All issues
Volume 26, Issue 6
Displaying 1-14 of 14 articles from this issue
- |<
- <
- 1
- >
- >|
-
Article type: Cover
1977 Volume 26 Issue 6 Pages Cover33-
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (40K) -
Article type: Cover
1977 Volume 26 Issue 6 Pages Cover34-
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (40K) -
Article type: Bibliography
1977 Volume 26 Issue 6 Pages Misc8-
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (280K) -
Satoru Oka, Moriyasu Tsuji, Toshihiko JyoArticle type: Article
1977 Volume 26 Issue 6 Pages 469-474,514
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSVarious antisera were prepared in rabbits by immunizing with the purified sea-squirt antigens, Gi-2 and Ei-2, and their acidic glycopeptide fragments, Gp and Ep, which were prepared from Gi-2 and Ei-2, respectively, by Pronase digestion. All of these antisera had affinities to ^<125>I-labeled Ei-2. Furthermore, the binding of the labeled Ei-2 to the antisera was significantly inhibited not only by the four antigenic preparations but also by the other two acidic glycopeptide fragments, Gp-A and Ep-A, which were prepared from Gp and Ep, respectively, by the NaOH-NaBH_4 treatment. These facts indicates that all of the six antigenic preparations were immunologically reactive with any one of the rabbit antisera. This agreed well with the previous observation that all of these preparations were antigenically active when they were examined by skin test in the asthmatic patients with sea-squirt allergy in vivo. Thus, the present results obtained in vitro supported the previous suggestion that the major components detected in both of Gp-A and Ep-A were the principal constituents of the antigenic determinant in sea-squirt antigens in common. However, the antigenic activities of Gi-2, Ei-2 and Gp were significantly higher than those of the other three. The fact suggested that the intact antigens and Gp carry another antigenic determinant in addition to the above-mentioned common determinant.View full abstractDownload PDF (579K) -
Tosisada KimuraArticle type: Article
1977 Volume 26 Issue 6 Pages 475-484,514-51
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSRice pollen is considered to be one of the important allergens in autumnal asthma in Japan. Specific hyposensitization treatment with rice pollen extract was carried out in 17 patients with rice pollen asthma, and the following results were obtained after hyposensitization. 1) Sixteen out of 17 cases ameliorated. 2) Out of 17 patients, threshold of intracutaneous testing decreased in 13, did not change in 3, and increased in 1. 3) P-K reaction changed to negative in 12 out of 15 patients, which persisted as long as 24 months. 4) In 14 patients, blocking antibody was detected to the amount of 1:4 to 1:1024 after the hyposensitization and was discernible within one year or so. 5) Increase of blocking antibody titer, especially diminution of P-K titer was parallel with the clinical improvement. From these results, it was concluded that specific hyposensitization treatment was effective in rice pollen asthma.View full abstractDownload PDF (1513K) -
Masato Nakayama, Seizo Yamana, Hideki Matsueda, Tadashi OfujiArticle type: Article
1977 Volume 26 Issue 6 Pages 485-489,515
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSColony inhibition factor derived from human lymphocyte was assessed by the in vitro technique. Lymphokine solution was prepared from resected tonsil lymphocytes, 3×10^6/ml, of children by adding PHA-P, 30 μg/ml. For colony inhibition assay (CIA), cultured mouse fibroblast suspension (L-929, 200 cells/ml), 1.5 ml were mixed with 1.5 ml of lymphokine solution and dispersed in Falcon plastic dish (No. 3002). Culture in CO_2 incubator was done for 6 to 9 days in the sterilized condition. Colonies formed on plastic dish were then stained, counted and expressed as % reduction=(No. control colony - No. experimental colony)×100/(No. control colony). In addition, preliminary application was carried out for the patient with SLE. Peripheral lymphocytes, 2×10^6/ml from the patient with SLE were incubated with 10 μg/ml of calf thymus native DNA. Two out of seven cases showed significant reduction than that of control group. The present data, therefore have suggested that the CLA might be utilized as a new in vitro assay for cell mediated immunity.View full abstractDownload PDF (716K) -
Katsumi SugiyamaArticle type: Article
1977 Volume 26 Issue 6 Pages 490-496,515
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESS1. Histamine release from isolated rat peritoneal mast cells induced by various histamine releasers, such as compound 48/80, sinomenine, ATP, concanavalin A, α-chymotrypsin and anti-rat mast cell rabbit serum was inhibited by papaverine in a dose-dependent manner. But the concentration of papaverine which inhibited the histamine release varied markedly with the histamine releaser. 2. Papaverine, in the absence of Ca^<++>, produced a maximum inhibition on compound 48/80- and sinomenine-induced histamine release, but this effect reduced with increasing Ca^<++> concentration. This reduction of papaverine action by Ca^<++> was overcome by prolonged incubation of cells with the drug. 3. Papaverine-induced inhibition of compound 48/80-induced histamine release was not affected by increasing the concentration of compound 48/80 and was rapidly reversed upon washing. 4. Cap formation induced by FITC-Con A of rat monocytes and Ehrlich ascites tumor cells was inhibited by papaverine and the membrane fluidity of liposomes and Ehrlich tumor cells was also reduced by papaverine. 5. The release of K^+ from human erythrocytes induced by lisolecithine was inhibited by paraverine. These results suggest that papaverine-induced inhibition of histamine release is probably due to the membrane stabilizing action.View full abstractDownload PDF (943K) -
