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Volume 33, Issue 2
Displaying 1-13 of 13 articles from this issue
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Article type: Cover
1984 Volume 33 Issue 2 Pages Cover12-
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (322K) -
Article type: Cover
1984 Volume 33 Issue 2 Pages Cover13-
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (322K) -
Article type: Bibliography
1984 Volume 33 Issue 2 Pages Misc5-
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (227K) -
Hirokuni Ohtsuka, Minoru Okuda, Yoshikiyo Sakaguchi, Masaki Ohnishi, T ...Article type: Article
1984 Volume 33 Issue 2 Pages 71-77
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIn 1979, Nalebuff and his coworkers modified the ordinary Phadebas RAST in the points as follows: the volume of test serum was increased from 50 to 100μl; the duration of the first incubation period was lengthened from 3 hours to 18 hours, and the tragged disc transferred to fresh tubes prior to placing them into the gamma counter. We compared the Phadebas RAST assay with the modified RAST assay using the serum of patients sensitive and non-hyposentitized to house dust and Japanese cedar pollinosis. We found better correspondence between RAST and skin or provocation reaction was in the modified RAST than in the Phadebas RAST. This was possibly caused by an increase of sensitivity as a result of prolonging the time of incubation of test serum with antigen.View full abstractDownload PDF (728K) -
Kazuo Tanifuji, Ataru Daibo, Morio Sudo, Masao Tamura, Toshihiko Jyo, ...Article type: Article
1984 Volume 33 Issue 2 Pages 78-82
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIn vitro histamine release tests with four types of the sea-squirt antigens were performed in a patient with sea-squirt asthma. These antigens were the crude antigen and the three purified types of antigens, D (molecular weight of 10,000), Gi-rep(110,000) and EiM (23,000). The results were as follows; Maximal histamine release with crude antigen, purified D antigen and Gi-rep antigen was observed at concentrations of 20, 10^2 and 10^3, respectively. The percent of histamine release after challenge with crude, D and Gi-rep antigens decreased in proportion to the dilution of antigen. Maximal histamine release with EiM antigen was observed at the concentration of 10^2, but the percent of histamine release was not different at concentrations of 10^2 and 10^3, and decreased in proportion to the dilution of antigen. It was shown that the histamine release with the four types of the sea-squirt antigens was proportional to the concentration of these antigens.View full abstractDownload PDF (610K) -
Yoshiro Tanizaki, Toshio Sato, Yutaro Shioda, Yoko Matsuka, Katsumi Ak ...Article type: Article
1984 Volume 33 Issue 2 Pages 83-87
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSChemotaxis of blood monocytes to zymosan activated serum was examined in 31 patients with bronchial asthma, using agarose plate method. 1. In a study on migration value of blood monocytes from healthy subjects, the chemotaxis (CT) was 0.74±0.04mm, the random migration (RM) 0.41±0.03mm and chemotactic differential (CD) 0.33±0.03mm. 2. The migration values of blood monocytes from the patients with bronchial asthma were almost the same, in non-attack and attack stages, as those from healthy subjects. In post-attack stages of bronchial asthma, the migration values such as CT, RM and CD, were all higher than those of healthy subjects: CT 1.66±0.17mm, RM 0.99±0.14mm and CD 0.67±0.07mm. 3. The CT and RM of blood monocytes from the cases with serum IgE levels over 801IU/ml were significantly lower than those from the cases with a level of less than 500IU/ml. There was no significant difference in the CD between the two groups. 4. There was no significant difference in migration value of blood monocytes between the cases with and without glucocorticoid therapy.View full abstractDownload PDF (644K) -
Rhuji AsaiArticle type: Article
1984 Volume 33 Issue 2 Pages 88-95
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIntravenous injection of MMC treated spleen cells (SC) elicted proliferative responses in SC of syngeneic recipient mice. The activated SC augmented in vitro DNA synthesis to three times that of intact SC from noninjected mice. The responses at 3rd day of culture were higher than 6th day of culture. However, the responses of the SC treated with anti-Thy-1.2 and anti-Lyt-1 monoclonal antibodies plus complement decreased significantly, but no such decrease occurred with treatment of anti-Lyt-2 monoclonal antibody plus complement. The stimulation index of in vivo activated T cells to normal T cells was higher at 2nd day of culture with MMC treated syngeneic SC than with MMC treated allogeneic SC, but no difference was observed at 4th day of culture. Moreover, the increase in in vitro DNA synthesis was reduced significantly by the removal of in vivo activated T cells adhering to syngeneic splenic adherent cells (SAC) monolayer, but not by the removal of those adhering to allogeneic monolayer. The responses of host SC to injection of MMC treated syngeneic SC had specificity and memory, suggesting that this response may be immunological in nature. Therefore, this response may be called in vivo autologous MLR (aMLR). Further studies on the role of in vivo aMLR are now under study.View full abstractDownload PDF (990K) -
