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Volume 31, Issue 12
Displaying 1-16 of 16 articles from this issue
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Article type: Cover
1982 Volume 31 Issue 12 Pages Cover10-
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (42K) -
Article type: Cover
1982 Volume 31 Issue 12 Pages Cover11-
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (42K) -
Article type: Bibliography
1982 Volume 31 Issue 12 Pages Misc4-
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (393K) -
Kazuo Sugimoto, Tsuyoshi Toba, Hisao Tomioka, Makoto Tanaka, Akira Kum ...Article type: Article
1982 Volume 31 Issue 12 Pages 1167-1176
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDynamic change of respiratory resistance and <81m>^Kr ventilation image on the methacholine provocation test were simultaneously monitored by both the oscillation method using 'Astograph (TCK-6100 H)' and <81m>^Kr gas inhalation in 12 atopic patients (6 adults and 6 children) and 6 normal individuals (4 adults and 2 children). Concommitant changes in the initiation of increase in the respiratory resistance and the start of ventilation image defect were clearly observed, especially in the adult cases. In the cases of younger-aged children, it was difficult to judge the initiation of increase in the respiratory resistance tested by 'Astograph' due to initial high level and wide fluctuation of respiratory resistance. Therefore, to study bronchial hyperreactivity in the children the image of <81m>^Kr gas inhalation was more reliable than the test by 'Astograph'. Another advantage of the <81m>^Kr ventilation image was that it could detect the area of change in the lung fields. The monitoring of the respiratory resistance by the oscillation method and/or <81m>^Kr ventilation image on bronchial provocation test might give us some new information to understand the bronchial hyperreactivity.View full abstractDownload PDF (1106K) -
Yohji Nagai, Siroh Kikuchi, Takanori Hirayama, Jun-ichi Hirao, Isao Se ...Article type: Article
1982 Volume 31 Issue 12 Pages 1177-1186
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSWe studied sterol biosynthesis and DNA synthesis in human peripheral mononuclear cell (PBMs), T cell enriched fraction, B cell enriched fraction and monocyte enriched fraction activated by various mitogens. l) The sterol biosynthesis was maximally accentuated at 36-48 hr from the start of culture in T cell mitogen activated PBMs, at 4 day in PWM activated PBMs and at 5 day in LPS activated PBMs, reapectively. And, the peak of sterol biosynthesis was earlier than that of DNA synthesis in all cases. 2) In the activation by T cell mitogens, sterol biosynthesis was accentuated in the T cell enriched and monocyte enriched fractions. And, in the former, DNA synthesis was accompanied and therefore, activated T cells were major factor of sterol biosynthesis. But, in the latter, DNA synthesis was not followed. So, it would be probable that sterol biosynthesis of monocytes is greater if they are activated by T cell mitogens. 3) In the activation by PWM and LPS, sterol biosynthesis in B cell enriched and monocyte enriched fractions was accentuated. And, in the former, DNA synthesis was followed. So, it would be that sterol biosynthesis of activated B cells is greater. And, in the monocyte enriched fraction, DNA synthesis was accepted. Therfore, it would be that in those fractions, activated B cells in place of monocytes are major factors of accentuation of sterol biosynthesis.View full abstractDownload PDF (1191K) -
Masaharu TsudaArticle type: Article
1982 Volume 31 Issue 12 Pages 1187-1193
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSCirculating complexed IgE was investigated by gel filtration using Sephacryl S-300 superfine at both pH 8.0 and 3.2 in serum from a patient with bronchial asthma complicated by atopic dermatitis. In 97 cases, ranging from 3 to 13 years of age, serum IgE and 4% polyethylene glycol precipitated IgE levels were measured by paper radioimmunosorbent test. The rate of 4% polyethylene glycol precipitated IgE to serum IgE was calculated as PEG precipitated index (PP index) to exclude the effect of serum IgE levels. The following results were obtained: 1) Serum from a patient with bronchial asthma complicated by atopic dermatitis was fractionated by borate buffered saline, pH 8.0. Two peaks of IgE in monomeric and complexed or aggregated form were demonstrated. Next, serum from the same patient was fractinated by glycine HCI buffer, pH 3.2, in investigating macromolecular IgE, the complexed or aggregated form. The first peak of IgE in complexed or aggregated form by gel filtratiop at pH 8.0 was dissociated by filtration at pH 3.2. These results suggest that IgE immune complex exists in serum from patients with bronchial asthma complicated by atopic dermatitis. 2) In the bronchial asthma patients, IgE PP indices were at high levels compared with control subjects, but IgG and IgA PP indices were almost within normal range. 3) IgE PP indices in asthmatic children with attack were at significantly higher levels than in those without attack. These results suggest that circulating IgE immune complex may play etiological role in the pathogenesis of bronchial asthma.View full abstractDownload PDF (764K) -
