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Volume 30, Issue 6
Displaying 1-15 of 15 articles from this issue
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Article type: Cover
1981 Volume 30 Issue 6 Pages Cover24-
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (37K) -
Article type: Cover
1981 Volume 30 Issue 6 Pages Cover25-
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (37K) -
Article type: Bibliography
1981 Volume 30 Issue 6 Pages Misc9-
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
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Naomi Kondo, Tadao Orii, Hisao UetakeArticle type: Article
1981 Volume 30 Issue 6 Pages 281-288
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSPokeweed mitogen (PWM)-induced polyclonal immunoglobulin (Ig) production of B cells is highly reproducible and the response has been shown to be dependent on T cells. Using T and B cells obtained from healthy individuals and patients with immunodeficiency, we investigated whether or not human helper T cells are able to collaborate with B cells in PWM-induced polyclonal Ig production across allogeneic barriers. It has become clear in our results that the B cell competence is required for T cell help in human lymphocytes. In other words, our results suggest that T cell help in PWM-induced Ig secretion of B cells may be restricted by allogeneic barriers. They also imply that in order to investigate the T cell helper activities of a (hypo)-gammaglobulinemia patients, their T cells should be tested in co-cultures not only with B cells of the third-party individuals but also with B cells of family members of the patients.View full abstractDownload PDF (928K) -
Shinzo Uchida, Kogi Satomi, Hajime Imamura, Hiroshi Inaba, Toshikazu N ...Article type: Article
1981 Volume 30 Issue 6 Pages 289-294
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThree different exercise tolerance tests for asthmatic children were examined. Subjects: 25 EIA positive asthmatic children (18 male, 7 female). Methods: 1)Treadmill, 10 degree, 6 km/hr, 6 min. 2)Cycle ergometer, 300W. 3)Master one step, 24/min, 6 min. Results: No difference was found among "treadmill group", "cycle ergometer group" and "master one step group" in pulse rate, blood pressure (systolic and diastolic), FVC, FEV_<1.0>, and PFR. These results indicate that the master one step method is as useful as other exercise tolerance tests for examining EIA.View full abstractDownload PDF (706K) -
Junzaburo Kabe, Hirosuke NakazawaArticle type: Article
1981 Volume 30 Issue 6 Pages 295-300
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIt has been shown that asthmatic patients have increased bronchial reactivity to acetylcholine (Ach) and the respiratory threshold to inhaled Ach is very low. The threshold is known to be affected by the bronchodilators, antihistamines, anti-cholinergics, etc. But no significant change is seen after short-term treatment of less than 1 to 2 yrs when the experiment is performed with these drugs being withheld for more than 12 hours prior to it. This study was designed to determine whether bronchial hyperreactivity of asthmatic patients changes during the long-term treatment and whether it may be lowered to the level of normal subjects after a symptom-free period of several years. Inhalative Ach test was carried out on 17 asthmatic patients repeatedly, either 2 or 3 times respectively, over a period of 6 months to 8 years a total of 42 occasions. Subjects were asked to inhale an aerosol of Ach solution with increased concentration for 3 minutes, and the minimum dosed of Ach used to cause the initial decrease of FEV_<1.0> was defined as threshold of Ach. The threshold of these subjectsl was low and the degree of response to Ach appeared to be proportional to the severity of their asthma. No apparent difference was found between the reactivity and the type of patients's asthma. After treatment of 6 to 12 months, the threshold did not change significantly. After 3 to 6 yrs observation, a trend toward lowered reactivity was noted in patients whose asthma had been improved. The increased responsiveness to Ach may persist many years after asthmatic symptoms have ceased. Two patients who had been symptom-free for more than 3 yrs reacted much less intensely than they did in the former experiment performed at the asthmatic period, but were still hyperreactive to Ach compared to the normal subjects.View full abstractDownload PDF (792K) -
Michiko Haida, Maki Hasegawa, Nobukazu Yamauchi, Tadaatsu Ogita, Hirok ...Article type: Article
1981 Volume 30 Issue 6 Pages 301-307
