Japanese Journal of Allergology

[title in Japanese]

Article type: Cover
1991 Volume 40 Issue 12 Pages Cover10-
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.Cover10

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[title in Japanese]

Article type: Cover
1991 Volume 40 Issue 12 Pages Cover11-
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.Cover11

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1991 Volume 40 Issue 12 Pages 1439-1446
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1439

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A CLINICAL INVESTIGATION OF THE ROLE OF ALLERGIC FACTORS IN THE SEVERITY OF BRONCHIAL ASTHMA

Sumio Kawakatsu, Atsuhiko Sato

Article type: Article
1991 Volume 40 Issue 12 Pages 1447-1453
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1447

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In order to study whether allergic factors have role in the severity of asthmatic attack, two hundred and forty adult asthmatics were clinically investigated (142 male, 98 female, aged 36.5±14.3). Fifty-seven extrinsic asthmatics were selected from the adult asthmatic group. The relationship between the total serum IgE levels, RAST scores, intracutaneous reactions to the twenty antigens, and the severity of bronchial asthma categorized according to the guidelines of the Japanese Society of Allergology, and methacholine bronchial responsiveness (Dmin(u)) were determined. In extrinstic asthma, there was a significant correlation between the severity of bronchial asthma, the intensity of asthmatic attack and bronchial responsiveness. However, there was no significant correlation between the severity of asthma, the Dmin(u) values and the total serum IgE levels, RAST scores, and the intensity of intracutaneous reaction, although there was a significant correlation between the Dmin(u) values and the numbers of positive intracutaneous reactions. In conclusion, it is suggested that there is no direct correlation between allergic factors and the severity of bronchial asthma in adult asthmatics.

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DEVELOPMENT OF HEN'S EGG WITH LOW ANTIGENICITY FOR ALLERGIC PATIENTS

Michie Hisatomi, Mamoru Kimura, Susumu Inukai, Kazuo Oshida, Akihiro M ...

Article type: Article
1991 Volume 40 Issue 12 Pages 1454-1463
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1454

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We tried to prepare hen's egg with low antigenicity for allergy patients. First, we examined the effects of pH, heating temperature and time on antigenicity of egg. Dried whole egg solid (DES) was dissolved in the buffer solution adjusted at various pH and heated at 120℃ for 10, 20 and 40 min. As a result the antigenicity didn't significantly decrease. Next, DES was treated with succinic anhydride, sodium hydroxide and was heated. The antigenicity of the resultant modified whole egg solid (MES) was much lower than that of heated whole egg solid. Antigenicity was measured precisely by enzyme immunoassay, inhibition ELISA, RAST inhibition and PCA. It was found that the antigenicity of MES was decreased less than 1/1000 of that of DES. Then SDS-polyacryl-amide gel electrophoresis and gel filtration were performed to clarify the degree of the change of egg proteins. The bands and peaks which corresponded to ovalbumin and ovomucoid known to be major allergenic proteins responsible for egg allergy disappeared and new bands and peaks were detected.

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HALOTHANE OR ENFLURANE TREATMENT IN LIFE-THREATENING ASTHMA

Kazuhiko Oki, Hironobu Sugita, Hiroyasu Takeyama

Article type: Article
1991 Volume 40 Issue 12 Pages 1464-1469
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1464

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We performed inhalation anesthetic therapy in an attempt to produce improvement in cause of life-threatening asthma, which were standard pharmacological therapy. We analysed the results obtained in 6 cases given inhalation anesthetic therapy (4 cases were treated with halothane and 2 cases with enflurane). The following observation were made: 1) The criteria for starting inhalation anesthetic therapy were persistent hypooxycemia or hypercapnia, persistently high inspiratory intra-airway pressure, clinical exhaustion and bronchial toilet with bronchofiberscope. 2) We treated the patients with halothane concentrations of between 1.0 and 2.0% and enflurane concentrations of between 1.0 to 4.2%. 3) No major complications were observed in inhalation anesthetic therapy.

