extracted from the skin by stirring the chopped tissue in a homoblender with the mixture of acetone and aqueous solution of the detergent "Emal S" (Kao Soap Co.). After centrifugation, the dye was determined by measuring the optical density at 620mμ of the supernatant. 2) When administered intraperitoenally, all the three anti-histaminics (promethazin, triploridine and diphenhydramine) inhibited histamine injected intradermally very strongly. On the other hand, PCA was considerably inhibited by higher doses of promethazine (>5mg/kg) but was affected only a little by triploridine and diphenhydramine, when administeread 1 hour before the injection of antige. 3) Contrary to the intraperitoneal injection, intradermal administration of a very small amount of these drugs together with antiserum caused a conspicuous inhibition of PCA. This inhibition was obvious when antigen and the dye were given immediately after the intradermal injection of a drug mixed with antiserum. Accordingly, it may be considered that the liberation of histamine contributes something to the manifestation of PCA in guinea-pigs. 4) PSA were clearly inhibited by the intraperitoneal injection of promethazine or triploridine, though only slightly by diphenhydramine. Therefore, it may be assumed that histamine is the main mediator in PSA.
  • 光井 庄太郎, 佐藤 郁郎, 工藤 康之, 小山 陽三, 細川 久昭
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 8-12,63
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    In the resected lung of an asthmatic patient pathohistological examination and determination of histamine were performed. The fourty-two year old male, who had attacks of bronchial asthma since the age of thirty-six, received right upper lobectomy for pulmonary tuberculosis on May, 1964. Following observations were performed in the part of the resected lung, in which tuberculous lesion was not confirmed. Pathohistological findings: The mucous membrane of bronchi was somewhat full of folds and their lumen was narrowed. In more or less large bronchi cilia of epithelium were partly indistinct and cubical epithelia were scarcely found. The epithelia of smaller bronchi were generally cubic or flat. The basement membrane showed features of hypertrophy and hyalinization in some places and the submucosal layer was hypertrophic in general and homogeneous in part. The hypertrophy of muscle layer was striking. In the wall of bronchioli and also around them eosinophils infiltrated in groups with slight infiltration of such round cells as lymphocyte and plasmocyte. A small quantity of secretion, desquamated epithelia and eosinophils were found within lumina of bronchi. The lung was generally emphysematous, but in part atelectatic portions were seen. Alveolar walls were, as a whole, slightly hypertrophic and connective tissue was also somewhat proliferative. Infiltrations of eosinophils were seen in some places of alveolar septa. Elastic fibers were large and a lot of them were broken. Vascular walls were hypertrophic in general and phagocytes containing hemosiderin were seen diffusely or in groups in them. Histamine content: Histamine of the lung tissue was measured by McIntire's method. The histamine contents of healthy portions in resected lungs of three patients with pulmonary tuberculosis were measured as controls and the average value of them was 29.8γ/g. The histamine content of the resected lung in the present patient was 79.5γ/g. Thus it was suggested that the histamine content of lung tissue of asthmatic patient was much increased as compared with that of non-asthmatics.
  • 三島 隆
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 13-20,63-64
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    With the aim of examining the reactivity of rheumatoid factor with gamma globulin of the ten kinds of animal, namely, human, rabbit, equine, bovine, guinea pig, rat, dog, chicken, goat, and sheep, the following results were obtained from the "tannic acid treated hemagglutination test" and "hemagglutination inhibition test with gamma globulin", and also "the absorption test of rheumatoid factor with insoluble gamma globulin treated by bisdiazotized benzidine". 1) Rheumatoid factor both in sera of rheumatoid arthritis and in sera of liver cirrhosis somewhat reacted with 7S gamma globulin of various kinds of animal, and it was shown that there was a tendency for the intensity of its cross reactivity to differ according to the sera used. 2) The rheumatoid factor which reacted with equine and rabbit gamma globulin in bisdiazotized benzidine treated gamma globulin column chromatography, also reacted with human gamma globulin, but there did not exist such a rheumatoid factor that only reacted with equine or rabbit gamma globulin. 3) Generally speaking, in "the inhibiton test against tannic acid treated hemagglution test, the same kind of gamma globulin as that used as reactant, showed the most intense inhibition.