Masako Okawa, Shinichi Aotsuka, [in Japanese], Ryuichi YokohariArticle type: Article
1982 Volume 31 Issue 12 Pages 1194-1204
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIn vitro spontaneous synthesis of immunoglobulins and anti-single-stranded DNA antibodies (anti-ssDNA) by mononuclear cells (MNC) and monocyte-depleted MNC was examined in patients with systemic lupus erythematosus (SLE) and compared with that in normal individuals. MNC were isolated by Ficoll-Hypaque density gradient centrifugation. Monocytes were depleted by treatment with thrombin. MNC were cultured for 7 days. The culture supernatants were measured for IgG and IgM by enzyme-linked immunosorbent assay and for anti-ssDNA by solid phase redioimmunoassay, respectively. In SLE, significantly increased synthesis of immunoglobulins by MNC was reduced when monocytes were depleted. In the normal individuals, depletion of monocytes tended to increase synthesis of IgG and IgM. SLE monocytes seemed to play a helper role in synthesis of IgG and IgM by MNC, while normal monocytes seemed to play a supperssor role. Levels of anti-ssDNA of either IgG or IgM class in the culture supernatant correlated well with those in sera obtained at the time when MNC were examined. These findings indicating preference that studies on in vitro synthesis of specific autibodies are useful in elucidating the mechanism of autoantibody production in vivo.View full abstractDownload PDF (1187K) -
Yoshihisa Sato, Toshiaki KobayashiArticle type: Article
1982 Volume 31 Issue 12 Pages 1205-1214
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe contact allergenicity of lanolin was investigated by means of a modified technique of guinea pig testing which involved the use of the complete Freund's adjuvant and external application of the test compound in the induction stage. Only lanolin alcohol (BP) produced a marked positive reaction, but other lanolin derivatives, consisting mainly of wax esters, did not produce a positive allergic reaction at any challenge concentration. The allergens in lanolin alcohol, which dissolved in methanol or acetone, turned out to be multiplex components with wide range of polarity. Aliphatic alcohols and aliphatic diols, which had been reported to be possible allergens of lanolin, did not produce a positive reaction. Lanolin alcohol was fractionated with high perfornance liquid chromatography, and the fractionated materials which gave strong positive reactions were studied by 13^C-NMR and GC-MS. The results of chemical analysis suggest that the allergens in lanolin alcohol consist of a compounds with 2 or 3 hydroxy groups, double bond groups and a ketone group.View full abstractDownload PDF (1094K) -
Shinichi Tomioka, Takao Tochigi, Munehiro Arai, Masahito Inazawa, Ryuz ...Article type: Article
1982 Volume 31 Issue 12 Pages 1215-1221
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSNifesipine, a potent calcium antagonist, was given to 33 asthmatic patients, in order to investigate its possible effect on bronchoconstriction. 10 mg of nifedipine with a bronchodilator, i.e., 4mg of terbutaline, was given orally to group A(9 cases); placebo of nifedipine and terbutaline to group B(8 cases); nifedipine with placebo of terbutaline to group C(8 cases); and placebos of both drugs to group D(8 cases). Medication of the subjects was carried out using a double blind method, and all other drugs were washed out the subjects 16 hours or more before the medication. In order to confirm the effect on pulmonary function, forced expiratory spirograms and maximal expiratory flow volume curves were examined before, and after 15 min, 30 min and 60 min of the medication. The results were as follows: 1. In group D, in which placebos were given, parameters of ventilatory function gradually and significantly decreased from the baseline values following the time course. 2. However, in group C, nifedipine apparently inhibited this decrease in ventilatory function. 3. In groups A and B, i.e., those in which terbutaline or terbutaline with nifedipine were given, parameters of ventilatory function significantly increased in comparison with the baseline values, although there was no apparent advantage of combination of the two drugs over use of terbutaline alone. 4. In group D, pulse rate significantly decreased from baseline value; it remained unchanged in both groups A and B, and increased in group C. Blood pressure apparently fell from baseline value in both groups A and C. The above results suggest that the calcium antagonist nifedipine inhibits bronchoconstriction in asthmatics, probably due to vagotony induced through repeated deep inspiration and expiration. This would also support the reported effect of the drug on exercise-induced asthma.View full abstractDownload PDF (941K) -
