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Part IV. On the Movement of Cecal Contents in the Rabbit (1)
Ichiro YOSHIHARA, Makoto KANDATSU
1960 年 24 巻 6 号 p.
543-547
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
For the study of cecum digestion, the movement of cecal contents in the rabbit with abdominal window was studied by injecting
35S-barium sulfate into the cecum. The fecal output and the distribution of radioactivity in cecum, indicated that the hard and soft feces in the rabbit are formed from the same cecal contents. A new assumption was thus suggested to explain the formation of the hard and soft feces.
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Yoshiro KUROIWA, Naoki HASHIMOTO
1960 年 24 巻 6 号 p.
547-554
発行日: 1960年
公開日: 2008/11/27
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1) In the case of yeast, it has been demon-strated that acid-soluble compounds are not sufficiently eliminated when extracted twice with 0.2 N PCA according to Ogur and Rosen's method.
2) Even if the extraction with 0.2 N PCA is repeated further, acid-soluble compounds are not extracted completely; on the contrary, RNA begins to dissolve into the acid-soluble fraction.
3) It has been shown that these defects can be eliminated only when acid-soluble com-pounds are extracted with water instead of with 0.2 N PCA.
4) Based on the facts described above, the previous report on the determination of nucleic acids in fresh or durable yeast has been further modified, from the viewpoint of elution behavior of acid-soluble compounds.
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Part VI. Basic Properties of Each Crystalline Component
Hideo CHIBA, Etsuro SUGIMOTO, Makoto KITO
1960 年 24 巻 6 号 p.
555-557
発行日: 1960年
公開日: 2008/11/27
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The apparent Michaelis constants of each component for the coenzyme are the same values.
Each component is inhibited by the substrate according to the same mechanism.
The optimal pH values and the equilibrium constants of the reactions catalyzed by each component are the same.
E
1%1cm (280mμ) values of each component are are identical. E
280mμ/E
260mμ absorption ratios also similar.
It may be concluded that each component is almost the same enzyme protein.
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Part VII. Crystalline Preparations from Autolysates Incubated for Various Periods
Hideo CHIBA, Etsuro SUGIMOTO, Makoto KITO
1960 年 24 巻 6 号 p.
558-561
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
The relation between the enzyme activity of the watar extract and the autolysis time is investigated.
The crystalline PGA mutase preparations having the same crystalline forms and different specific activities are obtained from the auto-lysates incubated for different periods at 38°C.
The specific activities of these crystals decrease as the autolysis time is prolonged.
The content of component I in these crystals decreases in proportion to the autolysis time.
The 20 hr.-crystal is component V only.
The PGA mutase protein is only component I in yeast cells and this component is modified by PGA mutase-modifying enzyme during autolysis.
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Part IV The Decompositions of Several Mercaptals and Mercaptoles of Cysteine in Aqueous Solutions
Yataro OBATA, Junya MIZUTANI
1960 年 24 巻 6 号 p.
562-564
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
Cysteine mercaptals and mercaptoles were prepared by the reactions of L-cysteine with formal-dehyde, acetaldehyde, n-butyraldehyde, benzaldehyde, furfural, pyruvic acid and levulinic acid in 6 N hydrochloric or sulfuric acid. Hydrogen sulfide released from cysteine mercaptals and mercaptoles in heated aqueous solutions (oil bath:120°C) was determined. Although a small amount of hydrogen sulfide was liberated from L-cystine on one hour heating, its amount increased suddenly after three hours. Among these compounds L-cysteine mercaptal of furfural was most unstable and a large amount of hydrogen sulfide was produced.
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Studies on the Reticulo-rumen Digestion. Part XVII
Masaaki HORIGUCHI, Makoto KANDATSU
1960 年 24 巻 6 号 p.
565-570
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
In the course of study on the amino acid composition of sheep rumen Protozoa, a new ninhydrin-positive substance was isolated from acid hydrolysates of a proteolipid-like material of Protozoa. This substance was ideneified as 2-aminoethane phosphonic acid, and the occurrence of a C-P bond in Nature has been demonstrated for the first time.
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Hisateru MITSUDA, Kyoden YASUMOTO, Fumio KAWAI
1960 年 24 巻 6 号 p.
570-575
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
Although the manometric study of the catalatic reaction has several drawbacks in comparison with the titrimetric method, the manometry has often been used to determine the catalatic activity of an impure preparation because the oxygen evolution from hydrogen peroxide is owing exclusively to the action of catalase.