  • 岡田 長保
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 21-28,64
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Bovine serum protein component, which is functionally identical with a mouse isoantigenic γ-Globulin (complement component) controlled by a single gene, was isolated by using Hc^0/Hc^0 mouse serum as a "Reagent". The purified preparation showed one β-migrating protein on immunoelectrophoresis using rabbit anti-bovine serum, and one antigen-antibody precipitate line against the same antiserum on the agar gel double diffusion test. But no cross reaction with the antiserum of Hc^0/Hc^0 mouse anti-Hc^1/Hc^1 mouse serum was recognized. The purified β-globulin was quite stable in its hemolytic activity even after being heated at 56℃ for 60 min., and it resisted a rather low pH. (4.2), for one month. The β-globulin allowed completion of the lysis of sensitized sheep erythrocytes in the presence of Hc^0/Hc^0 mouse serum, even when the reaction medium contained 0.01 M EDTA (Tetra-sodium Ethyle-nediaminete traacetate). The molecular size of the β-globulin appeared to be in the 7S category according to the elution pattern from Sephadex G-200 column. The purified β-globulin preparation had approximately 22-fold activity with respect to original bovine serum, and the rate of hemolytic reaction during the first 300 sec. was a liner function of the β-globulin concentration in the presence of Hc^0/Hc^0 mouse serum. On the basis basis of the characteristics of the β-globulin, the relationship between allergy or immunity and this protein was discussed.
  • 牧野 荘平
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 29-36,64-65
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Employing a newly constructed device, the intrapulmonary deposition of inhaled aerosols was measured quantitatively. The bronchial sensitivity to acetylcholine (Ach) or histamine (Hist) in bronchial asthma was represented by the respiratory threshold to Ach or Hist (RT-Ach or RT-Hist), i.e., the minimal amount of intrapulmonally deposited Ach or Hist to cause the initial decrease of FEV 1.0 more than 10 percent. RT-Ach and RT-Hist in asthmatic patients were significantly low, comparing to those valves in normal subjects. Relatively high correlation was found between RT-Ach and RT-Hist. RT-Ach and RT-Hist showed a sinigificant negative correlation with %FVC/predicted VC, %FEV 1.0/predicted VC and FVC/predicted VC. RT-Ach showed significant correlation with the score representing asthmatic symptoms for one year prior to the test or since the onset of asthma, and with the duration of asthma, while there was no significant correlation between RT-Ach and the score representing asthmatic symptoms for 2 weeks prior to the test. RT-Hist showed almost the asme relation with asthmatic symptoms and the duration of asthma as RT-Ach did, though the correlation with asthmatic symptoms for one year prior to the test was statistically not significant. Investigation of asthmatic symptoms for relatively long period prior to the tests suggested that asthmatic patients with increased respiratory sensitivity to the chemical mediators tended to have more frequent and severe asthmatic attacks.
  • 山県 英彦, 小川 弥栄, 仁士 賢一, 安保 純郎
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 37-47,65
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    Ogawa et. al have previously reported that the cavitation by infection of staphylococci was promoted by antigen-antibody reaction and that intravenous inoculation with heat-killed staphylococci suspended in saline induced protective effect against the cavitation. Accoring to these findings, it was attented to prevent the formation of cavities and/or necrosis in the lungs of guinea pigs and rabbits. To produce the cavities, either sensitized or nonsensitized animals with heat-killed staphylococci were injected into the lungs with same killed bacilli. To prevent the formation of cavities, procedures were used as follows. Animals (1) were subcutaneously injected daily with various doses of cortisone acetate, "Strong Neo-Minophagen C" and "Glycyron" and (2) were intravenously injected three times a week with heat-killed staphylococci suspenedd in saline. Two or four weeks after pulminary injection, animals were sacrificed for post-mortem examinations. Serum agglutination tests were performed on all animals once a week. The titers of animals treated with anti-allergics were lower than that of the non-treated animals. On the contrary, when the animals were injected intravenously with the bacillary cells, their agglutination titers were higher than that of the non-treated animals. Productive and proliferative changes were pronounced in the lungs of treated animals, while intensively exudative as well as necrotic and cavernous changes were found in those of the non-treated animals. From these findings, the authors suggested that the repeated injections of anti-allergics or antigen appear to have altered the usual process of development of necrotic and cavernous lesion.
