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Volume 25, Issue 2
Displaying 1-16 of 16 articles from this issue
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Part I. On a New Antifungal Organic Mercurial, MethylmercurithioacetamideTakuro SADO, Fumio MIYAMOTO, Kuniichiro YANO, Ichiro SATO, Hideo OSE, ...1961 Volume 25 Issue 2 Pages 77-82
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSNineteen new alkylmercurithio derivatives were synthesized, and their antibacterial and antifungal activities were studied in comparison with Thimerosal, a representative commercial mercurial disinfectant. All the substances tested showed stronger activities than the latter agent. Among them, methylmercurithioacetamide was recognized as the most suitable remedy for dermatomycosis because of its high solubility and stability in alcoholic solvents for tincture preparation, its good effect in the treatment of experimental trichophytosis of guinea pigs, its low, acute and chronic toxicities and its lack of clinical side effects.View full abstractDownload PDF (898K) -
Kazuo MIURA, Takao UCHIYAMA, Koichiro HONDA1961 Volume 25 Issue 2 Pages 83-87
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSMany workers found that the poisoning animals with sodium fluoroacetate led quickly toa marked increase of citrate in the various tissues, but it failed to find such result in the liverin vivo as well as in vitro. In the liver of a frog, however, an accumulation of a large amountof citric acid was found 6 hours after the poison was orally administered to the animal. Todecide whether the accumulation of citric acid in the liver of a frog is due to the formation offluorocitrate in the liver itself or to the transport of fluorocitrate formed in other organs intothe liver, we carried out the experiments using the mitochondrial particles prepared from afrog liver. And it was found that the oxygen uptake of mitochondria was inhibited and thatthe citrate synthesis of the particle increased by the addition of fluoroacetate with pyruvateand malate as substrates.View full abstractDownload PDF (633K) -
Part I. Effects of Insulin Administration upon the Metabolism of Fluoroacetate in the Liver of a RatKazuo MIURA, Takao UCHIYAMA1961 Volume 25 Issue 2 Pages 87-93
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSIn a rat poisoned with sodium fluoroacetate no accumulation of citrate was found in theliver, while in that of a frog fluoroacetate administration was found to cause a markedincrease of citrate. In this paper, it was shown that even in a rat when it was treated withinsulin before fluoroacetate administration, citrate accumulation could be found in the liver. In the experiments in vitro using slices of a rat liver it was also shown that fluoroacetate couldcause an accumulation of citrate and inhibited the oxidation of malate in the slices of a normalas well as an insulinized liver.View full abstractDownload PDF (921K) -
Part II. Isolation and Identification of Monofluoroacetone BodiesKazuo MIURA, Takashi HORI1961 Volume 25 Issue 2 Pages 94-99
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSPrevious reports show that fluoroacetate could be activated even in a mammalian liver. To give a further evidence for the activation of fluoroacetate in the tissue, a rat liver isperfused with saline containing sodium fluoroacetate, and from the perfusion fluid monofluoroacetoneis separated as the 2, 4-dinitrophenylhydrazone, which is identified with the hydrazoneof synthetic monofluoroacetone on the phenoxyethanol-impregnated paper chromatogram.View full abstractDownload PDF (2896K) -
Part IV. Structure of Macrosporin (Group III)Rikisaku SUEMITSU, Minoru NAKAJIMA, Makoto HIURA1961 Volume 25 Issue 2 Pages 100-102
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSMacrosporin monomethylether was synthesized by means of condensation between α-resorcylic acid (I) and 3-methoxy-p-toluic acid (II) with the use of conc. sulfuric acid and boric anhydride.
