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Volume 23, Issue 6
Displaying 1-10 of 10 articles from this issue
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Article type: Cover
1974 Volume 23 Issue 6 Pages Cover33-
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (46K) -
Article type: Cover
1974 Volume 23 Issue 6 Pages Cover34-
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (46K) -
Article type: Bibliography
1974 Volume 23 Issue 6 Pages Misc7-
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (184K) -
Tadao IseArticle type: Article
1974 Volume 23 Issue 6 Pages 397-405,439
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSAdenyl cyclase activity of peripheral leukocyte from monkey and human being was determined by measuring the conversion ratio from ^3H-ATP to ^3H-cyclic-3', 5'-AMP. Reaction mixture was 1 ml of Tris-HCl buffer solution (4×10^<-2>M, PH 7.3), MgSO_4(3.3×10^<-3>M), theophylline (6.7×10^<-3>M), NaF(10^<-2>) or metaproterenol (10^<-3>-10^<-5>M), ^3H-ATP(10μCi, 10^<-3>M) and leukocyte suspension. Incubation was carried for 15-30 minutes at 30℃ with shaking. Before the completion of the reacion, 0.5mM of ^<14>C-8-cyclic-3', 5'-AMP (10mμCi) and 0.5 μmole of 5'-AMP were added as markers. On Dowex 1×4 (formate form) column chromatography, the cyclic-AMP fraction which contained the converted ^3H-radioactivity from ^3H-ATP and coincided with ^<14>C-radioactivity and optical density of marker, were completely separated from AMP and ^3H-ATP. It was possible by this method to recover ^3H-ATP sufficiently, which led to accurate calculation of the conversion ratio. The adenyl cyclase activity in monkey and human being leukocyte was proportionally increased as function of time till 30 minutes and as function of protein between 0.25 and 1.5mg. The elevated adenyl cyclase activity of leukocyte from monkeys(Macaca fuscate and Macaca fascicularis) receiving oral metaproterenol was shown with the decreased count of eosinophilic leukocyte. The reduced response to adrenaline in the monkey (Macaca fascicularis) receiving oral propranorol was ascertained by the no change of pulse rate and suger, lactate and eosinophilid leukocyte in blood. In the experimentel beta-adrenergic blockade of monkey, the leukocyte adenyl cyclase activity was lowered below the level before receiving propranorol.View full abstractDownload PDF (1116K) -
Tadao IseArticle type: Article
1974 Volume 23 Issue 6 Pages 406-416,439-44
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSAdenyl cyclase activity of peripheral leukocyte from asthmatic patients, rhinitis allergica and control subjects were estimated by the method presenting the previous report. The NaF stimulated adenyl cyclase activity of leukocyte from asthmatic patients was lower at attack than at no attack, and in the untreated group with bronchodilator than in the treated group with metaproterenol, irrespective of the type of disease. The metaproterenol stimulated leukocyte adenyl cyclase activity from asthmatic patients could not show so clear lowering of the activity at attack as observed in the experiment using NaF, nor the clear increase of the activity by administration of bronchodilator. As a whole, negative correlation was seen between the value of NaF stimulated adenyl cyclase activity of leukocyte from asthmatic patients and that of metaproterenol stimulated activity of the same leukocytes. Observing the asthmatic patients for a long term, NaF stimulated leukocyte adenyl cyclase activity at attack was significantly lowered more than natural change and in a little while after the attack the activity was recovered to the level before the attacks in most of the cases. At attack adenyl cyclase activity of asthmatic patients was lowered and the percentage of lymphocyte was decreased, and there was a negative correlation. This showed a striking contrast to the positive correlation between the activity and the increased percentage of neutrophil at attack. The changes of leukocyte adenyl cyclase activity in the various state of the asthmatic patients were discussed in relation to the beta-adrenergic blockade theory.View full abstractDownload PDF (1276K) -
Takao Shida, Kazuo Furuya, Hiroshi Ishiyama, Yoshinori Wagatsuma, Take ...Article type: Article
1974 Volume 23 Issue 6 Pages 417-422,440
