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Volume 33, Issue 4
Displaying 1-14 of 14 articles from this issue
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Article type: Cover
1984 Volume 33 Issue 4 Pages Cover18-
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (367K) -
Article type: Cover
1984 Volume 33 Issue 4 Pages Cover19-
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (367K) -
Article type: Bibliography
1984 Volume 33 Issue 4 Pages Misc7-
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
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Article type: Appendix
1984 Volume 33 Issue 4 Pages App3-
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
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Ken-ichi Tanaka, Yutaka Okamoto, Akemi Takeoka, Takamitsu Ino, Shigeka ...Article type: Article
1984 Volume 33 Issue 4 Pages 199-206
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSWe have found that nasal allergy can be induced in the same animal model as was developed by us for the induction of asthma. A 10% solution of TDI dissolved in ethyl acetate was painted onto the bilateral nasal vestibules of guinea pigs once daily for five consecutive days. After three weeks without treatment, challenge was made with a 5% TDI solution in the same way. This provocation procedure was repeated thereafter twice a week for about three months. During the provocation period sneezing and rhinorrhea were frequently observed in the animals, with and without dyspnea. Innumerable eosinophils were found in nasal smears. Histological survey revealed such findings as enhancement of secretory functions, eosinophilic infiltration, and probable degranulation of mast cells in the nasal mucosa. A significant increase in histamine release from the nasal mucosa was found in the TDI-sensitized animals when it was stimulated in vitro by TDI-conjugated albumin. The experimental results suggested that some common factor might exist in the pathogenesis of asthma and nasal allergy and that the animal model for asthma induced by TDI might be suitable also for basic and clinical studies of nasal allergy.View full abstractDownload PDF (1757K) -
Yukie Niwa, Ichiro Yanagida, Kyoichi SomiyaArticle type: Article
1984 Volume 33 Issue 4 Pages 207-215
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSRecently, there has been critics that assessment of phospholipid transmethylation of microsomal fraction does not necessarily represent membrane fluidity or dynamics. With out improved method, the authors assessed the methylation of membrane in human neutrophils and lymphocytes. The methyl assay was also performed with provocation by various kinds of stimulations, including opsonized zymosan, PHA, Con A and PWM. Time course experiments were also conducted. Each of cells obtained by Ficoll-Hypaque density centrifugation from heparinized venous blood was treated by ultrasound sonification for 10 seconds at 24 watts. Thereafter, microsomal fractions, including phospholipids suspended in 0.25M sucrose, were obtained by ultra centrifugation. Two μCi S-adenly-L-[methyl ^3H] methionine ([^3H]-SAM) was added to react with microsomal fractions containing about 200μg protein in each tube. Without any stimulation, phospholipids were methylated to 0.40 ± 0.050 pmol/min/mg protein [^3H]-SAM in the case of neutrophil membrane and 0.74 ± 0.075 pmol/min/mg protein in the case of lymphocyte membrane. When each cell membrane phospholipid was, 10 minutes before addition of [^3H]-SAM, stimulated with various stimulants, including opsonized zymosan and Con A, transmethylation was 20-40% enhanced with optimal concentrations. These optimal dosages of the stimulants were 1/5-1/10 the amount of each stimulant used in provocation of lysosomal enzyme release or active oxygen generation by neutrophils and of blastogenesis by lymphocytes. Time course experiments revealed that in using any stimulant, stimulation time of 10 minutes with the agent was optimal time. Analysis of methylated phospholipids performed simultaneously with transmethylation assay showed remarkably increased production of phosphatidyl choline (PC) and lyso PC concurrent with a slight increase in phosphatidyl monomethyl and dimethyl ethanolamine. Addition of methyl inhibitor (3-deaza-SIBA) inhibited the above-described transmethylation by 50-60%. These findings indicate that our phospholipid transmethylation assay actually represents the reaction of membrane phospholipids.View full abstractDownload PDF (1161K) -
Masayuki SuzukiArticle type: Article
1984 Volume 33 Issue 4 Pages 216-223
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSAntibody-dependent cell-mediated cytotoxic (ADCC) activity of peripheral blood lymphocytes (K-cells) was investigated by microplate method in 42 patients with bronchial asthma, ranging from 3 to 16 years of age, and in 12 patients with atopic dermatitis, ranging from 1 to 17 years of age. The correlation between K-cell population and each of the following factors was statistically examined; severity of disease, serum IgE levels measured by paper radioimmunosorbent test, specific IgE antibodies measured by radioallergosorbent test, and T-lymphocyte subset counted by monoclonal antibodies (OK series) using Spectrum III. The results obtained were as follows: 1) Although the K-Cell population in asthmatic children without attack was normal, the population in those with attack was increased. No significant difference in K-cell population between mild and moderate asthmatic attack was observed. The K-cell population in asthmatic children with mild atopic dermatitis was slightly reduced as compared to those without atopic dermatitis. 2) The K-cell population was extremely reduced in patients with moderate and severe atopic dermatitis. 3) There was no correlation among K-cell population, serum IgE levels, specific IgE antibodies, the percentage of OKT4^+ cells and OKIal^+ cells. However, the K-cell population was found to correlate positively with the percentage of OKT8^+ cells in patients with atopic dermatitis. These results suggest that reduction in ADCC activity of K-cells may correlate the severity of atopic dermatitis but not of bronchial asthma.View full abstractDownload PDF (815K) -
Masako KojimaArticle type: Article
1984 Volume 33 Issue 4 Pages 224-230