To overcome the drawbacks a sensitive recording electronic manometer was constructed using a RCA 5734 subminiature triode, a mechano-electronic transducer, with diaphragms, which was reliable for the studies of not only the catalatic reaction but also other reactions which accompany a rapid or minute pressure variation. Measurements of the catalatic reaction with the apparatus was satisfactorily compared with the titrimetry.
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Part I. Triose Metabolism
Hideo KATAGIRI, Kazutami IMAI, Katsumi ITO
1960 年 24 巻 6 号 p.
575-580
発行日: 1960年
公開日: 2008/11/27
ジャーナル
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1.
L. fermentum can utilize glyceraldehyde, forming almost equimolar lactic acid per triose consumed. Neither the evolution of carbon dioxide nor the uptake of oxygen was observed manometrically, and any by-product such as volatile acid, ethanol or acetoin was not detected in the process of its dissimilation.
2. Glyceraldehyde-3-phosphate, 3-phospho-glycerate and pyruvate were identified as inter-mediates of triose metabolism; the two formers were equally labeled with inorganic P
32 in reac-tion systems.
3. Considerable degree of inhibition by both iodoacetate and dinitrophenol on triose dis-appearance as well as on lactic acid formation was observed. In consequence, possibility of the presence of Embden-Meyerhof route for triose utilization and some relating problems are discussed.
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Part II. Mannitol Utilization
Hideo KATAGIRI, Kazutami IMAI, Katsumi ITO
1960 年 24 巻 6 号 p.
581-587
発行日: 1960年
公開日: 2008/11/27
ジャーナル
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1. Resting cells of both homo- and heterofermentative lactic acid bacteria oxidized mannitol aerobically to produce lactic acid, where half mole of oxygen per mole of utilized mannitol was found to be consumed monometrically. But anaerobically no consumption of the substrate was observed.
2. The crude cell-free extracts of heterofermenters oxidized mannitol to fructose, accompanied by approximately theoretical yield of reducing sugars, and those of homofermenters oxidized mannitol-1-phosphate to fructose-6-phosphate in the same manner, and not
vice versa.
3. The presence of mannitol dehydrogenase in the extracts of heterofermenters, and mannitokinase and mannitol-1-phosphate dehydrogenase in the extracts of homofermenters were demonstrated spectrophotometrically. DPN-dependence of both dehydrogenase and reversibility of the reactions were ascertained.
4. Mannitol dehydrogenase was purified and about 40-fold active preparation was obtained from
Lactobacillus brevis. The enzyme is specific for mannitol and activated by cobalt ion.
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Part III. Mechanism of Ethanol Formation
Hideo KATAGIRI, Kazutami IMAI, Katsumi ITO
1960 年 24 巻 6 号 p.
588-594
発行日: 1960年
公開日: 2008/11/27
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1. Pyruvate and acetaldehyde were detected as intermediates in the pathway of heterolactic fermentation by
L. fermenti.
2. Acetaldehyde was accumulated aerobically as an oxidation product of ethanol in the pre-sence of semicarbazide.
3. Dismutation of acetaldehyde to ethanol and acetate was observed when acetaldehyde was added to the system of glucose fermenta-tion.
4. Alcohol dehydrogenase in an ammonium sulfate fraction catalyzes both DPN- and TPN-dependent oxidation of ethanol to acetaldehyde. Acetyl CoA was reduced in the presence of either TPN or DPN with the same fraction.
5. By coupling of D-xylose degradation with mannitol utilization appreciable increase of ethyl alcohol was observed with resting cells of heterolactic microorganisms.
6. With the continuous supply of reduced methyl viologen approximately half molarity of acetate to be produced from D-xylose was transformed to ethyl alcohol in an absolutely anaerobic condition. From L-arabinose only poor amount of ethyl alcohol was produced by both strains of microorganisms.
7. A metabolic pathway of lactic acid fer-mentation including D-mannitol fermentation was suggested.
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Part VIII. On the Reduction of (-)-Isomenthone
Hiroo UEDA
1960 年 24 巻 6 号 p.
595-597
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
In the reduction of (-)-isomenthone with sodium in aqueous ammonia, it was found that the reduction product gives a mixture consisting of 75.5% of (-)-isomenthol (a, e, e), 9.5% of (+)-menthol (e, e, e) and 8.6% of (-)-neoisomenthol (a, a, e). From this fact, it might be concluded that this reduction is stereospecific for isomenthone and is mechanistically different from reduction with sodium and alcohol.
