Agricultural and Biological Chemistry
Online ISSN : 1881-1280
Print ISSN : 0002-1369
ISSN-L : 0002-1369
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Volume 26, Issue 4
Displaying 1-14 of 14 articles from this issue
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  • Biochemical Studies on Cycasin
    Part I. Purification and Properties of Cycad β-Glucosidase
    Akira KOBAYASHI
    1962 Volume 26 Issue 4 Pages 203-207
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.203
    JOURNAL FREE ACCESS
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    Purification of β-glucosidase from the seeds of Japanese cycad, and properties of the purified preparation are described. The enzyme activity was determined by colorimetry using ONPG as substrate. Crude preparation was obtained easily by adsorption on fibrous CMC pulp. It was further purified by chromatography on CMC powder, and a preparation which showed an activity of 135-folds of the original extract was obtained. Influences of pH, temperature, and substrate concentration upon the enzyme activity were examined. Michaelis constant of the enzyme for ONPG was 3.3×10-3M.
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  • Biochemical Studies on Cycasin
    Part II. Existence of Free Aglycon of Cycasin in Its Enzymatic Hydrolysis
    Akira KOBAYASHI
    1962 Volume 26 Issue 4 Pages 208-212
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.208
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    Some evidences indicating existence of free aglycon of cycasin are described. Cycasin was hydrolysed with purified cycad β-glucosidase, and the freed sugar component and aglycon were analyzed by manometry or quantitative paper chromatography and by spectrophotometry or polarography, respectively. When the hydrolysis was stopped by ice-cooling or byacidification, the reaction mixture showed the characteristic absorption spectrum and polarogram similar tothose of cycasin in spite of the liberation of glucose. However, if the reaction was stopped by heating, the spectrum and polarogram were not observed. This fact was considered to indicate that the free aglycon of cycasin formed in its enzymatic hydrolysis was decomposed by the heat treatment.
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  • Degradation of Nucleic Acids and Related Compounds by Microbial Enzymes
    Part X. 5'-Nucleotide Formation from Ribonucleic Acid by Bacterial Enzymes
    Atsushi KAKINUMA, Seizi IGARASI, Koichi OGATA
    1962 Volume 26 Issue 4 Pages 213-217
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.213
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    The culture filtrate of a strain of Bacillus subtilis decomposed ribonucleic acid into 5'-nucleotides and into other intermediates which released orthophosphate by an arsenateresistant phosphatase. Under the best conditions examined in these experiments, about 50 per cent of ribonucleic acid was converted into 5'-nucleotides.
    The culture filtrate of a strain of Bacillus brevis showed slight activities of ribonuclease and/or phosphodiesterase which produced 5'-nucleotides from ribonucleic acid, but showed predominant activity of 5'-adenylic acid degrading phosphatase.
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  • Degradation of Nucleic Acids and Related Compounds by Microbial Enzymes
    Part XI. Presence of 3'-Nucleotidase in the Culture Filtrate of Bacillus subtilis
    Seizi IGARASI, Atsushi KAKINUMA
    1962 Volume 26 Issue 4 Pages 218-220
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.218
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    The culture filtrate of Bacillus subtilis decomposed ribonucleic acid into adenosine and orthophosphate without passing through 5'-adenylic acid. It hydrolyzed neither 2'-nor 5'-nucleotides but only 3'-nucleotides into nucleoside and orthophosphate, while the cell suspension hydrolyzed 2'-, 3'-and 5'-nucleotides non-specifically. Thus 3'-nucleotidase was proved to exist in the culture filtrate of Bacillus subtilis.
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  • Degradation of Nucleic Acids and Related Compounds by Microbial Enzymes
    Part XII. 3'-Nucleotidase-forming Strains of Genus Bacillus
    Seizi IGARASI
    1962 Volume 26 Issue 4 Pages 221-223
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.221
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    All the tested strains of Bacillus subtilis produced 3'-nucleotidase in their culture filtrates, but other strains that belong to genus Bacillus produced very little amount of extracellular nucleotidase.
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  • Polarographic Studies on Fat-soluble Vitamins in Nonaqueous Media
    Part I. Polarography of Vitamin K3 in Acetonitrile as Solvent
    Tomotoshi OKAICHI, Yoshiro HASHIMOTO
    1962 Volume 26 Issue 4 Pages 224-227
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.224
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    When treated with Raney nickel, S-bisbenzoyl derivative of dihydronereistoxin gave N, N-dimethylisopropylamine together with dimethylamine. Supported by other evidences, especially on the presence of 1, 2-dithiolane ring, this led us to postulate that nereistoxin is 4-N, N-dimethylamino-1, 2-dithiolane. The structure was also unequivocally supported by the NMR spectrum. Some additional data on chemical property of nereistoxin and its derivatives were described in succession to the previous paper.
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  • Studies on Streptomycetes
    Part I. Streptumyces atratus nov. sp., Producing New Antituberculous Antibiotics Rufomycin A and B
    Motoo SHIBATA, Eiji HIGASHIDE, Hiroichi YAMAMOTO, Koiti NAKAZAWA
    1962 Volume 26 Issue 4 Pages 228-233
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.228
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    Investigation was made on the mycological and antibacterial properties of a strain No.46408 isolated from a soil sample collected in Shimoueda, Wakayama Prefecture. The strain No.46408 was compared with similar strains, St. hygroscopicus and St. halstedii, and it was judged to belong to a new species and therefore named Streptomyces atratus nov. sp. Also a strain A-165-Z1, which produces ilamycin, was referred to on its mycological properties and assumed to resemble St. atratus. St. atratus was found to produce new antibiotics, rufomycin A and B, specially active against acid-fast bacteria.
