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Volume 26, Issue 12
Displaying 1-15 of 15 articles from this issue
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Article type: Cover
1977 Volume 26 Issue 12 Pages Cover13-
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (194K) -
Article type: Cover
1977 Volume 26 Issue 12 Pages Cover14-
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (194K) -
Article type: Bibliography
1977 Volume 26 Issue 12 Pages Misc4-
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (241K) -
Toshihiko NemotoArticle type: Article
1977 Volume 26 Issue 12 Pages 793-802,838
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSEvaluation of the method for measurement of specific IgG by using protein-A Sepharose CL-4B was carried out(for the puropose of clinical application). The results obtained are as follows. 1) conjugation of IgG to protein-A Sepharose CL-4B is specific. This is confirmed by both affinity chromatography and immunoelectrophoresis method. 2) The maximum value of the specific IgG was observed when 50μl of serum was added to one ml of the protein-A Sepharose CL-4B comples. 3) Two inhibition tests were performed in order to determine whether the specific antibodies are truly measured or not. One was incubation with unlabeled insulin antigen and the other was incubation with unlabeled mite antigen. The results were compared respectively with those obtained with those with by the atandard methods. The results obtained with the inhibition tests were almost identical with those calculate theoretically which indicate the effect of inhibition. 4) Washing after incubation with <125>^I labeled antigen is necessary for 6 times. 5) a) The specific IgG antibodies to insulin are measured twice on twenty six sera. The correlation coefficient between the results of two measurements is 0.9885 is highly significant. (p<0.0001) b) Evaluation of technical error in measurement was carried out by doing duplicate tests on 24 samples of IgG to mite. Difference was within 10% and 5.9% in average. 6) Significant correlation coefficient was observed specific IgG and IgE antibody to egg white albumin (r=0.35, t=2.56, p<0.02). But no significant correlation was found in case of β-lactoglobulin.View full abstractDownload PDF (1288K) -
Toru Hikita, Shigeru Nakatani, Mitsuharu Kumai, Tetsuhiro Ninomiya, Yo ...Article type: Article
1977 Volume 26 Issue 12 Pages 803-807,838-83
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIt is well known that the increase of plasma cyclic AMP as a response to insulin-induced hypoglycemia is mediated by the release of endogenous adrenalin. This study was designed to find out if there was any difference in this cyclic AMP response to acute hypoglycemia between normal subjects and asthmatic patients. Regular insulin at the dose of 0.1 unit/kg of body weight was intravenously injected to 8 asthmatic patients (4 male and 4 female, age 14 to 55 years)and 5 normal male subjects (age 23 to 26 years). Blood samples were obtained before, 30, 60, 90 and 120 min after the injection of insulin, and were checked for blood glucose, plasma cortisol and cyclic AMP. Urine was collected for the period of 2 hours prior to and after the injection of insulin and assayed for catecholamines. There was no significant difference in the decrease of blood glucose and the change of plasma cortisol between two groups. No significant increase of urinary noradrenalin was observed in both groups, but urinary adrenalin was markedly increased with the mean increment of 6.16±3.42μg/2hrs and 4.41±3.6μg/2hrs in normal subjects and asthmatic patients, respectively, which had no significant difference between the 2 groups. Plasma cyclic AMP increased significantly from 5.8±3.3 p moles/ml to 13.3±3.5 p moles/ml(p<0.01)at 60 min in normal subjects, while it did not change significantly in asthmatic patients whose basal mean level was 6.2±2.7 p moles/ml. These results suggested that the response to insulin-induced hypoglycemia to increase plasma cyclic AMP was blunted and beta-receptors were suppressed in the studied patients with bronchial asthma.View full abstractDownload PDF (787K) -
Kyoko HayakawaArticle type: Article
1977 Volume 26 Issue 12 Pages 808-816,839
