(1) The isolation and determination of fatty acids of tunny liver oil were performed, and further the examination of the curative value of its vitamin-A was carried out.
(2) The fatty acids contents of the liver oil, collected in spring, were about as follows:
Oleic acid 30% Clupanodonic acid 22%
Arachidonic acid 20% Palmitic acid 19%
Stearic acid 7% Myristic acid 5%
Small amount of Linolic acid.
(3) The curative power of the vitamin of this oil was determined by biological test, and 9mg. of the oil per day for one albino rat was found to be sufficient.
Before closing this article, the writer wishes to express to Profs. U. Suzuki and M. Yamagawa his sincere acknowledgement and appreciation fot their kind direction given him throughout this research work.

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(1) In the above experimental result the nitrogenous compounds islated From “di-saké” are:
_??_
(2) It is the interesting fact that ths “di-saké” contains fairly amount of agmatine, which had never been isolated from any other fermentation products.

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(1) We have studied the crystalline saponin of soya bean which Y. Sumiki had investigated already, and have obtained the results that its molecular formula is C_{48_??_50}H_{77_??_81}O₁₈ and the products of hydrolysis are sapogenin (C_{20_??_32}H_{48_??_52}O₃), glucuronic acid, galactose, and rhamnose.
(2) Now we have isolated at the same time an amorphous saponin, of which molecular formula is C_{49_??_51}H_{79_??_83}O₁₉. Its sapogenin is C_{31_??_33}H_{50_??_54}O₄ and the others are identical with the former. Both have not haemolytic power.
(3) Each saponin is isolated five isomers according to the period of the hydrolysis and is required each mutual relation. And we have showed that the molecular formula of these neutral sapogenins are respectively resemble to those of the acidic sapogenins, oleanolic acid and hederagenin.
(4) If these saponins and sapogenins are heated with copper oxide or lead chromate, they liberate the considerable volume of methan or ethan gas. We have isolated the two more saponins that are water-soluble and haemolytic (crystal melts at 270° and amorphous 243°), of which we are to report later. Then we think that three kinds of saponin E. Walz showed are the following kinds of ours. One of them (mel. pt. 225°) is the crystalline saponin, ones (mel. pt. 280° hexagonal) is its natrium-salt and the remains (mel. Pt. 272° haemolytic) are the saponin that is water-soluble.

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Reducing sugar, which is scarcely detected in the seeds, is increased the earlier stage of germination and decreased afterwards. Cane sugar and stachyose are decreased gradually. Starch and dextrin are markedly increased. Galactan is decreased ; araban and crude fiber are increased. The total amounts of carbohydrates do not show any marked changes, during the development of the seedlings in the periods of the experiment.

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(1) Growth and the general condition of animals are not attained by the diets containing 76_??_78% of polished-rice powder, rice-starch, or halfpolished rice as carbohydrates, except unpolished-rice diet, though an adequate amount of alcoholic yeast extract is provided. When, however, the yeast extract is replaced by whole dried yeast there appears a prompt recovery of the abnormal condition. The most characteristic phenomena are the occurrence of bareness on the body and of bleeding around the nose, the mouth, and the fore paws.
(2) Lecithin and soy-bean oil possess an almost equal nutritive value in animal physiology to linoleic acid.
(3) Chrysalis oil induces an excellent growth in rats, owing to its content of unsaturated fatty acids, namely linoleic and linolenic acids.
(4) The abnormal condition in nutrition of the rats fed on the rice-bran oil is not improved by administration of either chrysalis oil or linoleic acid. The cause of malnutrition and of the failure of growth with the former oil is not yet clear.
(5) The results of experiments with fatty acids prepared from cod-liver oil show that they cannot maintain the growth of animals and that the large dose causes a disturbance of the gastro-intestine.
(6) Linoleic acid is unable to “spare” either vitamin B₁ or B₂.
The author wishes to express her deep gratitude to Professor U. Suzuki for his many helpful suggestions throughout this work. She is indebted to the Keimei Society for financial assistance and is also grateful to Miss K. Miti for her willing help in feeding of the animals and preparing of the diets.

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