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSTwo highly allergenic factions apparently of 35000 molecular weight have been isolated from the wormwood pollens by chromatography on DEAE-sephadex and on Sephadex. A third preparation of about 12000 molecular weight was also active though restricted in certain individuals. Other than the three allergens, various minor allergens were found in fractions on a starch gel, which was cut into 16 sections after electrophoresis. There were 4 groups among untreated individuals according to the mode of reaction to the starch gel preparations. Treated patients, on the other hand, showed mostly the same trend reacting predominantly to one fraction named as Mo.6, which could not yet by isolated by chromatographic method. A wormwood-sensitive patient was tested by the starch gel fractions successively in the course of desenditization. Except for No.6 to 8, his reactions to the fractions were getting weak. It is presumed that blocking antibodies to each active fraction could not be equally developed in the course of specific treatment.View full abstractDownload PDF (790K) -
Yosuke TanakaArticle type: Article
1974 Volume 23 Issue 6 Pages 423-429,440-44
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSCapillary Permeability in the skin of guinea pigs and rats induced by histamine, histamine liberator (compound 48/80) or human IgE・rabbit anti-human IgE complexes were studied and also the inhibitory effects of drugs against the capillary permeability were also investigated. Caplillary permeability was determined by the dye exudation technique and the results obtained were as follows: 1. Capillary permeability induced by the intracutaneous injection of histamine or histamine liberator (compound 48/80) was quantitatively determined by measuring the blueing dye diameter and the effective blueing dosis of histamine in the skin of guinea pig was 0.005μg and 0.009μg in the skin of rat. However, the effective blueing dosis of compound 48/80 in the skin of the guinea pig was 0.1μg and 0.07μg in the skin of the rat. 2. The intensity and the persistence of capillary permeability was also investigated. The strongest intensity of capillary permeability by the intracutaneous injection of histamine or compound 48/80 was observed a few minuted thereafter. 3. Capillary permeability was also observed in the skin of rats by intracutaneous injection of human IgE・rabbit anti-human IgE complexes consisting of 1 volume of 500 unit/ml human IgE and the same volume of 20 folds diluted rabbit anti-human IgE serum. 4. In the rats pretreated with pyribenzamine(10mg/kg), isoproterenol(10-50mg/kg), theophyllin(10-50mg/kg), prostaglandin E_1(100-500μg/kg) and D.S.C.G. (10-50mg/kg), capillary permeability by the intracutaneous injection of histamene, histamine liberator or IgE・anti-IgE complexes was inhibited.View full abstractDownload PDF (968K) -
Uki YamashitaArticle type: Article
1974 Volume 23 Issue 6 Pages 430-438,441
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSAntibody production against a haptenic group(para-azobenaoate:PAB)was elicited by the immunization with the hapten-isologous carrier conjugate(PAB-MγG)in mice and the above fact suggests that both T and B cells specific for the same haptenic group may develop by the use of non-immunogenic carrier (mouse immunoglobulin G:MγG). The present study presents the evidences for hapten-specific T cells and deals with their some properties. The spleen and lymphnode cells taken from PAB-MγG-primed mice could demonstrate helper activity for other haptenic group (2, 4-dinitrophenyl:DNP)-primed B cell in the presence of DNP-MγG-PAB to produce anti-DNP antibody. The helper activity of PAB-MγG-primed cells was hapten-specific, as MγG-primed cells could not demonstrate their helper activity. These hapten-specific helper cells belonged to T lymphocyte as judged by the result that the helper activity was abolished by in vivo ATS treatment. Helper activity of PAB-MγG-primed cells on anti-DNP antibody production was more weakly through DNP-keyhole limpet hemocyanin-PAB as compared with DNP-MγG-PAB. Accordinglythe specificity of helper cells were directed not only to the haptenic group but also to the hapten-conjugated carrier site. However, these helper cells could discriminate structural difference among related haptenic groups, because DNP-primed B cells were strongly stimulated with DNP-MγG-PAB in the presence of PAB-MγG-primed cells, but weakly in the presence of para-azobenzene sulphonate-MγG-primed cells. From the comparison between the specificity or T and B cells by the responsiveness to antigenic stimulation by related hapten-derivatives, the specificity of B cells was thought to be higher than that of T cells, because PAB-MγG-primed helper T cells were some what stimulated with related hapten-conjugated carrier (mata-azobenzoate:MAB-MγG), but PAB-MγG-primed B cells were not stimulated with MAB-MγG.View full abstractDownload PDF (1216K) -
Article type: Bibliography
1974 Volume 23 Issue 6 Pages 439-441
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (253K) -
Article type: Appendix
1974 Volume 23 Issue 6 Pages 442-
Published: June 30, 1974
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (168K)
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