1) Glyoxylate and succinate were formed in addition to pyruvate and a-ketoglutarate, when the cell free extracts of
coli-aerogenes grown on either glucose or citrate were incubated in the presence of citrate.
2) The fresh cell extracts of
coli-aerogenes revealed a very poor potency of producing
α-ketoglutarate with citrate, whereas, the addition of coenzyme II (TPN) to the reaction mixtures resulted in an increase in formation of
α-ketoglutarate from citrate.
3) It was found with
coli-aerogenes that iso-citritase existed in the various cells obtained from the following growth- media: bouillon-, peptone-, glucose-, acetate plus bouillon- suc-cinate plus bouillon- and citrate plus bouillon (Peptone)- media.
4) Isocitritase was demonstrable, even when
coli-aerogenes was anaerobieally grown on a citrate-bouillon medium.
5) A large amount of glyoxylate was excreted in the external media, when not only the dried and ground cells of
coli-aerogenes, but the intact (washed) cells of
A. aerogenes were also incubated in the presence of citrate and a trapping agent such as semicarbazide.
6) Isocitric dehydrogenase existed in the cells of
coli-aerogenes. 7) From the results obtained in the previous and present papers, it was strongly suggested that the intracellular concentration of coenzyme II of
coli-aerogenes might be relatively low, so that coenzyme II-dependent isocitric dehydro-genase could not be operative at such a high rate sufficient to effect the complete removal of citrate.
8) It was indicated that the major degrada-tion of C
6-triearboxylie acids by
coli-aerogenes took place by way of splitting reactions rather than depending on the isocitric-a-ketoglutaric dehydrogenase system.
9) With both C
3- and C
4-acids,
α-ketoglutaratc was again ascertained to be obtainable in a high yield.
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