Japanese Journal of Allergology
Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
Volume 17, Issue 12
Displaying 1-10 of 10 articles from this issue
  • Article type: Cover
    1968 Volume 17 Issue 12 Pages Cover7-
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
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  • Article type: Cover
    1968 Volume 17 Issue 12 Pages Cover8-
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    Download PDF (34K)
  • Article type: Appendix
    1968 Volume 17 Issue 12 Pages App10-
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
  • Masahiro Okuno
    Article type: Article
    1968 Volume 17 Issue 12 Pages 911-928,973
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    Forty-one rabbits were subjected to the direct antiglobulin technique with specific antiglobulin reagents. Twenty-six rabbits initially proved to be IgG globulin class alone; 12 rabbits initially demonstrated IgG plus IgM globulin class. Very few rabbits started with IgM alone. In most instances, IgG globulin calss autoantibody was constantly observed, while IgM was not always demonstrated during 60 day's observation. In the indirect antiglobulin technique, IgG was initially demonstrated in 10 out of 13 rabbits. IgG was constantly demonstrated on either the direct or the indirect antiglobulin technique, while IgM was not always demonstrated on either antiglobulin technique. Many rabbits initially proved to be IgG type later added by IgM type. IgG globulin class autoantibody seemed to be the main component in anemic rabbits. The antiglobulin technique with specific anti-rabbit c' serum proved to be positive in many instances. But such positive result was not constantly demonstrated in the present study. Therefore it is led to consider that complement component may be very labile. It is considered that incomplete autoantibody against erythrocytes is different from complete antibody against egg-albumin on the basis of the present data that 1) complete antibody against egg-albumin is detected prior to incomplete autoantibody against erythrocytes, 2) initially incomplete autoantibody against erythrocytes proved to be IgG globulin class, but complete antibody against egg-albumin proved to be IgM globulin, and 3) there is no difference in the titer of indirect antiglobulin test of anemic rabbits following absorption with egg-albumin.
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  • Akira Ohkita
    Article type: Article
    1968 Volume 17 Issue 12 Pages 929-945,973-97
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    A report was made of the effect of prednisolone on hemolytic anemia produced in rabbits following repeated intravenous injection of egg-albumin. In a group of 15 anemic rabbits subjected to a 3-day administration of prednisolone, 11 rabbits showed a prompt but temporaty improvement of anemia, 5 rabbits became temporarily direct and indirect antiglobulin test negative. Following administration of prednisolone at a gradually decreased daily dosage from 3 mg to 0.5 mg per kg of body weight for 30 days, 12 in a group of 18 anemic rabbits had their anemia markedly improved and their Cr^<51>-labeled red cells longer survived, 8 of the 12 rabbits became direct and indirect antiglobulin test negative, the absence of the disappearance of complete antibody against egg-albumin. IgM and c' component of incomplete antibody against erythrocytes disappeared earlier than IgG component following administration of prednisolone. In nonresponsive rabbits, the disappearance of IgG and IgM was temporary. The development of hemolytic anemia in rabbits following administration of an eluate from incomplete antibody coated red cells was inhibited in 3 groups, each group consisting of 4 rabbits and 2 or 1 control, following treatment with prednisolone. The present experimental results revealed that in many instances direct antiglobulin test proved to be negative although indirect antiglobulin test was positive. It seems that administration of prednisolone prevented the injected incomplete antibody from being attached to the red cell in these rabbits without mechanism of incomplete antibody formation. It is concluded that the main effect of prednisolone on autoimmune hemolytic anemia is likely to consist of suppression of an incomplete autoantibody formation and prevention of incompleet autoantibody from being attached to the red cell. It is to be noted that mechanism of prevention of such attachment can not be ignored as a responsible factor of prednisolone for a prompt response in autoimmune hemolytic anemia.
