Japanese Journal of Allergology

[title in Japanese]

Article type: Cover
1980 Volume 29 Issue 12 Pages Cover10-
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.Cover10

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[title in Japanese]

Article type: Cover
1980 Volume 29 Issue 12 Pages Cover11-
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.Cover11

JOURNAL FREE ACCESS

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[title in Japanese]

Article type: Bibliography
1980 Volume 29 Issue 12 Pages Misc4-
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.Misc4

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THE IMPAIRMENTS OF LYMPHOCYTES INFLUENCED BY STIMULATED NEUTROPHILS FROM THE PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS AND RHEUMATIC ARTHRITIS

Yukie Niwa, Koichi Ishimoto, M Mitsuo Yokoyama

Article type: Article
1980 Volume 29 Issue 12 Pages 981-991
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.981

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The lymphocytes and neutrophils from the diseased groups or normal individuals were co-cultured at 37℃ for 17 hours, and the lymphocytes were again separatedly harvested and studied on their faculty of rosette formation and responsiveness to mitogens and antigen. Further, ^<51>Cr-release from the membrane of the lymphocytes given rise to by the neutrophils was also examined. In the lymphocytes from the normal individuals co-cultured with the neutrophils from SLE・RA groups, particulary in active stage, %T cell(E rosette formation)and T-γ cells were markedly reduced, EAC and Immunobeads rosette formations were slightly lowered. The responsiveness to Con A was remarkably depressed and that to PHA was mildly suppressed. Elevated ^<51>Cr-release was highly observed. The neutrophils from the patients with bacterial infections, lung cancer or normals could not influence on the lymphocytes(of the normals). The present study indicates that the decreased number of T cells and T-γ cells, and lowered suppressor function seen in SLE and RA might be attributable to the neutrophils which are stimulated in order to phagocytise the immune complex.

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QUANTITATIVE DETERMINATION OF SERUM HISTAMINE LEVELS IN ASTHMATIC PATIENTS BY HIGH- PERFORMANCE LIQUID CHROMATOGRAPHY

Hisanori Ezoe, Nobumi Sugita, Kiyoshi Furuichi, Toshihiko Sugihara, Ta ...

Article type: Article
1980 Volume 29 Issue 12 Pages 992-999
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.992

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HPLC analysis was found to be a very reproducible and specific method to determine a minute amount of histamine. It proved an effective analytical tool in investigating a relationship between serum histamine levels and a state of asthmatic paroxysm. In addition, it has wide applicability for investigations of in vivo and in vitro histamine release. Using this technique, we were able to show that the mean serum histamine level of healthy adults to be 1.31 ng/ml. In contrast, the mean histamine concentration of asthmatic patients was generally higher than that of healthy adults. Furthermore, the levels of histamine in patients during asthmatic attack showed a significantly higher mean value, 6.76 ng/ml, than those revealed in patients in a non-symptomatic state, 3.14 ng/ml, indicating that a positive correlation exists between high values of serum histamine and asthmatic attack.

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LONG-TERM CULTURE AND CLONING OF HUMAN T-LYMPHOCYTES

Shunichi Kato

Article type: Article
1980 Volume 29 Issue 12 Pages 1000-1007
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.1000

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Human alloreactive cytotoxic T-lymphocytes(HCTL)were maintained in a long-term culture, retaining function and specificity. By repeated addition of T cell growth factor(TCGF, Interleukin 2)obtained from supernatants of PHA stimulated peripheral blood lymphocytes, HCTLs grew continuously in vitro with a doubling time of 20 to 40 hours. During a 10 month culture period, these HCTLs have been cytotoxic but changed their specificity gradually, probably due to loss of some clones. Cloning of these HCTLs was successfully achieved by limiting dilution method. Primary clones were classified into several groups as follows; 1) Cytotoxic clones directed against classical HLA antigens. These are subdivided according to each HLA antigen involved. 2) Cytotoxic clones with unknown specificities. 3) Cytotoxic clones, mixture of 1) and 2). 4) Non-cytotoxic clones. Recloning of a primary clones(group 3)was done and revealed that this clone was not monoclonal, but a mixture of two cytotoxic clones. Although most of primary clones proved to be monoclonal, we found that the cloning procedure should be performed at least twice in order to obtain genuiness monoclonal T cell clones.