Takako Nakazawa, Chiyuki Abe, Yuichi ShiokawaArticle type: Article
1977 Volume 26 Issue 6 Pages 497-503,516
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSMany investigators have reported that (NZB×NZW)F_1 hybrid (B/W) mouse produces many auto-antibodies and that the animal develops an autoimmune disease spontaneously. Studies were done on immune responses of the lymphoid organs such as spleen, bone marrow, lymphnodes and thymus using rosette formation test at 2, 4 and 8 months of age. Animals were devided into two groups, one group had immunization with sheep red blood cells (SRBC) 4 days prior to sacrifice and the other group had no immunization. B/W mice had hyper-responsiveness at very young age, but had rapid loss of the immune responses. However in B/W mouse bone marrow, non-immunized rosette forming cells (RFC) increased the number even at 8 months of age. C3H/He mice were used as controls, which had decreased both non-immunized and immunized RFC numbers with age in various organs studied. Anti-θ serum was prepared by injecting C3H/He thymocytes into AKR mice. After treating the cells with the anti-θ serum and guinea pig complement, the cells were used for RFC test. Then RFC were devided into two groups, such as θ positive RFC (T-RFC) and θ negative RFC (B-RFC). C3H/He mice had neither increase nor decrease of T-RFC/total-RFC ratio at each age studied in the various organs except non-immunized lymphnodes. B/W mice had more T-RFC than C3H/He mice in the spleen and bone marrow even at very young age, and had also stepwise increase of T-RFC/total-RFC ratio with age. It was the most interesting result that both the T-RFC number and T-RFC/total-RFC ratio increased in B/W mouse bone marrow with age. No explanation has been offered to affirm the characteristics of the T-RFC in the bone marrow. It is hoped that this kind of observation may lead to a satisfactory explanation on the pathogenesis of autoimmune disease.View full abstractDownload PDF (997K) -
Yoshio TakeuchiArticle type: Article
1977 Volume 26 Issue 6 Pages 504-510,516
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSPenicillin allergy depending on the degradation substances, products or immupurity substances in penicillin derivatives has been considered. In our previous experiment it was found that PcG polymer prepared in vitro had antigenicity to rats and activity eliciting PCA in rats. In order to detect PcG polymer having antigenicity, the experiment employing procain PcG suspension was carried out and the results obtained were as follows: Two methanol soluble fractions (65%) having Kav. 0.45 and 0.65 were eluted by gel filtration of the centrifuged supernatant of the suspension (147mg/l vial), of which molecular weights were estimated 4500 and 1000, respectively. Rf value of the substances determined by the thin layer chromatography were 0.41 and 0.55. From the structure analysis on the purified substances investigated by infra red spectroscopy it was estimated that beta lactam ring might be opened and it was also confirmed that it did not contain amino acid and 95mg of PcG polymer was detected from 3000000 units of procaine PcG suspension. It was clarified that over 99% specificity depending on PcG structure disappeared in various quantitative determination tests.View full abstractDownload PDF (1010K) -
Kenji Tominaga, Koichi Ogawa, Kentaro Yata, Eiro TsuburaArticle type: Article
1977 Volume 26 Issue 6 Pages 511-513,517
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSHypofunction of β-adrenergic receptor and hyperfunction of α-adrenergic receptor might affect the cyclic AMP and the cyclic GMP levels, respectively. There is some correlation between adenosine deaminase (ADA) activity and cyclic AMP level. ADA deaminates adenosine to inosine, and purine nucleoside phosphorylase (NP) converts inosine to hypoxantine, which is changed to IMP. These two enzymes are located in the salvage pathway of purine. We studied their activities in the lymphocytes from the patients with bronchial asthma. The tested samples were obtained from the patients who had neither asthmatic attack nor medication. ADA activities of lymphocytes from asthmatic patients were similar to those of healthy controls, whereas NP activities were significantly higher than that of controls. These results suspected that the higher activity of NP in the patients might be related to GMP levels and hyperfunction of α-adrenergic receptor in bronchial asthma.View full abstractDownload PDF (447K) -
Article type: Bibliography
1977 Volume 26 Issue 6 Pages 514-517
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (294K) -
Article type: Appendix
1977 Volume 26 Issue 6 Pages 518-520
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (203K) -
Article type: Cover
1977 Volume 26 Issue 6 Pages Cover35-
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (44K) -
Article type: Cover
1977 Volume 26 Issue 6 Pages Cover36-
Published: June 30, 1977
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (44K)
- |<
- <
- 1
- >
- >|