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  • Tokichi Yumoto
    Article type: Article
    1968 Volume 17 Issue 12 Pages 946-957,974
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    Histological study was carried out on tisssue sections of 22 kidneys of New Zealand Black (NZB/Bl) strain mice which "spontaneous" lymphoid leukemia and autoimmune hemolytic anemia combined and also of 4 kidneys of normal control mice, aged one to three month-old. Renal lesions have been observed in section of kidneys of 17 out 22 adult mice (77%). No abnormal changes were fo und in all four normal control mice. Renal lesions consist of eosinophilic membranous thickening of glomerular capillary loops due to deposition of fibrin with PAS-positive material. The earliest histological changes was fibrinoid degeneration or hyaline thickening of capillary walls and adjacent intercapillary region of the glomeruli. Extention of this process, with dilatation of the hyaline degeneration produced narrowing of capillary lumens. In the later stages hyaline lobulation and conglomeration of the glomerular tuft soccurred. Reaction of Bowman's capsule is a mild in compare with glomerular changes. Tubular changes were not obvious until the glomerular damage is well in evidence. Tubular changes usually follows the glomerular changes. However there arecould be classified into three stages; earliest, advanced, later, by histological findings of combined glomerular and tubular changes. The "necrotising" arteritis developing in one case of later stage was seen. Perivascular tumor cell infiltrates were seen in 9 out 22 cases (40%). Tumor cell infiltrates presented along the course of the vessels as they extend towards the cortical region from hilar region. Renal lesions of the NZB mice are an exprimental model in the mouse for autoimmune disease, but it was unrelated to the infiltration by tumor cells. Glomerular changes are similar to those seen in human lupus nephropathy.
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  • Takeshi Miyazaki
    Article type: Article
    1968 Volume 17 Issue 12 Pages 958-963,975
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    In order to study cytotoxic effects of anti-liver antibody, an experiment was performed in vitro to detect fixation of the anti-liver-antibody to the liver cell membrane using antiserum obtained from the rabbit immunized with rat liver supernatant, with the following conclusions: 1. Tanned sheep red cells coated with liver antigen were added to the separated rat liver cells which had been incubated at 37°C for 24 hours and then previously sensitized by the antiserum. The red cells were observed to fix on the liver cell membrane. 2. Polystyren latex particles coated with the rat liver antigen were added to the separated rat liver cells after sensitization by the antiserum. The latex particles were fixed on the liver cell mombrane. From the experiment, it was proved that the antiliver-antibody fixes on the liver cell membrane. 3. An experimental method for detecting immunologically competent cells was deviced to study thier cytotoxic effect upon liver cells. Polystyrene latex particles coated with human liver antigen were mixed with the lymphoid cells obtained from the popliteal lymphnodes of the rat immunized with the antigen, and lymphocyte in the peripheral blood of the patient with chronic hapatic disease. The latex particles were observed to adhere to some of the lymphoid cells.
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  • Takeshi Miyazaki
    Article type: Article
    1968 Volume 17 Issue 12 Pages 964-972,975
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    Experiments were performed in vitro to study cytotoxic effects of anti liver antibody upon the rat liver cells suspended in Hanks' sol, to which anti rat liver rabbit serum and complement were added, by measuring released enzyme activity and endogenous respiration, and by counting 0.5% trypan blue stained liver cells. The results were as follows: 1. The liver cells, to which anti whole cytoplasm serum and complement were added, showed a high rate of 0.5% trypan blue stained cells and high activity of released enzyme, however, no distinct influence on the endogenous respiration was observed. 2. Antiserum to the mitochondrial fraction, with or without complement, did not revealed any distinct effect upon liver cells. 3. There was a difference in quality between anti whole cytoplasm serum and anti mitochondria serum in respect of the influence on the rat liver cells in vitro.
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  • Article type: Bibliography
    1968 Volume 17 Issue 12 Pages 973-975
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
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  • Article type: Index
    1968 Volume 17 Issue 12 Pages 976-980
    Published: December 30, 1968
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
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