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Part IX. On the Reduction of (±)-Piperitone
Hiroo UEDA
1960 年 24 巻 6 号 p.
598-600
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
In the reduction of (±)-piperitone with sodium in aqueous ammonia, by means of infrared analyses, it was found that the reduction product is composed of (±)-isomenthol in a slight excess over (±)-menthol, and the components were isolated in a pure state.
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Part X. Synthesis of Menthol from Acetone and Formaldehyde
Hiroo UEDA
1960 年 24 巻 6 号 p.
601-603
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
On the reduction of the condensation product of mesityloxide with methylvinylketone by treatment with sodium in aqueous ammonia, the main product was (±)-menthol and (±)-isomenthol, which were isolated in pure state. This experimental result suggests the possibility of menthol synthesis from acetone and formaldehyde.
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Part III. Polarography of Isoniazid and Sarkomycin-isoniazid Derivative. 1
Keiji IDA
1960 年 24 巻 6 号 p.
604-609
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
By polarography, isoniazid (INH) gives three reduction waves and sarkomycin-isoniazid derivative (SK-INH) gives three or four waves. Polarography of the mixtures of two out of sarkomycin (SK), INH and SK-INH suggests that the first, the second and the fourth wave of SK-INH are derived from the first, the second and the third wave of INH, respectively, and the third wave from SK. Alternating current polarography of SK-INH shows three or four maxima, whose numbers are the same as wave numbers in direct current polarography.
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Part IV. Polarography of Isoniazid and Sarkomycin-isoniazid Derivative. 2
Keiji IDA
1960 年 24 巻 6 号 p.
610-613
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
Quantitative analyses of sarkomycin-isoniazid derivative (SK-INH), isoniazid (INH) and their mixture are discussed. The diffusion current of SK-INH gives a linear relationship with the concentration only in the same lot, but this calibration curve cannot be applied for samples of other lots. Increase in the diffusion currents of the first and the second wave in a mixture of SK-INH and INH yields straight lines against the added INH, which coincide well with the calibration curve of INH. Relation between diffusion currents of the first and the second wave and nitrogen contents, obtained by a lot of SK-INH, cannot be used for other lots of sample. Ratios of the diffusion currents of these waves show different values in each sample, which are larger than in INH.
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Über das Vorkommen des trans-Isomeren in der natürlichen Blätteralkohol-fraktiontt
Von Akikazu HATANAKA, Minoru OHNO
1960 年 24 巻 6 号 p.
614-617
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
Es wurde aufgeklärt, daß die im Tee-blättern weit verbreitet vorkommende natürliche Blätter-alcohol-fraction aus einem Gemisch der
cis,
trans-Isomere besteht, wobei bisher das
cis-Isomere besteht, wobei bisher das
cis-Isomere stark überwiegt.
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Tatsuo KOYANAGI, Sadao MIURA
1960 年 24 巻 6 号 p.
617-621
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
The high concentration of sodiumchloride content in the diet has deleterious effects both on the occurrence of liver cancer induced by
p-dimethyl amino azobenzene through decreasing riboflavin concentration of liver and on the production of hypertension in rats. The effects can be largely counteracted by the simultaneous feeding of riboflavin.
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Part I. Isolation of strains capable of producing amino acids frompentose and their taxonomical studies
Koichi YAMADA, Yoshio HIROSE
1960 年 24 巻 6 号 p.
621-627
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
Isolation and screening tests were carried out to obtain microorganisms capable of producingamino acids from pentoses and hexoses directly in the presence of nitrogen sources and inorganicsalts.
Three strains having exceedingly high amino acids producing ability were obtained. Oneof them produces remarkably L-glutamic acid and alanine and the other two have high pro-ductivity of alanine.
As a result of the taxonomical studies, all of these strains were found to belong to the Genus
Brevibacterium. Moreover, there are no species which conform to these strains described inBergey's Manual of Determinative Bacteriology 7th Ed., so the authors decided them to be newspecies.
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Isao YAMAMOTO, Yuzuru YAMAMOTO
1960 年 24 巻 6 号 p.
628
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
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Seiji NAKAMURA
1960 年 24 巻 6 号 p.
629-630
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー
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Synthesis of Gibberellins. Part I. Infrared Spectra of the Lactones of Cyclohexane Series
Kenji MORI, Masanao MATSUI, Yusuke SUMIKI
1960 年 24 巻 6 号 p.
631-632
発行日: 1960年
公開日: 2008/11/27
ジャーナル
フリー