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSSome physiochemical and immunochemical properties of the suppressive T cell factor(TsF)extracted from carrier-primed thymocytes and spleen cells were investigated. Keyhole limpet hemocyanin(KLH)-specific TsF was successfully absorbed by an Immunoadsorbent com posed of KLH but not of unrelated antigen(Ascaris suum extract;Asc). The active component was recovered from the KLH-coated column by elution with 3M KCl. Despite this antigen-specificity. TsF was estimated to be between 35000 and 55000 daltons by Sephadex G-200 gel filtration. Since it has been established that TsF is a product of genes mapped in I-J subregion of major histocom-patibility complex, an attempt was made to characterize TsF by SDS-polyacrylamide gel electrophoresis after coprecipitation with anti-I-J alloantiserum and Staphylococcal protein A. The results, though not complete, suggested that TsF having both I-J determinants and the antigen binding site migrates to a position corresponding to a molecular weight around 44000 daltons under reducing condition.View full abstractDownload PDF (1305K) -
Yasuo Yui, Takaharu Shimizu, Yukiyoshi Yanagihara, Takao ShidaArticle type: Article
1977 Volume 26 Issue 12 Pages 817-826,839
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe cross-allergenicity of Compositae pollens was investigated using skin test. RAST, in vivo neutralization test of reaginic serum by P-K reaction, absorption of reaginic serum with solid-phase pollen antigens and agar gel diffusion. The results obtained were as follows. 1) The good correlation between skin test and RAST suggested strongly that the cross-allergenicity was found among Compositae pollens such as short ragweed, wormwood, goldenrod, chrysanthemum, dandelion. cocklebur, daisy, cosmos and marigold. 2) The cross-allergenicity between short ragweed and wormwood was observed in the minor allergen of these pollens. 3) The antigenicity of goldenrod was closer to that of wormwood as compared with that of short ragweed. 4) The results of skin test, RAST and agar gel diffusion using each rabbit anti-pollen extract serum suggested that the major allergen of wormwood was found to be identical with that of chrysanthemum. 5) The hypersensitivity reactions mediated by compositae pollens might result in the cross-allergenicity with either short ragweed or wormwood. However, the antigenicity of other Compositae pollens except for cocklebur was more closely related to that of wormwood than short ragweed.View full abstractDownload PDF (1471K) -
Tsugio Nakazawa, Takehisa Toyoda, Masahito Inazawa, Mitsuru Furukawa, ...Article type: Article
1977 Volume 26 Issue 12 Pages 827-831,840
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSShort-term sensitizing anaphylactic IgG antibody(IgG S-T S)reported by Parish is thought to be not a reagin and sensitize monkey skin for 2-4 hours to give PCA-like reaction. In order to investigate the biological activity of this antibody, monkey PCA reaction was performed using the heated sera of"Konjac bronchial asthma"patients and proper allergen. IgG S-T S antibody was detected in 21.5% of"Konjac asthma"but not detected in s case of "not Konjac asthma"and not any normal subjects exposed by the same allergen. PCA reaction induced by igG S-T S was weaker and paler than that by IgE antibody. Histological study of skin site of PCA reaction showed existance of intense edema and a few migrating cells in the layer of true skin. All the patients who had IgG S-T S also had IgE antibodies and they had not necessarily severe asthma.View full abstractDownload PDF (905K) -
Mitsuko Kogure, Yoshie Usuki, Sumie Satowa, Toshio Kawashima, Masae Mi ...Article type: Article
1977 Volume 26 Issue 12 Pages 832-837,840
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSPresent paper was studied relationship between complement system and cell-mediated system in the course of ageing. Six hundred and twenteen healthy subjects ranged from 10 to 94 years old were investigated, and the following results were obtained. The complement levels remain in elevated or normal ranges when cell-mediated immunity is depressed in the course of ageing. These phenomenon were explained that the complement system is elevated to compensate the impaired cell-mediated system to prevent the immunological surveillance system.View full abstractDownload PDF (727K) -
Article type: Bibliography
1977 Volume 26 Issue 12 Pages 838-840
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
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Article type: Appendix
1977 Volume 26 Issue 12 Pages 841-
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
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Article type: Index
1977 Volume 26 Issue 12 Pages 842-846
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (337K) -
Article type: Appendix
1977 Volume 26 Issue 12 Pages 847-
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
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Article type: Cover
1977 Volume 26 Issue 12 Pages Cover15-
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (36K) -
Article type: Cover
1977 Volume 26 Issue 12 Pages Cover16-
Published: December 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (36K)
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