From this result macrosporin C16H12O5, a metabolic product of Macrosporium porri Elliott, was established to be 3, 5-dihydroxy-7-methoxy-2-methyl anthraquinone (A).View full abstractDownload PDF (382K) -
Part VI. Further Studies on the Specificity of Crystalline Aspergitlus Saitoi ProteaseFumihiko YOSHIDA, Eiji ICHISHIMA1961 Volume 25 Issue 2 Pages 102-107
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSIn order to determine the specificity of Aspergillus Saitoi protease, the hydrolyzate of Bchainof insulin oxidized by this enzyme was investigated on paperchromatography accordingto the 2, 4-dinitrofluorobenzene technique. Specificity was compared with pepsin and other proteolytic enzymes.View full abstractDownload PDF (610K) -
Mitsuo NAMIKI, Yoshishige OKAZAWA, Akira MATSUYAMA1961 Volume 25 Issue 2 Pages 108-114
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSThe combined effects of inorganic reagents and radiation on the inactivation of E. coli in the resting state were studied. Among these reagents halides such as NaCl, KCl, KBr and KI were found to have a considerable synergistic action to radiation. Temperature effect on the halide action during irradiation was not observed, but removal of oxygen from halide solutions increased the radiosensitivity of cells. Combined effects of radiation and some other inorganic reagents were also investigated. Heavy metal salts and hydrogen peroxide were synergistic, nitrates and sulfates having no influence or a slightly protective action. Barium chloride and calcium chloride were protective in lower concentrations and synergistic in higher concentrations. These synergistic actions of inorganic reagents except ferric salts were observed during irradiation, but not after the irradiation.View full abstractDownload PDF (781K) -
Mitsuo NAMIKI, Yoshishige OKAZAWA, Akira MATSUYAMA1961 Volume 25 Issue 2 Pages 115-123
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSEffects of various chemical agents on the synergistic action of NaCl to the radiation inactivation of bacteria and yeast were studied. The remarkable modification of the radiation lethal effect by some reagents is considered to be a strong evidence for an indirect nature of NaCl synergistic action during irradiation. Most of these modification effects were restricted to the actions during irradiation, supporting the free radical hypothesis in which the short-life active species formed by radiation were considered to attack bacterial cells. Furthermore, preirradiation effects under various conditions suggest that the enhancement of radiation lethal effect by NaCl may involve the intracellular events.View full abstractDownload PDF (1150K) -
Part XI. Various Factors Affecting on Polyalcohol Production by Pichia misoHiroshi ONISHI, Narimasa SAITO, Ikunori KOSHIYAMA1961 Volume 25 Issue 2 Pages 124-130
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSVarious conditions were studied which gave influences on polyalcohol production byPichia miso.Pichia misogave an excellent yield of polyalcohol showing good growth in the vitamin-free medium. Unlike Zygosaccharomyceshigh concentrations of phosphate such as 2% as KH2PO4in the medium showed no detrimental effect on polyalcohol production. Remarkable reduction in polyalcohol yield was observed in the medium of high concentration of nitrogen sources, among which yeast extract showed the most striking effect. Polyalcohol fermentation was provoked in the medium of 0.1% yeast extract while the metabolic activity distinctly converted to ethanol fermentation when the organisms were incubated in the medium of 4.0% yeast extract. The fact that a large amount of ethanol more than 6% in the medium was produced aerobically by genusPichiaof oxidative dissimilation type seemed to be very interesting and noticeable. For the purpose of industrial production, it was shown that polyalcohol production in jar-fermenter scale was achieved with as good yield as that in shaking flask culture.View full abstractDownload PDF (1004K) -
Masahiro IWAIDA, Tomokichi TSUGO1961 Volume 25 Issue 2 Pages 130-136
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSSkim milk and Ca-caseinate solution were homogenized at various conditions by a Gaulintype homogenizer and the changes in nitrogen distribution were studied; from the results of which it was confirmed that a decrease in casein nitrogen and an increase in non-casein nitrogen occurred through homogenization, and that among the ingredients of non-casein nitrogen the increase of proteose-peptone nitrogen was comparatively large.
Casein particles are considered to include proteose-peptone as their components. When homogenized, a considerable amount of this proteose-peptone is set free through mechanical impact. This proteose-peptone is electrophoretically different from the proteose-peptone which originally exists in milk serum.View full abstractDownload PDF (1013K) -
Part XIII. Crystallization of Japanese-radish Peroxidase cYuhei MORITA, Katsuzo KAMEDA, Masayuki MIZUNO1961 Volume 25 Issue 2 Pages 136-140
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSPeroxidase c was isolated and purified from Japanese-radish roots by means of a chromatographic technique with carboxymethyl cellulose. Two or more components exhibiting the absorption spectrum of peroxidase c were separated chromatographically, and the most basic component was crystallized from ammonium sulphate solution. The Reinheit Zahl and the purpurogallin number of the crystalline preparation were found to be 3.55 and 1100 respectively. The absorption maxima were found at 420 and 540mμ for the oxidized form and at 425 and 560mμ for the reduced form. The crystalline preparation contained 1.57% protohematin as the prosthetic group, and then the minimum molecular weight of peroxidase c was found to be 41500.View full abstractDownload PDF (1856K) -
Part VI. Synergistic Activities of Phenolic LactonesKyôhei YAMASHITA, Masanao MATSUI1961 Volume 25 Issue 2 Pages 141-143
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSThe synergistic action of phenolic lactones on allethrin and pyrethrin were investigated from the mortality of synergized pyrethroids against rice-weevil by petri-dish method.