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MODULATION OF DELAYED TYPE HYPERSENSITIVITY BY HISTAMINE IN THE EFFECTOR PHASE

Kenji Tominaga

Article type: Article
1980 Volume 29 Issue 12 Pages 1008-1016
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.1008

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Recently, it is pointed out that some lymphocyte subsets have histamine receptors on their surface and these cells may regulate the immune response. The effects of histamine on the effector phase of delayed type hypersensitivity(DTH)induced by dinitrofluorobenzene(DNFB)in the mice were studied. Histamine suppressed DTH when the mice were sensitized with a high concentration(2.5%)of DNFB. On the contrary, DTH was enhanced when the mice were sensitized with a low concentration(0.1%)of DNFB. Modulation of DTH by histamine was mainly mediated by H_2 receptor. Adult thymectomy eliminated DTH modulation by histamine. These results indicated that histamine might modulate DTH through either suppressor amplifier T cells or DTH amplifier T cells. DTH was modulated by transferred spleen cells which were previously sensitized with DNFB and then treated with histamine in vitro

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STUDY OF IMMUNE RESPONSE SYSTEM IN MAN : Relationship Between HLA-D and DR Responsiveness to Candida Allergen

Yoshisuke Nose

Article type: Article
1980 Volume 29 Issue 12 Pages 1017-1027
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.1017

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The aim of this study was to investigate the relationship between HLA antigens, lymphocyte reaction and skin tests to Candida allergen and to delineate the existence of HLA-linked immune response genes in man. In vitro reaction to Candida allergen was studied in 110 normal healthy Japanese people in related to HLA-D and DR antigens. Significant associations were found between the low responder group and DR antigens. Significant associations were found between the low responder group and DEn(p<0.023)and MT2(p<0.017), and the high responder group and Dwl and Dr1(p<0.0005). In the reaction of HLA-D HTC to MCLC test, HLA-Dw1 proved to be in the high responder group. DEn was revealed to be in the low responder group. In vitro responsiveness to Candida allergen corresponded to skin test(r=0.884). In vitro activation of T-lymphocyte to Candida allergen was found to require the presence of autologus or allogeneic compatible macrophages. The study suggests the existence of immune response genes and immune response systems.

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EFFECT OF NEURAMINIDASE ON ANAPHYLACTIC CONTRACTION OF GUINEA PIG TAENIA COLI(I)

Tadao Okamura, Kazuhiro Shichinohe, Fumio Kimura, Fumiko Saito

Article type: Article
1980 Volume 29 Issue 12 Pages 1028-1034
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.1028

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Effect of neuraminidase on anaphylactic contraction has been studied using a guinea pig taenia coli preparation. After the pretreatment with neuraminidase, the contraction of smooth muscle provoked by the antigen added completely disappeared, while the same samples reacted well to histamine or acetylcholine. On the other hand, the pretreatment with neuraminidase produced significant influence on the estimates for Ca^<++>-and sialic acid-content in the smooth muscle tissues, with La-^<45>Ca space in anaphylaxis increasing and sialic acid decreasing. The results mentioned above indicate that neuraminidase may block the combination of antigen to cell membrane or may modify the utilization of the intracellular Ca^<++>. It has even been suggested that sialic acid on the cell membrane may act as a superficial Ca^<++>-source in the anaphylactic contraction of smooth muscle.

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[title in Japanese]

Article type: Index
1980 Volume 29 Issue 12 Pages 1035-1039
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.1035

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[title in Japanese]

Article type: Appendix
1980 Volume 29 Issue 12 Pages 1040-
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.1040

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[title in Japanese]

Article type: Cover
1980 Volume 29 Issue 12 Pages Cover12-
Published: December 30, 1980
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.29.Cover12

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