The enzyme-labeled antibody technique reported by Nakane et al is an excellent immunohistochemical method because of availability of permanent specimen for light microscopy and of possibility of electron microscopic observation. We used FNPS (according to Nakane) or glutaraldehyde (according to Avrameas) as coupling reagents. The latter was found better than the former concerning the coupling rate of peroxidase with antibody and the titer of conjugated antibody. Peroxidase-labeled anti-human gamma-globulin prepared with glutaraldehyde method was applied to kidney biopsy specimen from 8 cases of diffuse glomerulonephritis in various stages and systemic lupus erythematosus and compared with the results obtained by the immunofluorescent technique. The immunoenzyme method seemed better regarding the precise localization of antigenic sites and semiquantitative distribution of antigens, but it appeard to be inferior in expression of granular or linear patterns which are well defined by the immunofluorescence. The conjugation procedure in the immunoenzyme technique is not so complicated and the conjugates are relatively stable at -20℃. Another advantage is that specimen for light microscopy are preservable. The immunoenzyme technique should be increasingly applied to the immunohistochemical analysis of kidney diseases.

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Pancreatin is a most ordinary digestive widely used in the form of powder or tablets with compounds. In this report is stated a case with occupational allergose, who was recently treated and observed in our Allergy Centre, and considered to be caused by pancrceatin. S.M., a chief pharmaceutist of a certain hospital, 46 years of age, took employment as pharmaceutist in 1949. He visited our clinic with the complaint of occurance of rhinorrhoea, sneezing or dyspnoea after the work of preparation of pancreatin drugs, having the experiences of asthmatic attacks over three times since 1955. The patient stated that these symptoms were not revealed while he was keeping out of his work on vacation or business trip. The remarkable peripheral leucocytic eosinophilia was not noticed, but eosinophilia in nasal smear was observed. In the intracutaneous test with inhalatory routine allergen extracts, no positive reaction was proved. And when the scratch-patch cutaneous test, nasal test, inhalative provocation test and Prausnitz-Kustner's passive transfer test with self-prepared pancreatin solution, were carried out positive reactions were assured. In the intracutaneous test with pancreatin solution was developed a conspicuous positive reaction, following severe asthmatic attacks, conjunctival congestion and urticaria eruption with remarkable itch. Judging from the above-mentioned clinical history and the results of allergic investigations, this case is considered to be the subject with occupational allergose caused by pancreatin. The author now points out the possibility that there exist the occupational allergose of many kinds among the pharmaceutists or drug manufacturers.

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The skin window technique according to Rebuck was use as a mean of study on mechanism of local eosinophilia in allergy. The following results were obtained. 1. Histamine, bradykinin, compound 48/80, a proteolytic enzyme, pronase and sialic acid were not eosinophilotactic. 2. House dust allergen extract produced a slight eosinophilia in all of the cases with house dust allergy examined. 3. Anti-human γG rabbit serum, human γG-anti-human γG complex, aggregated human γG, aggregated anti-γG serum and aggregated γG-anti-γG complex were not successful in eosinophilic response. The negative results were also obtained by application of anti-human γA rabbit serum, human γA-anti human γA complex, aggregated γA, aggregated anti-γA and aggregated γA-anti-γA complex. 4. Administration of anti-human γE rabbit serum showed a marked eosinophilic reaction. All of the cases examined revealed eosinophilic response when the undiluted antiserum was used, and positive reaction was produced in approximately 50 per cent of the cases examined with 1 : 10 dilution. There was no difference in frequency of positive response among extrinsic, intrinsic allergy and normal non-allergic individual. As higher as the concentration of the antiserum the intensity of local eosinophilia increased. An accumulation of eosinophils appeared between 1 and 3 hours after administration. The intensity of eosinophilic response in skin window was neither influenced by administration of antihistamine nor correlated with the intensity of skin reaction with anti-human γE rabbit serum.

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Recently allergic respiratory diseases are seemed to be increasing. The study of allergic bronchitis being with close relationship to bronchial asthma is indispensable to understanding pre-clinical stage of bronchial asthma. In this report 117 cases of allergic bronchitis who were diagnosed by diagnostic criteria presented by Dr.Seiji Kubo in 1969 were studied. 1) The incidence of this disease were one fifth of bronchial asthma. 2) Male is dominant in number as well as bronchial asthma. 3) These cases were divided into three types;27 cases belonged to type I, 55 cases to type II, 35 cases to type III. 4) The incidence of childhood asthma was the greatest during 6 to 7 years of age, but this disease was the greatest during 4 to 5 years of age in the type I, 1 to 2 years of age in the type II, and 5 to 6 years of age in the type III. 5) The major allergy of these cases and there family history were founded 83.7%. It is meaning that a atopic factor is of great significance in this disease. 6) The type II was more sensitive than other types on the bronchial response to methacholine. 7) The percentage of eosinophilia in nasar smea and sputum of this disease were 64.2% and 82.7%. 8) The percentage of the positive skin reaction with house dust were 19.4%, with ragweed 12.3%, with aspergillus 14.9%, with penicillium 18.4%, with alternaria 23.7%, with candida 37.7% and with cladosporium 20.2%. The rate of positive skin reaction with molds was higher than with house dust and ragweed. 9) The author treated these cases in 40.6% with specific hyposensitisation therapy, in 44.8% with non-spesific hyposensitisation therapy, and in 14.6% with symptomatic therapy. The effect of them proved good in 89.7% of cases who were treated spesific hyposensitisation therapy, but the effect of symptomatic therapy proved good only in 28.5%. 10) The effect (94.9%) of the hyposensitisation with molds was better than the effect (83.3%) of the hyposensitisation with house dust. Therefore the spesific hyposensitisation with mold is indispensable in therapy of the allergic bronchitis. 11) In the studies of skin test examined in the natural group of children, a positive skin reaction to candida was higher than to alternaria and penicillium. 12) Candida were cultured more highly from pharynx of cases with positive skin reaction to candida than from one of cases with negative skin reaction to candida.

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The activities of various antibodies on bronchial asthma were not yet throughly clarified. An attempt was made to investigate the roles of antibodies in guinea pig experimental asthma. In this experiment, in vivo and in vitro passive sensitization using the antisera or the immunoglobulins from sera of the sensitized guinea pigs was performed. To detect the activities of the antibodies, in vitro experiments including observation of ciliary movement of trachea and Schultz-Dale technique using intestine and tracheal spiral were used to simplify the experimental Systems. The following results were obtained. 1) The antibody capable to elicit the contraction of intestine and trachea were contained in a fraction of sera, Which were able to be transferred. 2) Heating at 56℃ for 4 hours or treating with 0.1 Mol.mercaptoethanol could not destroy the antibody activity, at least, under the terms of this experiment. 3) This antibody activity was found in a fraction which corresponded to Ovary's 7 Srl. 4) Suppressions of ciliary activities of the tracheal epithelial cells were also observed with same antigen-antibody system in the contractions of intestines and the tracheal spirals.

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The frequency distribution curves of average diameters of wheal and erythema were statistically analysed, which were obtained by the intracutaneous tests with the extract of house dust in 1526 inhabitants in rural and suburban districts as well as 265 asthmatics in the hospital. The frequency curves showed the bimodal character and were separated into two groups of non-specific and specific reaction by the methods of moments and sequential approximation. Then, the discriminative diameter between the positive and negative reaction was estimated to be 9 mm for the wheal, and 20 mm for the erythema. According to these criteria, the value of probability to make an error of the first kind (α) or of the second kind (β) is calculated to be 10% or less.

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