In the series of α-benzylidene-γ-butyrolactones, (±) hibalactone and α-piperonylidenebutyrolactone were appreciably synergistic on allethrin although less effective than piperonyl butoxide.(±) Hinokinin, 2-piperonylidene-3-piperonyl-1, 4-butanediol also showed week activation, but α-benzylidene-, α-anisylidene-, α-veratrylidene-butyrolactone, α-piperonylidene-α'-piperonyl-tetrahydrofuran, α-trimethoxybenzylidene-β-trimethoxybenzyl-butyrolactone were not synergistic on the insecticidal action.
In the series of synergized pyrethrin, (±) hibalactone and α-piperonylidene-butyrolactone showed week synergism but the other test compounds showed no appreciable synergism.View full abstractDownload PDF (344K) -
Hiroshi KUBO, Ichiro MUTA, Osami MATANO, Rokuro SATO1961 Volume 25 Issue 2 Pages 144-150
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSOur interest in the preparation of a new type of biologically active substances led us to investigate the reaction between organophosphates and organomercurials. O-Arylmercuric O, O-dialkyl thiophosphates (I) were synthesized by several methods from arylmercuric chloride and O, O-dialkyl thiophosphate in the presence of silver. Likewise several approaches to the synthesis of arylmercuric dialkyl thiophosphate (II) were made. Dialkyldithiophosphate and organomercurial reacted readily in several organic solvents to yield mercury bis (dialkyl dithiophosphate)(IV).View full abstractDownload PDF (621K) -
Part IV. Synthesis and Toxicity of Phosphorates and Phosphorothioates Containig 1-Phenyl-2-acyl-vinyl GroupYoshihiko NISHIZAWA1961 Volume 25 Issue 2 Pages 150-154
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSThe reaction of O, O-dialkyl phosphorochloridates or phosphorochloridothioates with sodium salts of benzoylacetone and the reaction of trialkyl phosphites with α-chloro-benzoylacetone or acetophenone were attempted with a view to prepare the low toxic phosphorus insecticides containing acylvinyl group. It was found that the products did not have a phosphonate form but a phosphorate-form and the phenyl-acylvinyl group had linkage with phosphorus atom by the enolform of next carbonyl group of the phenyl-group.View full abstractDownload PDF (630K) -
Part I. On Isolation of “Konjac” mannan-decomposing Enzyme, Mannase, from Human Gastrointestinal Bacteria.Satoshi INAMI1961 Volume 25 Issue 2 Pages 155-163
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSOn the gastrointestinal bacteria related to nutrition, there have been many importantpublications. Recently, the present author has attempted further researches on Aerobactermannanolyticus suzu-II, a strain in the type cultures collected by N. Inoue in this laboratoryfrom human gastrointestinal tract, and the enzyme specificity was studied. The isolation ofthe “Konjac” mannan decomposing enzyme preparation was completely successful, and effectsof carbon and nitrogen sources in Koser's citrate medium on the enzyme formation were also confirmed. In the present paper, the results are described.View full abstractDownload PDF (1029K) -
Part II. On Possible Metabolic Pathways in the Growth Response in Mannase Producing Bacterium, Aerobacter mannanolyticusSatoshi INAMI, Takao SUZUKI, Yoshikazu SAHASHI1961 Volume 25 Issue 2 Pages 164-169
Published: 1961
Released on J-STAGE: February 07, 2011
JOURNAL FREE ACCESSIn a previous paper, one of the authors (Innami) has reported the results of studies onthe isolation of mannase, “konjac” mannan-decomposing enzyme from the culture broth ofAerobacter mannanolyticus suzu-II, and if was confirmed that the enzyme preparation indicatedmarked activity for the decomposition of “konjac” mannan.
Afterwards, effects of various carbon sources on the growth response of the strain wasstudied, and it was found that the growth response of the bacteria may not be due to ordinaryTCA cycle members, whereas the strain did not respond to the media containing acetic acid, fumaric acid, succinic acid as a single carbon source. On the contrary, the bacteria increasedin the medium containing glyoxylate plus acetate as carbon sources. In conclusion, it wasclear that kornberg's glyoxylate-acetate cycle members formed by human digestive enzymesseem first to accelerate the growth response of the bacteria in the digestive tract, and successively, the mannase formed here to convert “konjac” mannan to D-glucose, D-mannose and others.View full abstractDownload PDF (728K)
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