(1) An antineuritic preparation so-coiled “Active Oryzanin” was prepared by treating the aqueous extract of rice polishings with Japanese acid-clay and phosphotungstic acid as described above. Its antineuritic activity is found to be in the daily dose of 4mg. for pigeons as well as for white rats and the Table II. Isolation of Oryzanin (Autineuritic Vitamin.)
pigeon curative day-dose to be of the order of 0.74mg. while it lacks the pellagra protective factor and growth promoting factor.
(2) Starting from “Active Oryzanin” the author isolated the antineuritic vitamin in crystalline state according to the process of Jansen & Donath with minor modification, besides adenin, cholin, hypoxanthin, nicotinic acid, two unknown bases (C₃H₆N₂)_n & C₈H₁₀NO₃ etc.
The yield of the antineuritic crystals was very scanty, about 0.85g. of the purified hydrochloride (1.31g. of crude crystals) was isolated from 2kg. of “Active oryzanin” which were prepared from about 6.000kg. of rice polishings.
These results are summarised in the following schematic table II.
(3) The antineuritic compound isolated by the author, crystallises in colorless long monoclinic plates, melting at 250°C. It gives Pauly's diazo-reaction in quite different coloration to compare with that given by imidazol compound and it contains sulphur in the molecule in a form detectable by sodium nitro-prusside or by lead acetate when it is boiled with alkali or fused with metallic sodium previously. From the analytical results, it was ascertained that the compound should be the hydrochloride of a sulphur compound having the formula C₁₂H₁₆N₄SO₂. These principal points are not agreed with that reported by Jansen and Donath.
(4) Biological experiment on pigeons and rats showed its antineuritic activity to be in the daily dose of 0.01_??_0.02mg. From its activity, the content of antineuritic substance in rice-polishings is presumed to be about 0.00039%.
The further chemical and biological studies on this compound are expected in the next report.

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