The histopathological study was carried out to clarify damage in the vestibular organs and cochlea in 93 guinea pigs receiving various aminoglycoside antibiotics (AGs); micronomicin (MCR), kanamycin (KM), dibekacin (DKB) and amikacin (AMK). Pinna reflex test in a wide frequency range from 20k to 500Hz was performed before, during and after the administration of AGs. Any vestibular function test was not done but histopathological investigation of the inner ear was carried out on the serial celloidin and celloidin-paraffin sections of the bilateral temporal bones. The following results were obtained.
1) The hair cell loss always occurred earlier in the vestibular organs than in the spiral organ. 2) The hair cell loss was scattered in mild vestibular lesion but extensive in the severe lesion. 3) The hair cell loss was first seen in the utricular and/or saccular maculae and later in the ampullar crests of the semicircular canals. 4) The hair cell loss occurred in some of regions in mild vestibular lesion, but in all of them in the severe lesion. 5) The bilateral occurrence of the hair cell loss in the individual regions was noticed to the same extent in more than 80%. 6) Lower dose (50, 25, 15, 10mg/kg) of the aminoglycosides, either KM, DKB and AMK with relatively high ototoxicity or MCR with low ototoxicity induced very limited loss of the outer hair cells mostly at the basal end of cochlea in low incidence (less than 20%). In the vestibular organs, hair cell loss was scattered, but occurred in almost 100% of the animals given the above mentioned drugs. 7) At high dose (100, 150, 400mg/kg), there was distinguished difference in incidence and extent of the outer hair cell loss between the aminoglycosides with high ototoxicity and MCR. KM and DKB caused extensive loss of the outer hair cells in high frequency (83, 100%), but MCR induced limited loss of the outer hair cells in low incidence. In the vestibular organ, the hair cell loss was extensive 100% in the animals treated with KM and DKB, but 100% in less extensive area in the animals treated with MCR. 8) These results may contribute to understand the affinity of the aminoglycosides for the vestibular and spial organs.
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