The author has already reported that the ciliary movement of the respiratory epithelium from the sensitized guinea pigs was specifically suppressed by the antigen in vitro. In order to know the ciliary activity during asthmatic attack, the author presented in this paper some experimental results in the guinea pig. Used antigen was"Maiko"which is the offending allergen of Konjac bronchial asthma in human beings, and is a dust from Amorphophalus Konjac K. Koch. The fragments of the ciliated epithelium of the guinea pig obtained by scratching the tracheal mucosa with the strate curett after the established inhalation. The frequency of ciliary movement was estimated by cinematographic recording under the phasedifference microscope. The frequency of ciliary movement was markedly decreased when the sensitized guinea pigs were exposed to"Maiko", whereas that of non-sensitized one was not influenced with"Maiko"inhalation. On the other hand, in acetylcholine-induced asthma, the frequency of ciliary movement was moderately decreased, but only slight decrease was observed in histamine-induced asthma. These results suggest that ciliary activity plays and important role in development of bronchial asthma, however, the allergic asthma seems to be different from drug-induced asthma.

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In 1965, an antigenic substance was extracted from patient's urine suffering for allergic diseases (Soeda). This substance was named MS-antigen, and it is now obtained in an amorphous and powdery form. It is almost non-toxic and can induce production of precipitin when inoculated into man and animals. It was experimentally shown that serum titers of precipitin are rapidly enhanced by its repeated injection and maintained for fairly a long period after its administration is discontinued, and that allergic manifestations are almost completely suppressed as far as serum titers of precipitin are maintained significantly high. We have treated patients with various allergic diseases with MS-antigen and achieved fairly excellent results as follows: 1. Total 62 patients with allergic rhinitis were treated and excellent therapeutic results were achieved in 46 cases (74.2%) of the cases and 11 cases (17.7%) good response. 2. Total 38 patients with skin lesions were treated and excellent therapeutic results were achieved in 32 cases (84%) and 3 cases (7.9%) good results. 3. Total 30 patients with asthma bronchial were treated with MS-antigen, and now 20 patients are completely well, while 7 cases have been much improved. The remaining 3 cases did not respond to this therapy, except for temporal improvement. 4. 2 patients with rheumatoid arthritis (acute exacerbation) were treated and excellent therapeutic results and no recurrence for about 14 months. There appears to exist at least two types in MS-antigen, A and B, and accordingly patients with allergic diseases may be divided into A and B groups depending upon their serum response to either type of MS-antigen. The serum titers of precipitin should be maintained high to inhibit recurrence of allergic diseases.

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Na-Cu-Chlorophyllin (Cu-C) has been reported to be a complement inhibitor by Sindo and coworkers. Nishioka has found that the reactivity of C′5 was blocked by Cu-C, and Nelson has revealed that the interaction of C′1 and antigen-antibody complex was also blocked by Cu-C. Pillemer and coworkers have demonstrated that the inactivation of human C′2 and C′4 by plasmin is an indirect reaction mediated by a factor closely resembling C′1. Kondo and coworkers have found that the PCA reaction is inhibited by plasmin inhibitors, and that enhancement of vascular permeability by intradermal injection of C′1 esterase is also suppressed by plasmin inhibitors. Thus, it has been suggested that some interrelationship between the plasmin and the antigen-antibody-complement system, especially C′1, may exist. The results described herein were obtained from the experiments undertaken on the assumption that Cu-C, the complement inhibitor, may inhibit fibrinolytic activities of the plasmin. The inhibiting ability to the plasmin was examined by means of the standard fibrin plates. It was found that a small amount of Cu-C (5-20γ/ml) inhibit partially fibrinolytic activities of the plasmin.

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Previously, it was demonstrated that food allergy played an important role in the etiology of Orthostatic Albuminuria (OA) associated with such food allergy symptoms or diseases as migrain, eczema, gastric ulcer or duodenal ulcer, chronic gastritis, dizziness, lassitude, motion sickness and abdominal pain. Now, the following experiments were undertaken in an effort to demonstrate food allergy in the pathogenesis of OA found in healthy school children. I) OA found in healthy school children: OF 46 healthy children in one class aged 11 to 12, 18 were diagnosed as having OA upon the erect-lordotic posture; quantity of protein demonstrated by Albustix in urine was 300 mg in 5 cases; 100 mg in 6 cases and 30 mg in 7 cases. In addition, symptoms such as abdominal discomfort, headache, dizziness, eczema and papular urticaria were found to a slight degree in some of these children with OA. II) Effect of elimination of suspect food on the disappearance of OA: Upon the complete withdrawal of both milk and eggs, 11 cases showed the disappearance of albuminuria, 4 cases showed traces of albuminuria and the remaining 3 cases showed still the same amount of albuminuria as before elimination. In the latter 3 case, then, soybean and its products were completely eliminated, resulting in the disappearance of albuminuria on the erect-lordotic posture. III) Provocative test by giving the suspect food: Then, a provocative test was carried out by examining whether OA was produced or not when milk, egg or soybean was testatively given. As the result, following food allergens were confirmed to be capable of eliciting OA on the provocative test; milk for 6 cases and soybean for 3 cases, but in one case, no OA was produced even though milk, egg or soybean was given. In these cases the quantity of protein in the urine before elimination was compared with that on the provocative test; 8 cases showed the same amount of protein both times, 8 cases showed far smaller amount of protein on the provocative test than before elimination, and the remaining one showed a slighty larger amount of protein in the former than in the latter. On the provocative test with food allergen in these children, papular urticaria developed in 3 cases, nausea, headache and lassitude appeared in 4 cases within several days, in addition, cold sweating, nausea, malaise and pollor occurred in 8 cases shortly after erect-lordotic posture was taken. IV) Results of allergic skin tests: As to the skin test with lactalbumin and soybean extract, all cases were scratch test negative with the both allergens, while 3 cases were intracutaneous test positive with lactalbumin and 6 cases were positive with soybean extract. Thus, in all of 18 OA cases found in healthy school children food allergens were demmonstrated by elimination and trial ingestion diets. The result would give further support to the hypothesis that food allergy might play an important role in the etiology of OA.

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