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  • Studies on Streptomycetes
    Part II. Rufomycin A and B, New Antituberculous Antibiotics
    Eiji HIGASHIDE, Motoo SHIBATA, Hiroichi YAMAMOTO, Koiti NAKAZAWA, Hide ...
    1962 Volume 26 Issue 4 Pages 234-237
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.234
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    Investigation was made on the isolation procedure, physico-chemical properties and biological activities of antibiotic 46408 produced by Streptomyces atratus nov. sp. The solvent extract from the whole culture of this strain gave two antibiotic substances. Both are yellow, neutral and sparingly soluble in water and active against acid-fast bacteria, especially Mycobacterium tuberculosis and Mycobacterium smegmatis but inactive against most other bacteria, fungi and yeast examined.
    From the physico-chemical and microbiological properties they were found to be new substances and named rufomycin A and B, respectively.
    Recently Umezawa et al. reported on new antibiotics, ilamycins, active against acidfast bacteria. The properties of rufomycins and ilamycins suggest that they really are very much like. Rufomycin A was nearly as effective as streptomycin in experimental tuberculosis in mice.
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  • Polarographic Studies on Fat-soluble Vitamins in Nonaqueous Media
    Part I. Polarography of Vitamin K3 in Acetonitrile as Solvent
    Isamu TACHI, Reiji TAKAHASHI
    1962 Volume 26 Issue 4 Pages 238-244
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.238
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    Polarographic reduction-wave of vitamin K3 showed two inflexions in acetonitrile as solvent. The first step inflexion is caused by reduction of quinone to semiquinone stabilized anion free-radical in acetonitrile, and the second by reduction of semiquinone to hydroquinone dianion. With small addition of water, the dismutation of semiquinone is accelerated and the reduction-wave becomes only one step inflexion. The reduction-wave in acetonitrile is controlled by liffusion. The total wave-height is strictly proportional to concentration of vitamin K3. The measurement is not disturbed by coexistent fatty substances.
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  • Studies on Osmophilic Yeasts
    Part XIV. Partial Purification of Polyol Dehydrogeuase from Pichia miso and the Properties of this Enzymes
    Hiroshi ONISHI, Narimasa SAITO
    1962 Volume 26 Issue 4 Pages 245-251
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.245
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    In order to ascertain the existence of polyol dehydrogenase which seemed to take a part in polyalcohol production by Pichia miso, the preparation and the partial purification of polyol dehydrogenase from thecells of Pichia miso were carried out. Some properties of this enzyme preparation and the identification of the products formed by this enzyme action were also described. This enzyme preparation was found to catalyze the following reactions:
    D-arabitol+DPN+⇔D-Xylulose+DPNH+H+, Polyalcohol+DPN+⇔Ketose+DPNH+H+.
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  • Studies on Organophosphorus Insecticides
    Part IX. Preparation and Biological Properties of Phenylphosphonothioates
    Hideo SAKAMOTO, Yoshihiko NISHIZAWA
    1962 Volume 26 Issue 4 Pages 252-256
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.252
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    Various insecticides belonging to the O-alkyl-O-(subst. phenyl) phenylphosphonothioate family were prepared and their biological activities studied. Besides O-ethyl-O-(4-nitrophenyl) phenylphosphonothioate, which is utilized in these days, another compound, O-ethyl-O-(4-cyanophenyl) phenylphosphonothioate, proved to have excellent properties as an insecticide.
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  • Studies on Organophosphorus Insecticides
    Part X. Chemical and Biological Properties of O, O-Dimethyl-O-(4-cyanophenyl) phosphorothioate and O-Ethyl-O-(4-cyanophenyl) phenylphosphonothioate
    Yoshihiko NISHIZAWA, Saichiro KURAMOTO, Tadaomi KADOTA, Junshi MIYAMOT ...
    1962 Volume 26 Issue 4 Pages 257-264
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.257
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    In our previous paper, it was reported that O, O-dimethyl-O-(4-cyanophenyl) phosphorothioate (S-4084) has only a low toxicity towards mammals though possessing a high activity towards rice stem borers. In this paper, the chemical and biological properties of S-4084 and its phenylphosphonothioate derivative (S-4087) will be reported in detail. It should especially be noted that S-4084 had a higher activity towards rice stem borers than S-4087 by topical or pot test in a room or in a green-house, but by field test S-4087 was stronger than S-4084.
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  • Studies on the Biosynthesis of Theanine in Tea Seedlings Incorporation of Glutamic Acid-l-14C into Theanine
    Kei SASAOKA, Makoto KITO, Shigeki KONISHI, Hiroko INAGAKI
    1962 Volume 26 Issue 4 Pages 265-266
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.265
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  • Studies on Conjugated Lipids
    Part XVI. Configuration of Biosynthesized Sphingosine
    Yasuhiko FUJINO, Irving ZABIN
    1962 Volume 26 Issue 4 Pages 267-268
    Published: 1962
    Released on J-STAGE: February 07, 2011
    DOIhttps://doi.org/10.1271/bbb1961.26.267
    JOURNAL FREE ACCESS
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