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THE EFFECT OF AN ORAL GOLD COMPOUND, AURANOFIN, ON BRONCHIAL RESPONSIVENESS TO INHALED METHACHOLINE IN WELL-CONTROLLED BRONCHIAL ASTHMA

Masaaki Honma, Gen Tamura, Yukihiko Taniguchi, Tamotsu Takishima

Article type: Article
1991 Volume 40 Issue 12 Pages 1470-1476
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1470

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Recently, in Japan, auranofin (6 mg/day) has been demonstrated to be a useful treatment for patients with moderate to severe asthma in a double-blind clinical trial. Therefore, to investigate the mechanism of auranofin on bronchial asthma, we examined pulmonary functions and bronchial responsiveness to inhaled methacholine in well-controlled asthmatics after 12 wks of treatment with auranofin, in a double-blind, placebo-controlled fashion. Twenty-five adult patients with asthma received orally 3 mg of auranofin or inactive placebo twice daily for 12 weeks. Bronchial responsiveness, pulmonary function tests and concentrations of gold in the blood were measured before and 6 and 12 wks after the therapy. Bronchial responsiveness (PD_<35>-Grs) was significantly decreased after 12 wks of treatment with auranofin, compared with that 12 wks after treatment with inactive placebo. We suggest that inhibition of bronchial hyperresponsiveness by auranofin is one of the mechanisms by which auranofin is effective against bronchial asthma.

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THE DIAGNOSTIC VALUE OF A NEW WHOLE BLOOD HISTAMINE RELEASE TEST USING PAPER DISC-COUPLED ANTIGENS : Results Compared with Intracutaneous Test, RAST, and Eye Test

Kenji Tadokoro, Haruhisa Mita, Kazuo Akiyama, Tetsuo Hayakawa, Maki Ha ...

Article type: Article
1991 Volume 40 Issue 12 Pages 1477-1484
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1477

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We have developed a novel histamine release test (HR) using whole blood and antigen-coupled RAST paper discs, for the screening of allergens rather in a short time with a small amount of blood. Histamine was determined by a RIA kit. In order to evaluate the diagnostic value, the results of the test were compared with those of RAST, intracutaneous tests, and eye tests in 45 allergic patients. HR correlates better with RAST than intracutaneous test in almost all antigens. The closest positive correlation with the other tests was seen in mite allergen, followed by pollen, foods and mould spores in that order. When the HR was positive for house dust, 100% of the patients were also positive for the eye test, which is reported to closely correlate with bronchial provocation tests. HR seemed to be a useful test not only for the screening but also for the determination of pathogenic allergens.

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THE INVOLVEMENT OF RICE PROTEIN 16KD IN CROSS-ALLERGENICITY BETWEEN ANTIGENS IN RICE, WHEAT, CORN, JAPANESE MILLET, ITALIAN MILLET

Kazue Yamada, Atsuo Urisu, Hidekatsu Komada, Yoshiaki Inagaki, Masanor ...

Article type: Article
1991 Volume 40 Issue 12 Pages 1485-1492
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1485

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Cross-reactivity between rice, wheat, corn, Japanese millet and Italian millet in Poaceae family were studied by absorption test, radioallergosorbent test (RAST), and RAST inhibition assay. In absorption test using Phadebas RAST discs, more than 50% absorption of IgE antibodies was observed between rice, wheat and corn. There were significant correlations of RAST values for cereal grains including rice, wheat, corn, Japanese millet and Italian millet. RAST inhibition assay between every combinations of these cereals showed dose-dependent decrease in IgE-binding. These data indicated cross-reactivity with IgE antibodies between the five cereals in Poaceae family. RAST values for RP16KD significantly correlated with those for Italian millet as well as rice but not with those for corn and wheat. There was a trend of positive correlation between RAST values for RP16KD and Japanese millet. In RAST inhibition assay using sera with positive RAST for these five cereal grains and RP16KD, RP16KD inhibited IgE-binding to these all cereal discs in dose-dependent manner. Similarly, all of five cereal grain extracts showed an effective diminution in IgE-binding to RP16KD disc. These results indicated possible participation of IgE-binding structure on RP16KD in cross-reactivity between these cereal grains in Poaceae family.