Toshiroh Sugiyama, Kohzoh Imai, Yasuo Moriya, Tetsuo Sasanami, Akira F ...Article type: Article
1984 Volume 33 Issue 2 Pages 96-101
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSTwo hybridomas derived from murine myeloma cells (X63-Ag8-653) which were fused with splenocytes of mice immunized with human histiocytic lymphoma cell line U937, secreted monoclonal antibodies that reacted with antigenic determinants preferentially expressed in human monocytes in long-term culture. Monoclonal IgG2b antibody UH316 reacted with 66±19% of human monocytes, and 32±2% of human T cells, but not with B cells or neutrophils. Monoclonal IgG1 antibody UD916 reacted with 71±16% of human monocytes, but not with human T cells, B cells or neutrophils. The monoclonal antibody UH316 was found to react with peripheral mononuclear cells, which show NK activity against K562 target cells. On the other hand, the monoclonal antibody UD916 did not react with NK cells. This antibody, however, was able to identify a monocyte subset which plays a role in the helper function for proliferation of mitogen-induced T cells.View full abstractDownload PDF (699K) -
Hiroyuki Inui, Toshikazu Nagakura, Yoji IikuraArticle type: Article
1984 Volume 33 Issue 2 Pages 102-111
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSExercise tests by ergometer were performed on asthmatic children with exercise-induced late asthmatic response (EILAR). Plasma histamine and neurtrophil chemotactic factor (NCF) were meassured. The exercise tests were also performed with mask and after administration of DCSG and salbutamol inhaler. Lung function was measured for 9 hr after exercise tests. Our findings were follows: 1. Plasma histamine did not change in EILAR phase, but NCF increased in both exercise-induced early asthmatic response (EIEAR)and EILAR phase in accordance with the decrease in lung function. 2. The magnitude of EIEAR and EILAR were significantly diminished in the exercise with mask compared to that without it. 3. Both EIEAR and EILAR were inhibited by DSCG, but only EIEAR was inhibited by salbutamol. Our results suggest that chemical mediators from mast cell are very important in the occurrence of EIA, especially of EILAR.View full abstractDownload PDF (1030K) -
Tatsuya Abe, Akira IshiiArticle type: Article
1984 Volume 33 Issue 2 Pages 112-116
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe posibility of producing murine IgE with allergens of Dermatophagoides pteronyssinus (D.p.) was studied. The fraction most reactive to a pooled serum from eight mite-allergic patients (Fr.IIB) was collected from D.p. body-extract by gel-filtration on a Sephacryl S-200 column and injected to produce IgE antibody into BALB/c mice with aluminum hydroxide. The IgE-binding activities of components in Fr.IIB were measured by ELISA and PCA for human and murine sera, respectively. Sephadex G-75 column chromatography and isoelectric focusing were used to detect differences between the components bound to human and to murine IgE antibodies. The components producing the murine IgE were isoelectrically and serologically different from the allergens which were bound to human IgE. In spite of the fact that the fraction injected into the mice was allergen-rich, no murine IgE antibody against the allergens was detected in the serum.View full abstractDownload PDF (607K) -
Kazuaki YokoyamaArticle type: Article
1984 Volume 33 Issue 2 Pages 117-126
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSMethods for the detection of circulating immune complexes (CIC) using Clq EIA and anti-C3 EIA were developed. In this assay system, purified human Clq or F(ab')_2 anti-C3 antibody coated polystyrene microtiter plates were used as solid-phase and HRPO-conjugated anti-human IgG as a marker. Both techniques are simple to perform and give good sensitivity detecting artificial immune complexes (heat aggregated human IgG). One hundred twenty-two (122) patients with glomerulonephritis were studied for the presence of CIC by both methods. The incidence of CIC was the highest in lupus nephritis sera and relatively higher in sera of patients with Schoenlein-Henoch's purpura, IgA nephropathy and acute glomerulonephritis. Furthermore, it was possible to suggest the molecular size of CIC after fractionation of the sera on a Sephacryl S-300 column. The lupus nephritis sera showed several peaks with IC-activity in the various range in Clq EIA and in the small to middle size range in a-C3 EIA.View full abstractDownload PDF (1005K) -
Article type: Appendix
1984 Volume 33 Issue 2 Pages 127-
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
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Article type: Cover
1984 Volume 33 Issue 2 Pages Cover14-
Published: February 28, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (167K)
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