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSFive patients who produced erythema and wheal upon exposure to sunlight were investigated photobiologically and allergologically. The patients, but not control subjects, reacted with wheal and flare to the injection of serum or plasma taken from the patients themselves and from normal controls irradiated by projector light, indicating that the patients were allergic to plasma components of their own and normal controls modified by irradiation. Sephadex G-200 gel filtration of the irradiated plasma in 3 cases indicated that the photogenic antigens resided within molecular weight ranges of 500,000 to 1000,000 and 10,000 to 50,000 daltons in one case and 10,000 to 50,000 daltons in two cases. The photogenic antigen of another case resided within molecular weight ranges of 100,000 to 1,000,000 daltons as determined by Sephadex G-75 gel filtration. Passive transfer of solar urticaria was successfully carried our in 3 of the 5 cases by injecting patients' serum into the skin of control subjects and radiating the injection sites at times varying from 2 to 72 hours depending on cases. Heat treatment at 56℃ for 4 hours or 2-mercaptoethanol treatment completely abolished skin-sensitizing activity in one case but only weakened it slightly in the other 2 cases. Attempts to determine molecular weight of skin-sensitizing antibody by gel filtration and of immunoglobulin class by immunoadsorption were unsuccessful because of non-specific erythema appeared at skin test sites.View full abstractDownload PDF (955K) -
Taisaku Katsura, Naoki Hayashi, Shigeo Sano, [in Japanese], Tatsuhiro ...Article type: Article
1984 Volume 33 Issue 4 Pages 231-241
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe object of this study is to facilitate the detection of cases of bronchial asthma aggravated by psychological factors. The patients subjected to the study was 254 cases in age groups ranging from 15 to 80, 115 male cases and 139 female, having chronic, all year-round type, mixed-type asthma. Using a questionnaire containing 22 questions designed to revela the involvement in psychological factors in onset triggering pressence asthma, percentage scores were assigned to such categories of mental state as"extent of conditioning", "suggestibility", "expected anxiety", "dependency", "frustration", "flight into illness", "distorted life habits", "negative attitudes towards prognosis", "decreased motivation towards therapy", and"lack of understanding of mind-body relationship", and CAI-Cram curves plotted. Further, CAI-scores were derived from the CAI-Gram curves by calculating averaged of total percentages, and assessments were made on the basis of the CAI-score. Scale 1, of less than 20 points; Scale 2, of 20 to 40 points; and Scale 3, of more than 40 points. According to this scoring system, the answeres to the questionnaire are considered to reveal such that patients making a score of less than 20 points are considered normal or slightly positive (symple) for psychosomatic disease; those making a score of 20 to 40 points, positive for psychosomatic disease or neurosis; and those making a score of more than 40 points, positive for neurosis (including depression). Patients with dependency, negative attitudes towards prognosis or decreased motivation towards therapy made relatively high scores, and the patients who fell into one or both of the latter two categories of mental state were found to be experienced considerable depression. The CAI which was originated by the Society of Psychosomatic Respiratory Disease, is not yet completed, but has already been in practical use for nearly 10 years. This communication is an interim report on the use of the CAI.View full abstractDownload PDF (1257K) -
Shunsei Hirohata, Tetsufumi Inoue, Sachiko Iwamoto, Shunichi Hirose, T ...Article type: Article
1984 Volume 33 Issue 4 Pages 242-246
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSThe values of IgG, IgA and IgM in cerebrospinal fluid (CSF) obtained from four groups of patients with various neurological diseases were determined by a solid-phase enzymeimmunoassay developed by us. We have investigated the cause of the elevation of the values of these three classes of immunoglobulins in each of the four groups through statistical analysis: 1) In orthopedic disease, the elevation of the values of CSF immunoglobulins seemed to be caused by absorption disturbance of the CSF due to a block in the subarachnoid space. 2) In infectious diseases, both intrathecal synthesis and transudation of immunoglobulins from the serum might have influenced the values. 3) In idiopathic polyneuritis, the principal cause of the increase of the immunoglobulins seemed to be transudation. 4) In demyelinating diseases, local synthesis, especially of IgG, might have contributed to the abnormality of the CSF. Furthermore, IgG, IgA and IgM were not always found to increase or decrease together. Thus, the importance of simultaneous quantitation of IgA and IgM, as well as IgG, for the diagnosis of various neurological diseases was suggested.View full abstractDownload PDF (607K) -
Kazuharu Tukioka, Shigeru Hirono, Kazumitsu IshikawaArticle type: Article
1984 Volume 33 Issue 4 Pages 247-250
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSA case of occupational grape pollinosis in a 43 year old male was reported. This case seems to be the first case of grape pollinosis in Japan. The patient has been suffering from allergic rhinitis and allergic conjunctivitis during grape pollen seasons since he was 36 years old. He has been engaged in growing grapes for 20 years. Beginning 7 years ago, whenever he entered a vineyard or a vinyl plastic hothouse in which grapes were being grown, symptoms were elicited within a few minutes, but spontaneous remission occurred when he was away from the vineyard or hothouse. The results of intradermal test and PK test using grape pollen extract were positive. Nasal and conjunctival provocation tests using a minute amount of grape pollen were also positive.View full abstractDownload PDF (481K) -
[in Japanese]Article type: Article
1984 Volume 33 Issue 4 Pages 252-253
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
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Article type: Appendix
1984 Volume 33 Issue 4 Pages 254-257
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
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Article type: Cover
1984 Volume 33 Issue 4 Pages Cover20-
Published: April 30, 1984
Released on J-STAGE: February 10, 2017
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