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RELATIONSHIP BETWEEN IMMEDIATE HYPERSENSITIVE REACTIONS BY BUCKWHEAT INGESTION AND SPECIFIC IgE FOR RICE IN SUBJECT WITH POSITIVE IgE-RAST FOR BUCKWHEAT

Eiko Wada, Atsuo Urisu, Yasuto Kondo, Fumiya Horiba, Mitsutoshi Tsurut ...

Article type: Article
1991 Volume 40 Issue 12 Pages 1493-1499
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1493

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IgE-mediated mechanisms are important in immediate hypersensitive reactions (IHR) to buckwheat. However, a part of subjects with high IgE for buckwheat show no IHR to buckwheat ingestion. Inspite of cross-allergenicity between buckwheat and rice, rice ingestion rarely induces IHR even in subjects with high IgE for rice unlike buckwheat-induced IHR. We speculated that there were some relationships between the presence of IHR to buckwheat and recognition of cross-allergenic determinants on buckwheat components with rice components. We examined IgE-RAST for rice in 58 subjects with positive IgE-RAST for buckwheat. IgE-RAST for Dermatophagoides pteronyssinus (Dp), egg white and cow's milk as unrelated antigens with rice were also assessed for a comparison. Subjects (n=33) without IHR to buckwheat showed higher IgE-RAST values for rice than those (n=25) with IHR, whereas there were no differences in IgE-RAST values for Dp, egg white and cow's milk between two groups with and without IHR. IgE-RAST values for buckwheat showed significant close correlations to those for rice in subjects without IHR to buckwheat but not in those with IHR. There were no significant correlations between IgE-RAST values for buckwheat and for Dp, egg white or cow's milk in both groups with and without IHR. These results suggested that the IgE from subjects without IHR to buckwheat recognized cross-allergenic determinants with rice on the buckwheat components.

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ANALYSIS OF CLINICAL FACTORS AND IgE-RAST OF DERMATOPHAGOIDES FARINAE AND RICE IN ATOPIC DERMATITIS BY MULTIPLE FACTOR ANALYSIS OF QUANTIFICATION THEORY TYPE II

Kanata Miyakawa

Article type: Article
1991 Volume 40 Issue 12 Pages 1500-1510
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1500

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Twelve clinical factors and IgE-RAST of Dermatophagoides farinae (DF) and rice were analysed in three hundred patients with atopic dermatitis by multiple factor analysis of quantification theory Type II. Atopic dermatitis was simply defined here, as a patient who had typical eczematous eruption on the flexural portions of the body, such as the cubital fossa and/or the tibial fossa and/or the neck. The results were as follows: 1) Five clinical items such as onset age, age, history of asthma, sex, and history of allergic rhinitis were selected as significant variables to discriminate the objective variable of DF RAST, and influenced the discrimination in the order. The category of "less than 10 years-old" in the item of onset age, mostly influenced on the positive DF RAST, followed by the categories of "more than 20 years-old" in age, "presence of asthma", "presence of allegic rhinitis", and so on, in the order. 2) Five clinical items such as duration of the disease, eruption on the face, eruption on the trunks and/or extremities, sex, and age were selected as significant variables to discriminate the objective variable of rice RAST, and influenced the discrimination in the order. The category of "more than 5 years" in the item of duration of the disease, mostly influenced on the positive rice RAST, followed by the categories of refractory signs such as "lichenification on the face", "lichenification on the trunks and/or extremities", and so on, in the order. Based on the results by the multiple factor analysis, rice antigen was strongly suspected to have relation with refractory patients with atopic dermatitis.

