Japanese Journal of Allergology
Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
Volume 41, Issue 12
Displaying 1-21 of 21 articles from this issue
  • Article type: Cover
    1992 Volume 41 Issue 12 Pages Cover10-
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
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  • Article type: Cover
    1992 Volume 41 Issue 12 Pages Cover11-
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    Download PDF (1024K)
  • Kazuo Mori, Shunji Asakura, Norifumi Morikawa, Tokuo Chaen, Noritake W ...
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1651-1657
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    Substance P (SP) and vasoactive intestinal peptide (VIP) concentrations in nasal secretions and plasma from patients with nasal allergy to Japanese cedar pollen and healthy volunteers were measured from Jan to Dec, 1991 using EIA established by us. Simultaneously, the numbers of airborne pollens of Japanese cedar and cypress were counted, and the relation to the SP and VIP concentrations in nasal secretions from the patients with nasal allergy to Japanese cedar pollen was studied. The mean SP concentration in nasal secretions from the patients with nasal allergy to Japanese cedar pollen in the pollination season was 81.9±48.4 fmol/mg protein, which was significantly higher than that in the non-pollination season (30.4±14.7 fmol/mg protein) (p<0.01). Likewise, the mean VIP concentration in nasal secretions from the patients with nasal allergy to Japanese cedar pollen in the pollination season was 14.2±10.4 fmol/ml protein, which was significantly higher than that in the non-pollination season (4.2±3.0 fmol/mg protein) (p<0.01). The SP and VIP concentrations in nasal secretions from the healthy volunteers were not affected by the scattering of pollens. The SP and VIP concentrations in plasma from the patients and the healthy volunteers were not affected by the scattering of pollens.
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  • Akira Ishiguro, Toshikazu Shimbo, Hiromi Takahashi, Keitaro Fukushima, ...
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1658-1663
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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    Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine which can stimulate the proliferation and functions of eosinophils and neutrophils. We have studied whether peripheral blood mononuclear cells (PBMC), obtained from children with stable asthma, can produce GM-CSF through stimulation with crude Dermatophagoides farinae (Df) antigen in vitro. The patients had strongly positive RAST results for house dust mite Df. Levels of GM-CSF in PBMC culture supernatant were assayed by an enzyme immunoassay. When stimulated with Df antigen, patient's PBMC released significantly increased levels of GM-CSF compared with unstimulated ones. PBMC of non-allergic controls, however, did not. The addition of polymyxin B, an inactivator of endotoxin, did not suppress the Df-antigen-induced production of GM-CSF. Levels of GM-CSF produced by PBMC stimulated with Df antigens were significantly higher in the patients than in the controls. These results indicate that PBMC of children with Df-allergic asthma, unlike those of non-allergic children, can produce elevated levels of GM-CSF by stimulation with Df antigens.
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  • Koji Kawaji, Yoichi Nakamura, Toshio Ozaki, Toshihiko Kamei, Kayo Bann ...
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1664-1671
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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    The in vitro production of granulocyte/macrophage colony stimulating factor (GM-CSF) by mononuclear cells from the peripheral blood of patients with bronchial asthma (BA) was examined by enzyme-linked immunosorbent assay (ELISA). In 3 of 12 cases studied, mononuclear cells from BA patients produced GM-CSF without stimulation. And in 5 of 12 cases studied, mononuclear cells from BA patients produced GM-CSF in response to IL-2. Mononuclear cells from patients with other diseases (n=13) and healthy voluneteers (n=6) did not release any detectable (≧7.5 pg/ml) GM-CSF. The culture media of mononuclear cells from BA patients showed activities for stimulating the proliferation and survival of eosinophils, and these activities were partially inhibited by anti-GM-CSF antibodies. GM-CSF production by mononuclear cells from BA patients treated with prednisolone was lower than that of mononuclear cells from untreated BA patients. And prednisolone showed a reduction in the GM-CSF production from mononuclear cells in response to IL-2. These results suggest that GM-CSF production by mononuclear cells may play a role in the pathogenesis of BA.