  • 里見 智正
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 48-54,66
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    1) Cl. Welchii, the normal resident micro-organisms of the intestinal tract, produces several exotoxins, such as α, θ, κ and μ, and one of the, θ-toxin, is the hemolysin. The specificity of ASLO in some diseases has been elucidated, however, few studies have been made on the antihemolytic action of Closto-lysin θ which colsely resembed to Streptolysin O. Therefore, anticlostolysin θ (ACLθ) in various diseases was measured. ACLθ was measured by the non-reduction method which used the supernatant of Cl. Welchii PB6K cultured in the brain heart infusion broth, and also by the reduction method using the cysteine. In the former method, a high of ACLθ was seen in the groups of nephrosis, liver cirrhosis and hepatitis, and in those with high cholesterol. In the latter, it was high in the group of acute heaptitis, and the correlation to the serum bilirubin was found. Todd reported that cholesterol inhibited clostolysin, but the abnormal high value of ACLθ in nephrosis could not been explained only by this fact. Such correlation was not observed in the reduction method. In the human serum, the anti-hemolytic activity was high in the pseudoglobulin fraction, while in the sensitized rabbit it was found in the euglobulin fraction, and it was high in healthy subjects who treated Cl. Welchii, so at least a part of it was thought to be antibody. Anticlostolysin titer was measured in various diseases and its value was found to be high in nephrosis and hepatic disorders.
  • 里見 智正
    原稿種別: 本文
    1966 年 15 巻 1 号 p. 55-61,66
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
    2) Renal lesions caused by the sensitization with Cl. Welchii toxin, the supernatant of Cl. Welchii culture ranging from 5×0.5 ml to 3.0 ml was injected intravenously, gradually increasing thd dose, 50 times into the rabbit. The autopsy was carried out 5 days after injection. ACLθ titers and γ-globulin fraction of serum protein increased markedly after sensitization. Histologically, swelling and adhesion of glomeruli, exudation into Bowman's capsule, partial atrophy and fibrinoid degeneration, irregular hyper-trophy of basement membrane, and tubular degeneration were observed, but albumin in urine was negative. Using the supernatant of Cl. Welchii culture and the suspension of the bacillus, the long-term sensitization of guinea pigs and rabbits was made. In the guinea pigs ACLθ markedly increased after sensitization. Histologically, swelling of glomerular capillary loops, diffusecongestion of glomeruli, and partly the irregular fibrinoid degeneration of glomerular loops and the polynuclear leukocytic infiltration, similar to the acute period of nephritis, were observed. In the rabbits, congestion of glomeruli, irregular hyper-trophy of capillary interstitium, atrophic degeneration of the tubular epithelium and the infiltration of round cells into the interstitium were found. To observe the influence of Schwartzman phenomen by Cl. Welchii on the allergic reaction of the kidney, rabbits were sensitized by egg albumin and then mixed solution of egg albumin and the supernatant of Cl. Welchii culture was injected. Hiatological changes mentioned above became more markedly this procedure. Lesions similar to glomurular nephrosis and nephrosis were produced in expermental animals by the sensitization with Cl. Welchii toxins.
  • 原稿種別: 文献目録等
    1966 年 15 巻 1 号 p. 62-66
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
  • 原稿種別: 文献目録等
    1966 年 15 巻 1 号 p. Misc1-
    発行日: 1966/01/30
    公開日: 2017/02/10
    ジャーナル フリー
feedback
Top