Reiko Kishikawa, Hitoshi NaganoArticle type: Article
1982 Volume 31 Issue 12 Pages 1222-1230
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe distribution and the nature of airborne pollens in Japan were investigated on a nation wide scale by the staffs of abour 50 hospitals and sanatoriums from 1975 to 1977, supported by the Welfare Ministry of Japan. This investigation was subsequently continued to 1981 at 11 institutions. Based on these investigations, we report the nature of the airborne pollen and also the results of meterological investigation of cedar pollens using Durham's standard pollen sampler method. The yearly changes in number of the important pollens were examined by preparing pollen calenders, pollen maps and Pollen Front and Pollen Last which mean first and last appearance of the pollens Among pollen grains we identified every year were cedar, cypress, pine, birch, beech, grass, ragweed, mugwort , and Japanese hops. The ceder pollen count changes remarkably every year because the climate conditions of the previuos summer determine the count of the pollen the next year. High temperature and low humidity in summer increase the pollen count the next year. Ceder also begins to pollinate whenever the temperature goes up suddenly in spring.View full abstractDownload PDF (1133K) -
Yukiko ItoArticle type: Article
1982 Volume 31 Issue 12 Pages 1231-1238
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSA radioimmunoassay with protein A-Sepharose CL-4B, described by Foucard et al., was carried out to measure IgG antibody to Japanese cedar pollen antigens. Crude extract of the pollen was fractionated by DEAE-cellulose chromatography to separate major allergenic components. Characterization of each fraction showed the following results: major allergenic components were present in fraction 2, which was revealed by RAST inhibition; these major allergens were about 38,000 daltons and 40,000 daltons by SDS-polyacrylamide gel electrophoresis. IgG antibody was measured by the radioimmunoassay using each fraction labelled with 125^I; the highest IgG antibody in undiluted serum was detected when 125^I-fraction 2 was used. These results indicate that radioimmunoassay with protein A-Sepharose CL-4B used in conjunction with 125^I-major allergens from Japanese cedar pollen antigens is useful method for measurement of specific IgG antibody. It could be applicable in various fields of clinical studies on cedar pollenosis.View full abstractDownload PDF (1208K) -
Hisao TomiokaArticle type: Article
1982 Volume 31 Issue 12 Pages 1239-1243
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSSynthetic leukotriene thioether, leukotriene C_4 (LTC_4) and D_4 (LTD_4) which do not have sulfate in their chemical structure being hydrolyzed by arylsulfatase, were subjected to the enzyme reaction with purified human placental arylsulfatase B (AS-B), and limpet arylsulfatases type IV (ASL type IV) and V (ASL type V). The biological activity remaining was measured by bioassay using guinea pig ileum, and the degradation products were identified by high pressure liquid chromatography (HPLC). Neither LTC_4 nor LTD_4 were inactivated by human placental AS-B. Type IV ASL inactivated both LTC_4 and LTD_4. It was also demonstrated by HPLC analysis that type IV ASL convcrted LTC_4 into LTD_4 and LTE_4, and LTD_4 into LTE_4. However, type V ASL inactivated only LTD_4, but not LTC_4. LTD_4 was converted to LTE_4 by type V ASL. It can be assumed that type IV ASL might contain at least two enzymes, each of which could convert LTC_4 into LTD_4, and LTD_4 into LTE_4, whereas type V ASL might contain one enzyme, which is able to convert LTD_4 into LTE_4.View full abstractDownload PDF (456K) -
[in Japanese]Article type: Article
1982 Volume 31 Issue 12 Pages 1244-1245
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
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Article type: Index
1982 Volume 31 Issue 12 Pages 1246-1251
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
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Article type: Appendix
1982 Volume 31 Issue 12 Pages 1252-1257
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
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Article type: Cover
1982 Volume 31 Issue 12 Pages Cover12-
Published: December 30, 1982
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (309K)
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