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  • Mari Miyoshi, Takashi Sakurai, Soichi Kodama
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1672-1678
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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    Serum ECP levels, serum MBP levels and blood eosinophil counts (Eo) were studied in patients prepared for asthma attack. Five severely asthmatic children were studied. Samples were taken just before their 33 times of home stay loading test. Serum ECP levels were significantly higher in the patients who suffered asthma attacks during their home-stay period than in those who did not. In addition, serum ECP levels were higher in the patients whose asthma attack started less than 12 hours after they returned home. However, there was no significant correlation either in serum MBP levels or peripheral eosinophil counts between these groups. These findings suggested that serum ECP levels increased in patients prepared for asthma attack.
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  • Kyoko Matsumoto
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1679-1686
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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    To evaluate the clinical features of respiratory syncytical virus (RSV) infection and relation with bronchial asthma, 233 infants hospitalized for lower respiratory tract infection during winters from 1988 to 1990 were enrolled in this study. RSV antigen was detected from half of them by immunofluorescent antibody test. Children with RSV infection showed the characteristic findings as follows; wheeze (71.6%), elevated serum 2-5 AS activity, a marker for viral infection, low level of acute phase reactants. Patients whose family members with major allergy experienced more frequent episodes of wheeze. Since the initial RSV infection could cause wheeze among infants from atopic families and recurrent wheezy infants could be recognized as in the pre-stage of infantile asthma, we should follow these infants with RSV infection closely.
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  • Yuji Maeda, Kazuo Akiyama, Tetsuo Hayakawa, Maki Hasegawa, Fujito Kane ...
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1687-1693
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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    Beclomethasone dipropionate (BDP) administered by inhaler is a very useful drug for the treatment of bronchial asthma. In this therapy, it is very important to use steroids systematically to induce a complete remission of asthma attack (first step) and then begin to use BDP a dose of more than 800 μg to maintain remission (second step). We treated 27 difficult asthmatics with this therapy and found this new method very useful. The characteristics of asthmatics were as follows. 1) The age ranged from 37 to 82, and the mean age (±S.E.) was 58.9 (±2.6) years old.2) The onset age ranged from 27 to 74 with a mean age (±S.E.) of 46.9 (±2.6) year old. 3) The number of non-atopy was 22 cases. 4) The follow-up duration ranged from 5 to 45 months with a mean (±S.E.) of 15 (±2.0) months. The results were as follows. 1) The complete remission rate was 48%, partial remission 37% and unchanged 15%. 2) There was a significant increase only in %VC. 3) The peripheral eosinophil count was decreased significantly. 4) The log value of PC_<20> concentration by acetylcholine increased significantly by a factor of 2.94 to 3.28. 5) After this therapy, the mean serum cortisol level at 9:00 a.m. was 10.1 (±3.8, S.E.) μg/ml. There were only 2 cases whose cortisol level were under the normal. 6) There were many oral side effects, namely stomatitis with 5 cases and hoarseness with 8.
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  • Takemasa Nakagawa, Yuji Okano, Eisaku Iwasaki, Kenichi Akimoto, Toshik ...
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1694-1704
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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    The measurement of IgE and IgG4 antibodies against egg white, milk, soybean and Dermatophagoides farinae was performed by FAST (fluorescence allergosorbent test) using 21 serum samples obtained from non-allergic children and 160 serum samples from atopic children with bronchial asthma and/or atopic dermatitis. Their antibody levels were evaluated for any association with disease severity and for clinical significance in estblishing diagnosis. It was found that children with bronchial asthma showed lower levels of IgE antibodies against egg white, milk and soybean and higher levels of IgE antibodies against Dermatophagoides farinae compared with those of children with atopic dermatitis, while both groups showed higher levels of egg white and milk-specific IgG4 antibodies compared with non-allergic children. These IgE and IgG4 antibody levels revealed a tendency to correlate with disease severity in patients with atopic dermatitis, while this was not observed in patients with bronchial asthma. The contribution percentages of IgG4 antibody determination, together with IgE antibody determination, in retrieving causal allergens were 71% for egg white, 70% for milk and 48% for soybean allergy, implying their diagnostic value in establishing clinical diagnosis.