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSAnalysis of the house dust mite (Dermatophagoides farinae) allergens were performed by radioimmunoelectrophoresis using ^<125>I labeled anti-IgE antibody. Extract of isolated Dermatophagoides farinae was reacted with 33 atopic patients' sera, and 4 different radioactive bands were identified. One patient serum (H. I. ) showed 2 precipitin bands. Two corresponding antibodies were precipitated by 33% saturation of ammonium sulphate and assumed to be associated with IgG antibody. One of the 2 antigens reactive with the precipitating antibody was also reactive with IgE antibody. Collectively, 6 different mite antigens were identified in the present study. Major allergen was determined both on the intensity of the radioactive bands and on the frequency of detection in the patients' sera. One allergen with the slowest electrophoretic mobility (Mε1) was assigned to be the major allergen.View full abstractDownload PDF (1584K) -
Motohiro Kurosawa, Toshikazu Nimoto, Tetsuji Tanaka, Ryuzo Fueki, Sets ...Article type: Article
1981 Volume 30 Issue 6 Pages 308-315
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSProtein kinase (PKase) activity in human leukocytes was measured and the relation between anti-IgE-mediated histamine release and PKase was investigate. Leukocyte preparation was obtained with a 6% dextran solution and red blood cells were removed with 0.83% NH_4Cl treatment. The following results were obtained. 1. PKase activity was observed in human leukocytes, but there was no significant elevation of the activity with administration of cyclic AMP (cAMP). 2. The amount of histamine release was dependent upon the concentration of anti-IgE and was enhanced by incubation with EDTA-heparin solution and reaginic serum from the patient. 3. When histamine release was observed, it was found that PKase activity, measured with cAMP or without cAMP, was reduced irrespective of EDTA, heparin and reaginic serum treatment. 4. Histamine release and the decrease of PKase activity were inhibited by the addition of N-5', isoproterenol and theophylline to incubation mixture. From the above results, it is clear that PKase exists in human leukocytes, and it seems that PKase may play an important role in the mechanism of histamine release from human leukocytes.View full abstractDownload PDF (1025K) -
Yasushi YukiyamaArticle type: Article
1981 Volume 30 Issue 6 Pages 316-323
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe method of functional assay of factor B(B) in human serum was investigated. Cobra venom factor (CVF), which specifically binds B, was purified from crude cobra venom by sequential column chromatography and electrofocussing according to Ballow and Cochrane (1969) and to Lachman et al. (1976). Two tenth ml of samples and an equal volume of CVF were incubated at 37℃ in the presence of Mg-EGTA for 60 min, and then half ml of guinea pig erythrocytes in 0.02 M EDTA. VBS and 0.25 ml of human C-EDTA were added. After 60 min at 37℃, the grade of hemolysis, which correlated with the B activity was determined. These reactions were inhibited neither by anti-IgG nor anti C3 nor anti C4, but inhibited by anti B. Factor D(D), which is an essential factor in this assay system, was not a limitting factor because addition of 50℃ heated serum as a source of D did not remarkably enhance the extent of hemolysis. However, to prevent a possible restriction by a shortage of D, 50℃ heated serum may be added as an improved method. The value of B determined by CVF hemolysis assay were well correlated with those by Mancini method (r=0.92).View full abstractDownload PDF (845K) -
Article type: Appendix
1981 Volume 30 Issue 6 Pages 325-
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
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Article type: Appendix
1981 Volume 30 Issue 6 Pages 326-327
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
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Article type: Appendix
1981 Volume 30 Issue 6 Pages 328-
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
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[in Japanese]Article type: Article
1981 Volume 30 Issue 6 Pages 329-
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
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Article type: Appendix
1981 Volume 30 Issue 6 Pages 331-334
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
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Article type: Cover
1981 Volume 30 Issue 6 Pages Cover26-
Published: June 30, 1981
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (816K)
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