All issues
Volume 29, Issue 6
Displaying 1-11 of 11 articles from this issue
- |<
- <
- 1
- >
- >|
-
Article type: Cover
1980 Volume 29 Issue 6 Pages Cover25-
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (30K) -
Article type: Cover
1980 Volume 29 Issue 6 Pages Cover26-
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (30K) -
Article type: Bibliography
1980 Volume 29 Issue 6 Pages Misc9-
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (284K) -
Kazuhiko Muto, Masaharu Kasei, Shigeru Ito, Yoshihiro Oshida, Mikio Mu ...Article type: Article
1980 Volume 29 Issue 6 Pages 257-264
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSWe investigated the effect of culture supernatants from PHA-stimulated mononuclear cells on antigen (Dermatophagoides farinae)-induced histamine release from leukocytes in atopic patients. Histamine release was estimated by enzyme-isotopic assay. The antigen-induced histamine release was not affected by the addition of PHA and in the absence of antigen, the PHA-stimulated culture supernatants of mononuclear cells could not release histamine from leukocytes of atopic patients. As a whole, the culture supernatants of mononuclear cells stimulated with PHA of various concentrations, whether obtained from atopic patients or from non-atopic subjects, exerted similar enhancing activity on the antigenstimulated histamine release of atopic patients. However, when stimulated with 3μg/ml concentration of PHA-P, the culture supernatants from atopic patients showed a higher enhancing activity on histamine release than did those obtained from non-atopic controls (p<0.02). The maximum enhancing effect of culture supernatants seemed to be correlated with their RAST count to D.farinae (r=0.42, NS). These results suggest that under some conditions, soluble factors released from mononuclear cells of atopic patients may exert a pronounced enhancing effect on basophil histamine release by sensitized antigens as compared with those released from control mononuclear cells.View full abstractDownload PDF (984K) -
Michio Hiratani, Masaharu Kasei, Shigeru Ito, Yoshihiro Oshida, Mikio ...Article type: Article
1980 Volume 29 Issue 6 Pages 265-274
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIn vitro lymphocyte responsiveness and serum levels of specific IgE antibody to mite extract (Dermatophagoides farinae) and total IgE were evaluated in mite-sensitive patients and non-atopic individuals. Serum levels of both mite-specific IgE antibody and total IgE were significantly higher in mite-sensitive patients than in non-atopic individuals. The levels of specific IgE antibody well correlated with mite sensitivity. Lymphocyte from untreated mite-sensitive patients (n=16) responded to mite extract with greater ^3H-thymidine uptake than did those from non-atopic individuals (n=20)(p<0.01). In 42 subjects examined, including both mite-sensitive patients and non-atopic individuals, a significant correlation between lymphocyte response to mite extract and serum levels of mite-specific IgE antibody was observed (r=0.48, p<0.01). These results suggest that mite-sensitive patients respond to mite allergens with exaggerated lymphocyte proliferation as well as with enhanced specific IgE antibody production. To study the effect of immunotherapy, these three parameters were compared in patients receiving long term immunotherapy and those not receiving immunotherapy. The lymphocyte response to mite extract was significantly lower in the treated patients (p<0.05), but there was no significant difference in the total IgE and the specific IgE antibody levels between these two groups. These results suggest that with regard to mite-allergy, there is some difference in the effect of immunotherapy between lymphocyte proliferative response and serum levels of specific IgE antibody to mite allergen.View full abstractDownload PDF (1133K) -
Shigeru IshidaArticle type: Article
1980 Volume 29 Issue 6 Pages 275-285
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSFemale BALB/c mice were s.c. injected with 10μl turpentine oil and changes in plasma Ss protein levels were determined by a single radial immunodiffusion test. The level of Ss protein markedly increased reaching at least double the amount in normal mouse plasma. Cross-immunoelectrophoretic analysis of either plasma or serum showed that the mobility of Ss protein in the serum of turpentine-treated mice is more in the anodal region than that of plasma and that Ss protein in plasma or serum moved to the same region as that of normal mouse plasma or serum. This indicates that the increased Ss protein 72hr after the injection of turpentine oil is a non-activated form of Ss protein and can form the complex with C4-binding protein, even if the serum is drived from turpentinetreated mice. Next we examined whether the observed elevation of Ss protein reflected the enhancement of biosynthesis and secretion of Ss protein in vivo. Colchicine was i.v. injected into BALB/c mice 3 days before, on the same day, one or 2 days after the injection of turpentine oil, and Ss protein levels were determined 72hr after the injection of turpentine oil. The elevation of Ss protein level was completely i.v. with puromycin before or after the injection of turpentine oil did not show any increase in the level of plasma Ss protein. This inhibitory effect of puromycin was also observed when it was simultaneously injected with the turpentine oil. Furthermore, the level of plasma Ss protein in mice injected with puromycin alone decreased to approximately 30% of the normal level. Our results indicate that the elevation of plasma Ss protein level in mice 72hr after the inflammatory reaction depends on the secretion of newly synthesized Ss protein.View full abstractDownload PDF (1669K) -