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  • Yuji Shimizu, Motohiro Kurosawa
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1705-1709
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    Peripheral blood eosinophils from normal healthy subjects were isolated on a Percoll gradient and were incubated with [^<32>P] orthophosphoric acid. ^<32>P-labeled eosinophils were washed and stimulated with GM-CSF under different conditions. After reaction was stopped, SDS/PAG electrophoresis was performed along with autoradio-graphs to determine the incorporation of ^<32>P into proteins. GM-CSF-stimulated eosinophils produced an increase of ^<32>P incorporation into the bands in the 31 kDa and 35 kDa areas after 30 sec and 1 min of stimulation. The increase of ^<32>P incorpolation was dependent on Mg^<2+> concentration and temperature, suggesting that proteins in the eosinophils with apparent molecular weights of 31 kDa and 35 kDa can be phosphorylated with the stimulation of GM-CSF.
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  • Takahiko Kumada, Hiroshi Fujimiya, Hideo Miyata, Yoshiko Banno, Yoshin ...
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1710-1716
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    We investigated the signal transduction of serotonin secretion by stimulation with DNP-Ascaris antigen or ionomycin in rat basophilic leukemia cells (RBL-2H3). The modes of action of antigen and ionomycin for serotonin secretion were shown to be similar. The treatment of cells with antigen resulted in increased tyrosine phosphorylation of 105 and 72 KDa proteins, in particular, the tyrosine phosphorylation of 72 KDa protein seemed to correlate with serotonin secretion. Furthermore, we observed that antigen stimulation caused a marked increase in inositol polyphosphates production, which derived from the tyrosine phosphorylation of phospholipase C-γ in RBL-2H3 cells. On the other hand, treatment with ionomycin also resulted in an increase in tyrosine phosphorylation of 72KDa protein, but did not induce inositol polyphosphates production. These results suggested that the activation of tyrosine kinase may be related to serotonin secretion, and that intracellular Ca^<2+> increase may also play an important role in this activation.
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  • Goro Kimura, Kazuro Ogurusu, Ryo Soda, Shinya Tada, Kiyoshi Takahashi, ...
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1717-1721
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
    JOURNAL FREE ACCESS
    T-cell-derived lymphokine, IFN-γ, has potent effects on B-cell differentiation and leukotriene C4 production in leukocytes, and inhibits the effect of IL-4 on IgE production. To investigate the role of IFN-γ in the pathogenesis of bronchial asthma, we examined IFN-γ production by peripheral blood mononuclear cells cultured with Candida antigen and serum Candida specific IgG1 antibody from 20 asthmatics. The results were as follows: 1) IFN-γ production in non-atopic severe asthmatics was significantly higher than in healthy subjects, atopic mild and moderate asthmatics, and atopic severe asthmatics (p<0.05). 2) There was a significant correlation between IFN-γ production induced by Candida antigen and serum Candida specific IgG1 antibody (r=0.72, p<0.01). These results suggest that IFN-γ may play an important role in the pathogenesis of non-atopic severe bronchial asthma.
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  • Yoshifuji Matsumoto, Shigemitsu Ninomiya
    Article type: Article
    1992 Volume 41 Issue 12 Pages 1722-1725
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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    Allergy is a common feature of patients with chronic fatigue syndrome (CFS). Because of this strong association, we attempted to explore the prevalence of allergies among Japaneses patients with CFS. Of the present 18 patients, 78% had allergies during their premorbid and/or postmorbid conditions. Their allergies were mainly cutaneous reactions including drug allergies and 43% of the patients had 2 or more allergic reactions. In the case of a premorbid condition, allergies improved spontaneously after onset of CFS. Clinical manifestations of CFS, however, became worse during the period of an association with allegies. Immunologic tests, including peripheral blood lymphocyte-subsets, blastogenesis, natural killer-cell functions and cytokine-assays, were not any correlation between both patients with and without allergies.
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  • Article type: Appendix
    1992 Volume 41 Issue 12 Pages 1726-1727
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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  • Article type: Appendix
    1992 Volume 41 Issue 12 Pages 1728-
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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  • Article type: Appendix
    1992 Volume 41 Issue 12 Pages 1729-
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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  • Article type: Index
    1992 Volume 41 Issue 12 Pages 1730-1738
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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  • Article type: Index
    1992 Volume 41 Issue 12 Pages 1739-1742
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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  • Article type: Index
    1992 Volume 41 Issue 12 Pages 1743-1772
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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  • Article type: Appendix
    1992 Volume 41 Issue 12 Pages 1773-1778
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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  • Article type: Cover
    1992 Volume 41 Issue 12 Pages Cover12-
    Published: December 30, 1992
    Released on J-STAGE: February 10, 2017
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