(1) The hydrochloride of oryzanin which was prepared in pure state directly from so-called “ Oryzanin-extract” by silver-fractionation, platinum precipitation in alcohol and repeated recrystallisation from alcohol and acetone, possessed entirely the same properties with that purified further through picrolanate and chloraurate. The analytical results agree with the formula C
12H
16N
4SO
2-2HCl which was given by the author in the previous report. The yield: 1.6g. from 11.500kg. of rice-polishings.
(2) The hydrochloride cured and protected pigons as well as albinorats from polyneuritis with the daily dose of 0.005_??_0.01mg. The curative day-dose for a pigeon was found to be the order of 0.0025mg.
(3) Besides the hydrochloride, the author prepared the picrolonate (C
12H
16N
4SO
2-2C
10H
8N
4O
5 M.Pt. 226°C), the picrate (C
12H
16N
4SO
2 2C
6H
3N
3O
7 M.Pt. 208°C), the chloraurate (C
12H
16N
4SO
2-2HAuCl
4 M.Pt. 189°C) the chlor-platinate (C
12H
16N
4SO
2-H
2PtCl
6) and finally the free base (amorphous), and studied their properties.
(4) From these results, it was confirmed that the antineuritic substance isolated from rice-polishings by the author, is a new sulphur compound having the empirical formula C
12H
16N
4SO
2.
Further studies on the chemical constitution of this substance will be reported later on.
The author expresses his sincere thanks to Prof. Dr. U. Suzuki for his kind advise and encouragement throughout the work. Thanks are due to Sankyo Company, Ltd. for kind supply of the material. The author is also indebted to Messrs M. Kamada and T. Yamagishi for their assistance both in chemical and biological experiments.
(May 12 th., 1932).
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