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BIOCHEMICAL STUDY ON ANTI-INFLAMMATORY ACTION OF ANTI-ALLERGIC DRUGS : With Regard to NADPH Oxidase

Shigenobu Umeki

Article type: Article
1991 Volume 40 Issue 12 Pages 1511-1520
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1511

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The effects of anti-allergic drugs with or without H_1-receptor antagonism on the NADPH oxidase from human neutrophils in both whole-cell and fully soluble (cell-free) systems were investigated. Three anti-allergic drugs with H_1-receptor antagonism, azelastine, ketotifen and oxatomide, were found to inhibit the superoxide generation of human neutrophils exposed to phorbol myristate acetate in a whole-cell system and the activation of superoxide-generating NADPH oxidase by sodium dodecyl sulfate in a cell-free system. The concentrations of three drugs required for 50% inhibition of the oxidase (IC_<50>) were as follows: azelastine-0.7μM in the whole-cell system and 0.5μM in the cell-free system, ketotifen-60μM in the whole-cell system and 6.8μM in the cell-free system, and oxatomide-25μM in the whole-cell system and 9.7μM in the cell-free system. In addition, in the cell-free system, these drugs did not change the Km values for the NADPH of the oxidase. However, micromoles of tranilast, an anti-allergic drug without H_1-receptor antagonism, did not inhibit neutrophil NADPH oxidase in the wholeμcell and cell-free systems. The IC_<50> of hydrocortisone in the cell-free system was 60μM. These results suggest that anti-allergic drugs with H_1-receptor antagonism inhibit reconstitution of the solubilized membrane-bound enzyme by sodium dodecyl sulfate in cell-free systems and that they have a strong anti-inflammatory action. Anti-allergic drugs are not the drugs of first choice for asthma, but these drugs, especially basic anti-allergic drugs, should be used more frequently for the treatment of chronic asthma, infectious-typed asthma and mixed-typed asthma closely associated with acute and chronic inflammation of the airways as well as atopic asthma.

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HYPODENSE EOSINOPHILS ARE MUCH LABELED WITH ^<32>P THAN NORMODENSE EOSINOPHILS

Yuji Shimizu, Motohiro Kurosawa

Article type: Article
1991 Volume 40 Issue 12 Pages 1521-1524
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1521

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Peripheral blood eosinophils from the patients with atopic dermatitis were isolated on a Percoll gradient and incubated with H_3^<32>PO_4. After stopping the reaction, SDSTAG electrophoresis was performed and autoradiographs were prepared to determine the incorporation of ^<32>P into proteins. Eosinophils developed at least 14 protein bands below 66.2 K by SDS/PAG electrophoresis and the differences of the staining patterns between hypodense and normodense eosinophils were observed. In the autoradiographs 5 distinct radioactive bands were observed below 31 K. ^<32>P incorporation into the bands of hypodense eosinophils were stronger than that of normodense eosinophils, suggesting possible involvement of protein phosphorylation in the activation process of eosinophils.

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[title in Japanese]

Article type: Appendix
1991 Volume 40 Issue 12 Pages 1525-
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1525_1

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[title in Japanese]

Article type: Appendix
1991 Volume 40 Issue 12 Pages 1525-
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1525_2

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[title in Japanese]

Article type: Index
1991 Volume 40 Issue 12 Pages 1526-1533
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1526

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[title in Japanese]

Article type: Index
1991 Volume 40 Issue 12 Pages 1534-1537
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1534

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[title in Japanese]

Article type: Index
1991 Volume 40 Issue 12 Pages 1538-1563
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1538

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[title in Japanese]

Article type: Appendix
1991 Volume 40 Issue 12 Pages 1564-1569
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.1564

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[title in Japanese]

Article type: Cover
1991 Volume 40 Issue 12 Pages Cover12-
Published: December 30, 1991
Released on J-STAGE: February 10, 2017

DOIhttps://doi.org/10.15036/arerugi.40.Cover12

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