(1) In the present paper, further con-firmation was obtained by repeated in-tensive experiments with Co-porphyrin-derivatives freshly prepared from pure chlorophyll a and b by the column chromatographic method of Gattermann and Wieland. The effect on
Euglena(chlorophyll free) was always revealed to be positive.
(2) Moreover, vitamin B
12.-mass pro-ducing experiments with Co-porphyrin-derivative and 5, 6-dimethylbenzimidazole was attempted by
Euglena gracilis strains in B
12-Free basal medium, and vitamin B
12 was isolated from both cells and broth by paper electrophoresis. The increase of a fair quantity of total vitamin B
12 was always seen in the cells and the broth of control strains.
(3) Therefore, the authors are inclined to propose the close implication of cobalt-porphyrin-derivative in the biosynthesis of vitamin B
12 by
Euglena.
抄録全体を表示