Akihiro Morikawa, Shigeyoshi SuzukiArticle type: Article
1980 Volume 29 Issue 6 Pages 286-292
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSWe studied the effect of thymosin fraction V-1 and V-2 (Thy.Fr.V-1 and V-2) on cyclic nucleotides in human lymphocytes. 1) The basal level of cAMP in cord blood lymphocytes was 5 to 10 times higher than that in peripheral blood lymphocyte (PBL), while cGMP was almost the same as PBL. 2) After 1-min incubation with 100μg of Thy.Fr.V-1, cAMP in PBL was significantly elevated, however, cAMP in cord blood lymphocytes was not changed. 3) Cyclic GMP in both PBL and cord blood lymphocytes was not changed by the incubation with Thy.Fr.V-1. 4) Different from our previous finding that Thy.Fr.V-land V-2 have an enhancing and inhibitory activity on E-rosette formation of PBL, respectively, both the fractions increased cAMP in PBL. 5) From the result that Thy.Fr.V-1 further increased cAMP in PBL when phosphodiesterase was completely blocked by isobutyl methyl xanthine (IBMX), it was concluded that Thy.Fr.V-1 has at least the ability to stimulate adenylate cyclase in PBL.View full abstractDownload PDF (825K) -
Yukimasa SawadaArticle type: Article
1980 Volume 29 Issue 6 Pages 293-305
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSOf 218 adult apple farmers examined in an epidemiological survey on apple pollinosis, 5 (2.3%) were diagnosed as having the disease, based on the following criteria: A possibly or definitely positive skin test, positive Prausnitz-Kustner (PK) reaction and nasal provocation tests and a serum specific IgE level score of 1 to 3 by radioallergosorbent test (RAST). The incidence of apple pollinosis has been estimated by statistical data analysis to be 0.4 to 4.2% in the entire population of apple farmers, with a 95% confidence limit. The 5 patients were followed up by periodic check-ups during and after the artificial pollination period. The RAST count which initially was low became markedly elevated during this period, followed by a brief decline. Thereafter it tended to remain at a level higher than the initial (pre-exposure) level in 3 of these cases. Furthermore there was a highly significant correlation with the end-point of the reaction by the skin test and it was consistent with the PK reaction and nasal provocation tests during the period of artificial pollination. The serum total IgE level by the radioimmunosorbent test (RIST) and peripheral blood eosinophil count increased during or after the artificial pollination period.View full abstractDownload PDF (1172K) -
Fumiaki Onishi, Takashi Shiba, Toshihiro Higashimori, Yasuhiro Mizoguc ...Article type: Article
1980 Volume 29 Issue 6 Pages 306-317
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe peripheral lymphocytes from patients with drug-induced allergic intrahepatic cholestasis were stimulated in vitro with the causative drug in the presence of carrier protein. Culture supernatant of the activated lymphocytes was then slowly infused via the mesenteric vein of rats for 3 hours. Bile flow and the excretion of bile acids were reduced remarkably in a time-dependent manner following the infusion. The serum concentration of total bile acids and the activity of enzymes derived from the biliary tract were increased after the infusion. Electron micrographs of rat hepatocytes after the infusion exhibited the characteristics of cholestasis in that the bile canaliculi were dilated with diminution of microvilli. Moreover, when the lymph node cells of tuberculin-sensitized guinea pigs were stimulated with purified protein derivative and the culture supernatant was then infused via the rat mesenteric vein, similar intrahepatic cholestasis could be induced. These findings suggest that there may be a lymphokine which exerts cholestatic effect (cholestaic factor). This factor may be produced from the sensitized lymphocytes by activation with the drugcarrier or antigen and may play an important role in inducing the intrahepatic cholestasis frequently observed in drug-induced allergic hepatitis. This factor may resemble the macrophage migration inhibitory factor (MIF) in some aspects.View full abstractDownload PDF (1822K) -
Article type: Appendix
1980 Volume 29 Issue 6 Pages 318-321
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (303K) -
Article type: Cover
1980 Volume 29 Issue 6 Pages Cover27-
Published: June 30, 1980
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (230K)
- |<
- <
- 1
- >
- >|